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Article

Synaptotagmin-like protein 2 gene promotes the metastatic potential in ovarian cancer

  • Authors:
    • Hye Youn Sung
    • Jihye Han
    • Woong Ju
    • Jung-Hyuck Ahn
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, School of Medicine, Ewha Womans University, Yangcheon-gu, Seoul 158-710, Republic of Korea, Department of Obstetrics and Gynecology, School of Medicine, Ewha Womans University, Yangcheon-gu, Seoul 158-710, Republic of Korea
  • Pages: 535-541
    |
    Published online on: May 24, 2016
       https://doi.org/10.3892/or.2016.4835
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Abstract

Ovarian cancer (OC) metastasis has unique biological behavior and most commonly occurs via the transcoelomic route. Previously, we established a mouse xenograft model of human ovarian carcinoma and analyzed alterations in gene expression during metastasis. Among the genes that were differentially expressed more than 2-fold in the xenografts compared with the SK-OV-3 cells, we selected synaptotagmin-like protein 2 (SYTL2) and investigated the mechanisms regulating its expression and its gene function in OC. The mRNA expression of SYTL2 was significantly upregulated and the methylation of specific CpG sites within the SYTL2 promoter was decreased in the metastatic implants from the ovarian carcinoma xenografts compared to wild-type SK-OV-3 cells. Treatment with the demethylating agent 5-aza‑2'-deoxycytidine and/or the histone deacetylase inhibitor Trichostatin A induced upregulation of SYTL2 in SK-OV-3 cells, implying that a DNA methylation-dependent epigenetic mechanism is involved in the regulation of SYTL2 expression. We also found that overexpression of SYTL2 promoted metastatic potential, including increased migration and invasiveness in the ovarian carcinoma cells. Furthermore, we utilized publicly available gene expression data to confirm the correlation between SYTL2 expression and poor prognosis in serous-type OC patients. Our findings provide novel evidence for the direct association of SYTL2 with the metastatic potential of ovarian carcinoma cells and its influence on metastatic recurrence of OC.
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Copy and paste a formatted citation
Spandidos Publications style
Sung HY, Han J, Ju W and Ahn J: Synaptotagmin-like protein 2 gene promotes the metastatic potential in ovarian cancer. Oncol Rep 36: 535-541, 2016.
APA
Sung, H.Y., Han, J., Ju, W., & Ahn, J. (2016). Synaptotagmin-like protein 2 gene promotes the metastatic potential in ovarian cancer. Oncology Reports, 36, 535-541. https://doi.org/10.3892/or.2016.4835
MLA
Sung, H. Y., Han, J., Ju, W., Ahn, J."Synaptotagmin-like protein 2 gene promotes the metastatic potential in ovarian cancer". Oncology Reports 36.1 (2016): 535-541.
Chicago
Sung, H. Y., Han, J., Ju, W., Ahn, J."Synaptotagmin-like protein 2 gene promotes the metastatic potential in ovarian cancer". Oncology Reports 36, no. 1 (2016): 535-541. https://doi.org/10.3892/or.2016.4835
Copy and paste a formatted citation
x
Spandidos Publications style
Sung HY, Han J, Ju W and Ahn J: Synaptotagmin-like protein 2 gene promotes the metastatic potential in ovarian cancer. Oncol Rep 36: 535-541, 2016.
APA
Sung, H.Y., Han, J., Ju, W., & Ahn, J. (2016). Synaptotagmin-like protein 2 gene promotes the metastatic potential in ovarian cancer. Oncology Reports, 36, 535-541. https://doi.org/10.3892/or.2016.4835
MLA
Sung, H. Y., Han, J., Ju, W., Ahn, J."Synaptotagmin-like protein 2 gene promotes the metastatic potential in ovarian cancer". Oncology Reports 36.1 (2016): 535-541.
Chicago
Sung, H. Y., Han, J., Ju, W., Ahn, J."Synaptotagmin-like protein 2 gene promotes the metastatic potential in ovarian cancer". Oncology Reports 36, no. 1 (2016): 535-541. https://doi.org/10.3892/or.2016.4835
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