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Article

Circadian clock gene Per2 plays an important role in cell proliferation, apoptosis and cell cycle progression in human oral squamous cell carcinoma

  • Authors:
    • Qingqing Wang
    • Yiran Ao
    • Kai Yang
    • Hong Tang
    • Dan Chen
  • View Affiliations / Copyright

    Affiliations: Stomatological Hospital of Chongqing Medical University, Chongqing 400017, P.R. China, Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 3387-3394
    |
    Published online on: April 1, 2016
       https://doi.org/10.3892/or.2016.4724
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Abstract

Previous studies have shown that the aberrant expression of period circadian clock 2 (Per2) is closely related to the occurrence and development of cancers, but the specific mechanism remains unclear. In the present study, we used shRNA to downregulate Per2 in oral squamous cell carcinoma (OSCC) Tca8113 cells, and then detected the alterations in cell cycle, cell proliferation and apoptosis by flow cytometric analysis and mRNA expression alterations in all the important genes in the cyclin/cyclin-dependent protein kinase (CDK)/cyclin-dependent kinase inhibitor (CKI) cell cycle network by RT-qPCR. We found that in the Tca8113 cells, after Per2 downregulation, the mRNA expression levels of cyclin A2, B1 and D1, CDK4, CDK6 and E2F1 were significantly increased (P<0.05), the mRNA expression levels of p53, p16 and p21 were significantly decreased (P<0.05), cell proliferation was significantly higher (P<0.05), apoptosis was significantly lower (P<0.05) and the number of cells in the G1/G0 phase was significantly decreased (P<0.05). The present study proves that in OSCC, clock gene Per2 plays an important role in cell cycle progression and the balance of cell proliferation and apoptosis by regulation of the cyclin/CDK/CKI cell cycle network. Further research on Per2 may provide a new effective molecular target for cancer treatments.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Q, Ao Y, Yang K, Tang H and Chen D: Circadian clock gene Per2 plays an important role in cell proliferation, apoptosis and cell cycle progression in human oral squamous cell carcinoma. Oncol Rep 35: 3387-3394, 2016.
APA
Wang, Q., Ao, Y., Yang, K., Tang, H., & Chen, D. (2016). Circadian clock gene Per2 plays an important role in cell proliferation, apoptosis and cell cycle progression in human oral squamous cell carcinoma. Oncology Reports, 35, 3387-3394. https://doi.org/10.3892/or.2016.4724
MLA
Wang, Q., Ao, Y., Yang, K., Tang, H., Chen, D."Circadian clock gene Per2 plays an important role in cell proliferation, apoptosis and cell cycle progression in human oral squamous cell carcinoma". Oncology Reports 35.6 (2016): 3387-3394.
Chicago
Wang, Q., Ao, Y., Yang, K., Tang, H., Chen, D."Circadian clock gene Per2 plays an important role in cell proliferation, apoptosis and cell cycle progression in human oral squamous cell carcinoma". Oncology Reports 35, no. 6 (2016): 3387-3394. https://doi.org/10.3892/or.2016.4724
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Q, Ao Y, Yang K, Tang H and Chen D: Circadian clock gene Per2 plays an important role in cell proliferation, apoptosis and cell cycle progression in human oral squamous cell carcinoma. Oncol Rep 35: 3387-3394, 2016.
APA
Wang, Q., Ao, Y., Yang, K., Tang, H., & Chen, D. (2016). Circadian clock gene Per2 plays an important role in cell proliferation, apoptosis and cell cycle progression in human oral squamous cell carcinoma. Oncology Reports, 35, 3387-3394. https://doi.org/10.3892/or.2016.4724
MLA
Wang, Q., Ao, Y., Yang, K., Tang, H., Chen, D."Circadian clock gene Per2 plays an important role in cell proliferation, apoptosis and cell cycle progression in human oral squamous cell carcinoma". Oncology Reports 35.6 (2016): 3387-3394.
Chicago
Wang, Q., Ao, Y., Yang, K., Tang, H., Chen, D."Circadian clock gene Per2 plays an important role in cell proliferation, apoptosis and cell cycle progression in human oral squamous cell carcinoma". Oncology Reports 35, no. 6 (2016): 3387-3394. https://doi.org/10.3892/or.2016.4724
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