miR-410-3p suppresses breast cancer progression by targeting Snail

Zhang,Ya-Feng; Yu,Yue; Song,Wang-Zhao; Zhang,Rui-Ming; Jin,Shan; Bai,Jun-Wen; Kang,Hong-Bin; Wang,Xin; Cao,Xu-Chen

doi:10.3892/or.2016.4828

miR-410-3p suppresses breast cancer progression by targeting Snail

Authors:
- Ya-Feng Zhang
- Yue Yu
- Wang-Zhao Song
- Rui-Ming Zhang
- Shan Jin
- Jun-Wen Bai
- Hong-Bin Kang
- Xin Wang
- Xu-Chen Cao
View Affiliations

Affiliations: The First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, P.R. China, Department of Surgery, Affiliated Hospital, Inner Mongolia Medical University, Inner Mongolia 010050, P.R. China
Published online on: May 23, 2016 https://doi.org/10.3892/or.2016.4828
Pages: 480-486

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

miR-410-3p acts as an oncogene or tumor-suppressor gene in various types of cancer. However, its role in breast cancer remains unknown. In the present study, expression of miR-410-3p in 30 breast cancer and paired adjacent normal tissues was detected by RT-qPCR. The expression of miR-410-3p was downregulated in 76.7% of the breast cancer samples. To further validate the expression of miR-410-3p in breast cancer, we analyzed miR-410-3p expression profiling data set from The Cancer Genome Atlas (TCGA) including 683 breast cancer and 87 normal breast tissues. We observed that the expression of miR-410-3p was downregulated in breast cancer tissues. Next, we investigated the influence of miR-410-3p on cell proliferation by transiently transfecting the miR-410-3p mimic or inhibitor, as well as their corresponding controls in the MDA-MB-231 and MCF7 cell lines. miR-410-3p overexpression reduced cell growth, colony formation and the number of EdU-positive cells in the MDA-MB-231 cells. In contrast, inhibition of miR-410-3p in the MCF7 cells resulted in a higher proliferation rate as assessed by MTT assay, plate colony formation and EdU assays. Furthermore, miR-410-3p inhibited epithelial-mesenchymal transition. In addition, Snail was found to be a direct target of miR-410-3p based on a luciferase assay. Overexpression of Snail was able to rescue the effect of miR-410-3p in breast cancer cells. Moreover, miR‑410-3p was inversely expressed with Snail in breast cancer samples. Our data provide new knowledge regarding the role of miR-410-3p in breast cancer progression.

View Figures

View References

1	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI
2	Cortés-Sempere M and Ibáñez de Cáceres I: microRNAs as novel epigenetic biomarkers for human cancer. Clin Transl Oncol. 13:357–362. 2011. View Article : Google Scholar : PubMed/NCBI
3	Sreekumar R, Sayan BS, Mirnezami AH and Sayan AE: MicroRNA control of invasion and metastasis pathways. Front Genet. 2:582011. View Article : Google Scholar
4	Shi KQ, Lin Z, Chen XJ, Song M, Wang YQ, Cai YJ, Yang NB, Zheng MH, Dong JZ, Zhang L, et al: Hepatocellular carcinoma associated microRNA expression signature: Integrated bioinformatics analysis, experimental validation and clinical significance. Oncotarget. 6:25093–25108. 2015. View Article : Google Scholar : PubMed/NCBI
5	Li Z, Yu X, Shen J, Wu WK and Chan MT: MicroRNA expression and its clinical implications in Ewing's sarcoma. Cell Prolif. 48:1–6. 2015. View Article : Google Scholar
6	Lee HK, Finniss S, Cazacu S, Bucris E, Ziv-Av A, Xiang C, Bobbitt K, Rempel SA, Hasselbach L, Mikkelsen T, et al: Mesenchymal stem cells deliver synthetic microRNA mimics to glioma cells and glioma stem cells and inhibit their cell migration and self-renewal. Oncotarget. 4:346–361. 2013. View Article : Google Scholar : PubMed/NCBI
7	Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI
8	Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI
9	Pichler M and Calin GA: MicroRNAs in cancer: From developmental genes in worms to their clinical application in patients. Br J Cancer. 113:569–573. 2015. View Article : Google Scholar : PubMed/NCBI
10	Wang W, Zhang E and Lin C: MicroRNAs in tumor angiogenesis. Life Sci. 136:28–35. 2015. View Article : Google Scholar : PubMed/NCBI
11	Kohlhapp FJ, Mitra AK, Lengyel E and Peter ME: MicroRNAs as mediators and communicators between cancer cells and the tumor microenvironment. Oncogene. 34:5857–5868. 2015. View Article : Google Scholar : PubMed/NCBI
12	Zhao D, Jia P, Wang W and Zhang G: VEGF-mediated suppression of cell proliferation and invasion by miR-410 in osteosarcoma. Mol Cell Biochem. 400:87–95. 2015. View Article : Google Scholar
13	Wang Y, Fu J, Jiang M, Zhang X, Cheng L, Xu X, Fan Z, Zhang J, Ye Q and Song H: miR-410 is overexpressed in liver and colorectal tumors and enhances tumor cell growth by silencing FHL1 via a direct/indirect mechanism. PLoS One. 9:e1087082014. View Article : Google Scholar : PubMed/NCBI
14	Chen L, Zhang J, Feng Y, Li R, Sun X, Du W, Piao X, Wang H, Yang D, Sun Y, et al: miR-410 regulates MET to influence the proliferation and invasion of glioma. Int J Biochem Cell Biol. 44:1711–1717. 2012. View Article : Google Scholar : PubMed/NCBI
15	Li D, Yang Y, Zhu G, Liu X, Zhao M, Li X and Yang Q: MicroRNA-410 promotes cell proliferation by targeting BRD7 in non-small cell lung cancer. FEBS Lett. 589:2218–2223. 2015. View Article : Google Scholar : PubMed/NCBI
16	Guo R, Gu J, Zhang Z, Wang Y and Gu C: MicroRNA-410 functions as a tumor suppressor by targeting angiotensin II type 1 receptor in pancreatic cancer. IUBMB Life. 67:42–53. 2015. View Article : Google Scholar : PubMed/NCBI
17	Shen J, Niu W, Zhou M and Zhang H, Ma J, Wang L and Zhang H: MicroRNA-410 suppresses migration and invasion by targeting MDM2 in gastric cancer. PLoS One. 9:e1045102014. View Article : Google Scholar : PubMed/NCBI
18	Chen N, Wang J, Hu Y, Cui B, Li W, Xu G, Liu L and Liu S: MicroRNA-410 reduces the expression of vascular endothelial growth factor and inhibits oxygen-induced retinal neovascularization. PLoS One. 9:e956652014. View Article : Google Scholar : PubMed/NCBI
19	Müssnich P, Raverot G, Jaffrain-Rea ML, Fraggetta F, Wierinckx A, Trouillas J, Fusco A and D'Angelo D: Downregulation of miR-410 targeting the cyclin B1 gene plays a role in pituitary gonadotroph tumors. Cell Cycle. 14:2590–2597. 2015. View Article : Google Scholar : PubMed/NCBI
20	Yu Y, Zhao Y, Sun XH, Ge J, Zhang B, Wang X and Cao XC: Down-regulation of miR-129-5p via the Twist1-Snail feedback loop stimulates the epithelial-mesenchymal transition and is associated with poor prognosis in breast cancer. Oncotarget. 6:34423–34436. 2015.PubMed/NCBI
21	Yu X and Li Z: MicroRNA expression and its implications for diagnosis and therapy of tongue squamous cell carcinoma. J Cell Mol Med. 20:10–16. 2016. View Article : Google Scholar
22	Li Z, Yu X, Shen J, Law PT, Chan MT and Wu WK: MicroRNA expression and its implications for diagnosis and therapy of gallbladder cancer. Oncotarget. 6:13914–13921. 2015. View Article : Google Scholar : PubMed/NCBI
23	Yu X and Li Z: The role of microRNAs expression in laryngeal cancer. Oncotarget. 6:23297–23305. 2015. View Article : Google Scholar : PubMed/NCBI
24	Takahashi RU, Miyazaki H and Ochiya T: The roles of microRNAs in breast cancer. Cancers (Basel). 7:598–616. 2015. View Article : Google Scholar
25	Goh JN, Loo SY, Datta A, Siveen KS, Yap WN, Cai W, Shin EM, Wang C, Kim JE, Chan M, et al: microRNAs in breast cancer: Regulatory roles governing the hallmarks of cancer. Biol Rev Camb Philos Soc. Jan 28–2015.Epub ahead of print. PubMed/NCBI
26	Bracken CP, Khew-Goodall Y and Goodall GJ: Network-based approaches to understand the roles of miR-200 and other microRNAs in cancer. Cancer Res. 75:2594–2599. 2015. View Article : Google Scholar : PubMed/NCBI
27	Huang J, Zhang SY, Gao YM, Liu YF, Liu YB, Zhao ZG and Yang K: MicroRNAs as oncogenes or tumour suppressors in oesophageal cancer: Potential biomarkers and therapeutic targets. Cell Prolif. 47:277–286. 2014. View Article : Google Scholar : PubMed/NCBI
28	Gattolliat CH, Thomas L, Ciafrè SA, Meurice G, Le Teuff G, Job B, Richon C, Combaret V, Dessen P, Valteau-Couanet D, et al: Expression of miR-487b and miR-410 encoded by 14q32.31 locus is a prognostic marker in neuroblastoma. Br J Cancer. 105:1352–1361. 2011. View Article : Google Scholar : PubMed/NCBI
29	Kotiyal S and Bhattacharya S: Breast cancer stem cells, EMT and therapeutic targets. Biochem Biophys Res Commun. 453:112–116. 2014. View Article : Google Scholar : PubMed/NCBI
30	Saitoh M: Epithelial-mesenchymal transition is regulated at post-transcriptional levels by transforming growth factor-β signaling during tumor progression. Cancer Sci. 106:481–488. 2015. View Article : Google Scholar : PubMed/NCBI
31	Kwon CH, Park HJ, Choi JH, Lee JR, Kim HK, Jo HJ, Kim HS, Oh N, Song GA and Park Y: Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer. Oncotarget. 6:20312–20326. 2015. View Article : Google Scholar : PubMed/NCBI
32	Cho HJ, Park SM, Kim IK, Nam IK, Baek KE, Im MJ, Yoo JM, Park SH, Ryu KJ, Han HT, et al: RhoGDI2 promotes epithelial-mesenchymal transition via induction of Snail in gastric cancer cells. Oncotarget. 5:1554–1564. 2014. View Article : Google Scholar : PubMed/NCBI
33	Cichon MA and Radisky DC: ROS-induced epithelial-mesenchymal transition in mammary epithelial cells is mediated by NF-κB-dependent activation of Snail. Oncotarget. 5:2827–2838. 2014. View Article : Google Scholar : PubMed/NCBI
34	Pilli VS, Gupta K, Kotha BP and Aradhyam GK: Snail-mediated Cripto-1 repression regulates the cell cycle and epithelial-mesenchymal transition-related gene expression. FEBS Lett. 589:1249–1256. 2015. View Article : Google Scholar : PubMed/NCBI