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Article

Myricetin suppresses p21-activated kinase 1 in human breast cancer MCF-7 cells through downstream signaling of the β-catenin pathway

  • Authors:
    • De Jiao
    • Xue Dong Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Breast and Thyroid Hernia Surgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China, Department of Pathology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China
  • Pages: 342-348
    |
    Published online on: April 28, 2016
       https://doi.org/10.3892/or.2016.4777
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Abstract

As a main active compound in the bark of waxberry (Myrica rubra), myricetin is a macrocyclic diarylheptanoid, and can trigger the apoptosis of HeLa and PC3 cells. The aim of the present study was to elucidate the anticancer effect of myricetin on human breast cancer MCF-7 cells and to explore the possible mechanisms of action. MCF-7 cells were treated with different concentrations of myricetin (0-80 µM) for 12, 24 and 48 h. In the present study, we found that myricetin suppressed the cell viability of the MCF-7 cells at least partly through the induction of apoptosis as determined by MTT assay and flow cytometry. Western blot analysis revealed that myricetin effectively suppressed the protein expression of p21-activated kinase 1 (PAK1), MEK and phosphorylated extracellular mitogen-activated protein kinase (ERK1/2). In addition, treatment of myricetin activated glycogen synthase kinase-3β (GSK3β) and Bax protein expression, and inhibited β-catenin/cyclin D1/proliferating cell nuclear antigen (PCNA)/survivin and promoted caspase-3 activity in the MCF-7 cells. These results demonstrated that myricetin suppressed the cell viability of human breast cancer MCF-7 cells through PAK1/MEK/ERK/GSK3β/β-catenin/cyclin D1/PCNA/survivin/Bax-caspase-3 signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Jiao D and Zhang XD: Myricetin suppresses p21-activated kinase 1 in human breast cancer MCF-7 cells through downstream signaling of the β-catenin pathway. Oncol Rep 36: 342-348, 2016.
APA
Jiao, D., & Zhang, X.D. (2016). Myricetin suppresses p21-activated kinase 1 in human breast cancer MCF-7 cells through downstream signaling of the β-catenin pathway. Oncology Reports, 36, 342-348. https://doi.org/10.3892/or.2016.4777
MLA
Jiao, D., Zhang, X. D."Myricetin suppresses p21-activated kinase 1 in human breast cancer MCF-7 cells through downstream signaling of the β-catenin pathway". Oncology Reports 36.1 (2016): 342-348.
Chicago
Jiao, D., Zhang, X. D."Myricetin suppresses p21-activated kinase 1 in human breast cancer MCF-7 cells through downstream signaling of the β-catenin pathway". Oncology Reports 36, no. 1 (2016): 342-348. https://doi.org/10.3892/or.2016.4777
Copy and paste a formatted citation
x
Spandidos Publications style
Jiao D and Zhang XD: Myricetin suppresses p21-activated kinase 1 in human breast cancer MCF-7 cells through downstream signaling of the β-catenin pathway. Oncol Rep 36: 342-348, 2016.
APA
Jiao, D., & Zhang, X.D. (2016). Myricetin suppresses p21-activated kinase 1 in human breast cancer MCF-7 cells through downstream signaling of the β-catenin pathway. Oncology Reports, 36, 342-348. https://doi.org/10.3892/or.2016.4777
MLA
Jiao, D., Zhang, X. D."Myricetin suppresses p21-activated kinase 1 in human breast cancer MCF-7 cells through downstream signaling of the β-catenin pathway". Oncology Reports 36.1 (2016): 342-348.
Chicago
Jiao, D., Zhang, X. D."Myricetin suppresses p21-activated kinase 1 in human breast cancer MCF-7 cells through downstream signaling of the β-catenin pathway". Oncology Reports 36, no. 1 (2016): 342-348. https://doi.org/10.3892/or.2016.4777
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