Zidovudine, abacavir and lamivudine increase the radiosensitivity of human esophageal squamous cancer cell lines

Chen,Xuan; Wang,Cong; Guan,Shanghui; Liu,Yuan; Han,Lihui; Cheng,Yufeng

doi:10.3892/or.2016.4819

Zidovudine, abacavir and lamivudine increase the radiosensitivity of human esophageal squamous cancer cell lines

Authors:
- Xuan Chen
- Cong Wang
- Shanghui Guan
- Yuan Liu
- Lihui Han
- Yufeng Cheng
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Affiliations: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
Published online on: May 18, 2016 https://doi.org/10.3892/or.2016.4819
Pages: 239-246

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Abstract

Telomerase is a type of reverse transcriptase that is overexpressed in almost all human tumor cells, but not in normal tissues, which provides an opportunity for radiosensitization targeting telomerase. Zidovudine, abacavir and lamivudine are reverse transcriptase inhibitors that have been applied in clinical practice for several years. We sought to explore the radiosensitization effect of these three drugs on human esophageal cancer cell lines. Eca109 and Eca9706 cells were treated with zidovudine, abacavir and lamivudine for 48 h before irradiation was administered. Samples were collected 1 h after irradiation. Clonal efficiency assay was used to evaluate the effect of the combination of these drugs with radiation doses of 2, 4, 6 and 8 Gy. DNA damage was measured by comet assay. Telomerase activity (TA) and relative telomere length (TL) were detected and evaluated by real-time PCR. Apoptosis rates were assessed by flow cytometric analysis. The results showed that all the drugs tested sensitized the esophageal squamous cell carcinoma (ESCC) cell lines to radiation through an increase in radiation-induced DNA damage and cell apoptosis, deregulation of TA and decreasing the shortened TL caused by radiation. Each of the drugs investigated (zidovudine, abacavir and lamivudine) could be used for sensitizing human esophageal cancer cell lines to radiation. Consequently, the present study supports the potential of these three drugs as therapeutic agents for the radiosensitization of esophageal squamous cell cancer.

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