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Article

Genome-wide profiling of long non-coding RNA expression patterns in the EGFR-TKI resistance of lung adenocarcinoma by microarray

  • Authors:
    • Ying Wu
    • Dan-Dan Yu
    • Yong Hu
    • Dali Yan
    • Xiu Chen
    • Hai‑Xia Cao
    • Shao-Rong Yu
    • Zhuo Wang
    • Ji-Feng Feng
  • View Affiliations / Copyright

    Affiliations: The First Clinical School of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China, Department of Chemotherapy, Nanjing Medical University Affiliated Cancer Hospital Cancer Institute of Jiangsu Province, Nanjing, Jiangsu 210009, P.R. China, The Fourth Clinical School of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 3371-3386
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    Published online on: April 20, 2016
       https://doi.org/10.3892/or.2016.4758
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Abstract

Mutations in the epidermal growth factor receptor (EGFR) make lung adenocarcinoma cells sensitive to EGFR tyrosine kinase inhibitors (TKIs). Long-term cancer therapy may cause the occurrence of acquired resistance to EGFR TKIs. Long non-coding RNAs (lncRNAs) play important roles in tumor formation, tumor metastasis and the development of EGFR-TKI resistance in lung cancer. To gain insight into the molecular mechanisms of EGFR-TKI resistance, we generated an EGFR-TKI-resistant HCC827-8-1 cell line and analyzed expression patterns by lncRNA microarray and compared it with its parental HCC827 cell line. A total of 1,476 lncRNA transcripts and 1,026 mRNA transcripts were dysregulated in the HCC827‑8-1 cells. The expression levels of 7 chosen lncRNAs were validated by real-time quantitative PCR. As indicated by functional analysis, several groups of lncRNAs may be involved in the bio-pathways associated with EGFR-TKI resistance through their cis- and/or trans‑regulation of protein-coding genes. Thus, lncRNAs may be used as novel candidate biomarkers and potential targets in EGFR-TKI therapy in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Wu Y, Yu D, Hu Y, Yan D, Chen X, Cao HX, Yu S, Wang Z and Feng J: Genome-wide profiling of long non-coding RNA expression patterns in the EGFR-TKI resistance of lung adenocarcinoma by microarray. Oncol Rep 35: 3371-3386, 2016.
APA
Wu, Y., Yu, D., Hu, Y., Yan, D., Chen, X., Cao, H. ... Feng, J. (2016). Genome-wide profiling of long non-coding RNA expression patterns in the EGFR-TKI resistance of lung adenocarcinoma by microarray. Oncology Reports, 35, 3371-3386. https://doi.org/10.3892/or.2016.4758
MLA
Wu, Y., Yu, D., Hu, Y., Yan, D., Chen, X., Cao, H., Yu, S., Wang, Z., Feng, J."Genome-wide profiling of long non-coding RNA expression patterns in the EGFR-TKI resistance of lung adenocarcinoma by microarray". Oncology Reports 35.6 (2016): 3371-3386.
Chicago
Wu, Y., Yu, D., Hu, Y., Yan, D., Chen, X., Cao, H., Yu, S., Wang, Z., Feng, J."Genome-wide profiling of long non-coding RNA expression patterns in the EGFR-TKI resistance of lung adenocarcinoma by microarray". Oncology Reports 35, no. 6 (2016): 3371-3386. https://doi.org/10.3892/or.2016.4758
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Spandidos Publications style
Wu Y, Yu D, Hu Y, Yan D, Chen X, Cao HX, Yu S, Wang Z and Feng J: Genome-wide profiling of long non-coding RNA expression patterns in the EGFR-TKI resistance of lung adenocarcinoma by microarray. Oncol Rep 35: 3371-3386, 2016.
APA
Wu, Y., Yu, D., Hu, Y., Yan, D., Chen, X., Cao, H. ... Feng, J. (2016). Genome-wide profiling of long non-coding RNA expression patterns in the EGFR-TKI resistance of lung adenocarcinoma by microarray. Oncology Reports, 35, 3371-3386. https://doi.org/10.3892/or.2016.4758
MLA
Wu, Y., Yu, D., Hu, Y., Yan, D., Chen, X., Cao, H., Yu, S., Wang, Z., Feng, J."Genome-wide profiling of long non-coding RNA expression patterns in the EGFR-TKI resistance of lung adenocarcinoma by microarray". Oncology Reports 35.6 (2016): 3371-3386.
Chicago
Wu, Y., Yu, D., Hu, Y., Yan, D., Chen, X., Cao, H., Yu, S., Wang, Z., Feng, J."Genome-wide profiling of long non-coding RNA expression patterns in the EGFR-TKI resistance of lung adenocarcinoma by microarray". Oncology Reports 35, no. 6 (2016): 3371-3386. https://doi.org/10.3892/or.2016.4758
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