Open Access

A new non-muscle-invasive bladder tumor-homing peptide identified by phage display in vivo

  • Authors:
    • Xiaofeng Yang
    • Fan Zhang
    • Junqian Luo
    • Jianzhi Pang
    • Sanhua Yan
    • Fang Luo
    • Jiehao Liu
    • Wei Wang
    • Yongping Cui
    • Xixi Su
  • View Affiliations

  • Published online on: May 23, 2016     https://doi.org/10.3892/or.2016.4829
  • Pages: 79-89
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bladder cancer is common and widespread, and its incidence is increasing. Many new diagnostic methods combined with state-of-the-art technology have been introduced in cystoscopy to collect real-time images of the bladder mucosa for diagnosis, but often miss inconspicuous early-stage tumors. Fluorophore-labeled peptides with high sensitivity and specificity for cancer would be a desirable tool for the detection and treatment of tiny or residual bladder tumors. Phage display and the human non-muscle-invasive bladder cancer cell line BIU-87 were used to identify a peptide. The isolated phage display peptide (CSSPIGRHC, named NYZL1) was tested in vitro for its binding specificity and affinity. Accumulation into xenograft tumors in a nude mouse model was analyzed with FITC-labeled NYZL1. NYZL1, with strong tumor‑homing ability, was identified by in vivo phage library selection in the bladder cancer model. The NYZL1 phage and synthetic FITC-labeled NYZL1 peptides bound to tumor tissues and cells, but were hardly detected in normal control organs. Notably, accumulation of FITC-NYZL1 in bladder tumor cells was time-dependent. Biodistribution studies of xenografts of BIU-87 cells showed accumulation of injected FITC-NYZL1 in the tumors, and the bound peptide could not be removed by perfusion after 24 h. The mouse model of bladder tumor showed increased fluorescence intensity in the tumor-bearing bladder in comparison with normal bladder tissues after 4-6 h. In conclusion, NYZL1 may represent a lead peptide structure applicable in the development of optical molecular imaging.
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July-2016
Volume 36 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Yang X, Zhang F, Luo J, Pang J, Yan S, Luo F, Liu J, Wang W, Cui Y, Su X, Su X, et al: A new non-muscle-invasive bladder tumor-homing peptide identified by phage display in vivo . Oncol Rep 36: 79-89, 2016
APA
Yang, X., Zhang, F., Luo, J., Pang, J., Yan, S., Luo, F. ... Su, X. (2016). A new non-muscle-invasive bladder tumor-homing peptide identified by phage display in vivo . Oncology Reports, 36, 79-89. https://doi.org/10.3892/or.2016.4829
MLA
Yang, X., Zhang, F., Luo, J., Pang, J., Yan, S., Luo, F., Liu, J., Wang, W., Cui, Y., Su, X."A new non-muscle-invasive bladder tumor-homing peptide identified by phage display in vivo ". Oncology Reports 36.1 (2016): 79-89.
Chicago
Yang, X., Zhang, F., Luo, J., Pang, J., Yan, S., Luo, F., Liu, J., Wang, W., Cui, Y., Su, X."A new non-muscle-invasive bladder tumor-homing peptide identified by phage display in vivo ". Oncology Reports 36, no. 1 (2016): 79-89. https://doi.org/10.3892/or.2016.4829