Skp2 is associated with paclitaxel resistance in prostate cancer cells

  • Authors:
    • Yeguo Yang
    • Yi Lu
    • Lihui Wang
    • Atsushi Mizokami
    • Evan T. Keller
    • Jian Zhang
    • Jiejun Fu
  • View Affiliations

  • Published online on: May 13, 2016     https://doi.org/10.3892/or.2016.4809
  • Pages: 559-566
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Abstract

Prostate cancer is the most commonly diagnosed tumor in men in the United States. Patients with hormone-refractory prostate cancer are often treated with paclitaxel, but most of them eventually develop drug resistance. S-phase kinase associated protein 2 (Skp2) is a component of the SCF (Skp1-Cullin1-F-box) type of E3 ubiquitin ligase complexes. In the present study, we investigated the role of Skp2 in paclitaxel-resistant DU145-TxR or PC-3-TxR cells by Skp2 silencing or using Skp2 inhibitors. We first confirmed that Skp2 expression is up­regulated in DU145-TxR or PC-3-TxR cells compared with their parental cells DU145 or PC-3, respectively. Knockdown of Skp2 or Skp2 inhibitor treatment in DU145-TxR or PC-3-TxR cells restored paclitaxel sensitivity. E-cadherin was decreased while Vimentin was increased in PC-3-TxR or DU145-TxR cells. In addition, p27 expression was inversely correlated with Skp2 expression in DU145-TxR or PC-3-TxR cells. Moreover, p27 was found to increase in both Skp2 silencing PC-3-TxR and DU145-TxR cells. These results suggest that Skp2 is associated with prostate cancer cell resistance to paclitaxel. Skp2 may be a potential therapeutic target for drug-resistant prostate cancer.
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July-2016
Volume 36 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yang Y, Lu Y, Wang L, Mizokami A, Keller ET, Zhang J and Fu J: Skp2 is associated with paclitaxel resistance in prostate cancer cells. Oncol Rep 36: 559-566, 2016
APA
Yang, Y., Lu, Y., Wang, L., Mizokami, A., Keller, E.T., Zhang, J., & Fu, J. (2016). Skp2 is associated with paclitaxel resistance in prostate cancer cells. Oncology Reports, 36, 559-566. https://doi.org/10.3892/or.2016.4809
MLA
Yang, Y., Lu, Y., Wang, L., Mizokami, A., Keller, E. T., Zhang, J., Fu, J."Skp2 is associated with paclitaxel resistance in prostate cancer cells". Oncology Reports 36.1 (2016): 559-566.
Chicago
Yang, Y., Lu, Y., Wang, L., Mizokami, A., Keller, E. T., Zhang, J., Fu, J."Skp2 is associated with paclitaxel resistance in prostate cancer cells". Oncology Reports 36, no. 1 (2016): 559-566. https://doi.org/10.3892/or.2016.4809