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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Article

Effects of TMEM9 gene on cell progression in hepatocellular carcinoma by RNA interference

  • Authors:
    • Yi Zhang
    • Yan Ran
    • Yan Xiong
    • Zi-Biao Zhong
    • Zheng‑Hua Wang
    • Xiao-Li Fan
    • Qi-Fa Ye
  • View Affiliations / Copyright

    Affiliations: Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan University, Wuchang, Hubei 430071, P.R. China, Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Department of Anesthesiology, Wuhan University, Wuchang, Hubei 430071, P.R. China
  • Pages: 299-305
    |
    Published online on: May 19, 2016
       https://doi.org/10.3892/or.2016.4821
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Abstract

Hepatocellular carcinoma (HCC) is a malignant tumor that has become a global health issue. The aim of the present study was to examine the role of transmembrane protein 9 (TMEM9) in cell progression, such as cell growth, cell cycle, cell metastasis of hepatoma cells, and to discuss the TMEM9 gene‑encoding protein as a potential therapy target of hepatoma. RT-qPCR was performed to examine TMEM9 expression in tumor tissues and adjacent tissues of patients with liver cancer. siRNAs were used to interfere TMEM9 in HepG2 and 7721 cells. A CCK-8 assay was performed to evaluate cell growth at 24, 48 and 72 h. Cell cycle and apoptosis were analyzed using flow cytometry. Transwell assays were used to determine cell invasion, migration and adhesion. The results showed that TMEM9 was expressed abnormally in liver cancers. TMEM9 expression increased significantly in the 34 examined patients. TMEM9 knockdown inhibited proliferation in the HepG2 and 7721 cells. The flow cytometric analysis revealed that TMEM9 knockdown by RNA interference resulted in G1 arrest and induced apoptosis. Cell invasion, migration and adhesion ability were also decreased. Western blotting indicated that expression of the cell cycle‑related proteins CDK1, EIF3H, RPL10L, S100A10, CCNB1 and CCNB2 was significantly decreased. In conclusion, TMEM9 plays an important role in the cell growth of hepatoma cells.
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View References

1 

Mathers C, Fat DM and Boerma JT: The Global Burden of Disease: 2004 Update. World Health Organization; 2008

2 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Bosch FX, Ribes J, Díaz M and Cléries R: Primary liver cancer: Worldwide incidence and trends. Gastroenterology. 127(Suppl 1): S5–S16. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Villanueva A, Newell P, Chiang DY, Friedman SL and Llovet JM: Genomics and signaling pathways in hepatocellular carcinoma. Semin Liver Dis. 27:55–76. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Yuen MF, Tanaka Y, Fong DY-T, Fung J, Wong DK, Yuen JC, But DY, Chan AO, Wong BC, Mizokami M, et al: Independent risk factors and predictive score for the development of hepatocellular carcinoma in chronic hepatitis B. J Hepatol. 50:80–88. 2009. View Article : Google Scholar

6 

Yang HI, Sherman M, Su J, Chen PJ, Liaw YF, Iloeje UH and Chen CJ: Nomograms for risk of hepatocellular carcinoma in patients with chronic hepatitis B virus infection. J Clin Oncol. 28:2437–2444. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Lok AS, Seeff LB, Morgan TR, di Bisceglie AM, Sterling RK, Curto TM, Everson GT, Lindsay KL, Lee WM, Bonkovsky HL, et al HALT-C Trial Group: Incidence of hepatocellular carcinoma and associated risk factors in hepatitis C-related advanced liver disease. Gastroenterology. 136:138–148. 2009. View Article : Google Scholar

8 

Yeoman AD, Al-Chalabi T, Karani JB, Quaglia A, Devlin J, Mieli-Vergani G, Bomford A, O'Grady JG, Harrison PM and Heneghan MA: Evaluation of risk factors in the development of hepatocellular carcinoma in autoimmune hepatitis: Implications for follow-up and screening. Hepatology. 48:863–870. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Wang X, Wang N, Cheung F, Lao L, Li C and Feng Y: Chinese medicines for prevention and treatment of human hepatocellular carcinoma: Current progress on pharmacological actions and mechanisms. J Integr Med. 13:142–164. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Lopez PM, Villanueva A and Llovet JM: Systematic review: Evidence-based management of hepatocellular carcinoma - an updated analysis of randomized controlled trials. Aliment Pharmacol Ther. 23:1535–1547. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Villanueva A, Toffanin S and Llovet JM: Linking molecular classification of hepatocellular carcinoma and personalized medicine: Preliminary steps. Curr Opin Oncol. 20:444–453. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Ke AW, Shi GM, Zhou J, Huang XY, Shi YH, Ding ZB, Wang XY, Devbhandari RP and Fan J: CD151 amplifies signaling by integrin α6β1 to PI3K and induces the epithelial-mesenchymal transition in HCC Cells. Gastroenterology. 140:1629–1641.e15. 2011. View Article : Google Scholar

13 

Kveine M, Tenstad E, Døsen G, Funderud S and Rian E: Characterization of the novel human transmembrane protein 9 (TMEM9) that localizes to lysosomes and late endosomes. Biochem Biophys Res Commun. 297:912–917. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar

15 

Fang F, Yang L, Tao Y and Qin W: FBI-1 promotes cell proliferation and enhances resistance to chemotherapy of hepatocellular carcinoma in vitro and in vivo. Cancer. 118:134–146. 2012. View Article : Google Scholar

16 

Zhai XF, Chen Z, Li B, Shen F, Fan J, Zhou WP, Yang YK, Xu J, Qin X, Li LQ and Ling CQ: Traditional herbal medicine in preventing recurrence after resection of small hepatocellular carcinoma: a multicenter randomized controlled trial. J Integr Med. 11:90–100. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Morgan DO: The Cell Cycle: Principles of Control. New Science Press. 2007.

18 

Spilka R, Ernst C, Mehta AK and Haybaeck J: Eukaryotic translation initiation factors in cancer development and progression. Cancer Lett. 340:9–21. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Okamoto H, Yasui K, Zhao C, Arii S and Inazawa J: PTK2 and EIF3S3 genes may be amplification targets at 8q23–q24 and are associated with large hepatocellular carcinomas. Hepatology. 38:1242–1249. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Cappuzzo F, Varella-Garcia M, Rossi E, Gajapathy S, Valente M, Drabkin H and Gemmill R: MYC and EIF3H coamplification significantly improve response and survival of non-small cell lung cancer patients (NSCLC) treated with gefitinib. J Thorac Oncol. 4:472–478. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Jackman M, Firth M and Pines J: Human cyclins B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus. EMBO J. 14:1646–1654. 1995.PubMed/NCBI

22 

Porter LA, Singh G and Lee JM: Abundance of cyclin B1 regulates γ-radiation-induced apoptosis. Blood. 95:2645–2650. 2000.

23 

Rescher U and Gerke V: S100A10/p11: Family, friends and functions. Pflugers Arch. 455:575–582. 2008. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Ran Y, Xiong Y, Zhong Z, Wang ZH, Fan X and Ye Q: Effects of TMEM9 gene on cell progression in hepatocellular carcinoma by RNA interference. Oncol Rep 36: 299-305, 2016.
APA
Zhang, Y., Ran, Y., Xiong, Y., Zhong, Z., Wang, Z., Fan, X., & Ye, Q. (2016). Effects of TMEM9 gene on cell progression in hepatocellular carcinoma by RNA interference. Oncology Reports, 36, 299-305. https://doi.org/10.3892/or.2016.4821
MLA
Zhang, Y., Ran, Y., Xiong, Y., Zhong, Z., Wang, Z., Fan, X., Ye, Q."Effects of TMEM9 gene on cell progression in hepatocellular carcinoma by RNA interference". Oncology Reports 36.1 (2016): 299-305.
Chicago
Zhang, Y., Ran, Y., Xiong, Y., Zhong, Z., Wang, Z., Fan, X., Ye, Q."Effects of TMEM9 gene on cell progression in hepatocellular carcinoma by RNA interference". Oncology Reports 36, no. 1 (2016): 299-305. https://doi.org/10.3892/or.2016.4821
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Ran Y, Xiong Y, Zhong Z, Wang ZH, Fan X and Ye Q: Effects of TMEM9 gene on cell progression in hepatocellular carcinoma by RNA interference. Oncol Rep 36: 299-305, 2016.
APA
Zhang, Y., Ran, Y., Xiong, Y., Zhong, Z., Wang, Z., Fan, X., & Ye, Q. (2016). Effects of TMEM9 gene on cell progression in hepatocellular carcinoma by RNA interference. Oncology Reports, 36, 299-305. https://doi.org/10.3892/or.2016.4821
MLA
Zhang, Y., Ran, Y., Xiong, Y., Zhong, Z., Wang, Z., Fan, X., Ye, Q."Effects of TMEM9 gene on cell progression in hepatocellular carcinoma by RNA interference". Oncology Reports 36.1 (2016): 299-305.
Chicago
Zhang, Y., Ran, Y., Xiong, Y., Zhong, Z., Wang, Z., Fan, X., Ye, Q."Effects of TMEM9 gene on cell progression in hepatocellular carcinoma by RNA interference". Oncology Reports 36, no. 1 (2016): 299-305. https://doi.org/10.3892/or.2016.4821
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