Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells

  • Authors:
    • See-Hyoung Park
    • Young II Yoon
    • Hyoungwon Moon
    • Ga-Hyun Lee
    • Byung-Heon Lee
    • Tae-Jong Yoon
    • Hak Jong Lee
  • View Affiliations

  • Published online on: May 24, 2016     https://doi.org/10.3892/or.2016.4836
  • Pages: 131-136
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Abstract

Gas (SF6)-filled microbubbles (MBs) were prepared by emulsion and solvent-evaporation method. The prepared MBs were further conjugated with doxorubicin (Dox)-loaded nano-sized liposome and peptide ligands to interleukin-4 receptor (IL4R) for targeting brain tumor cells. The final MB-liposome (Dox)-IL4R targeting peptide ligand [MB-Lipo (Dox)-IL4RTP] had a spherical structure with the mean size of 1,500 nm. The MB-Lipo (Dox)‑IL4RTP exhibited cellular uptake in U87MG brain tumor cells (a brain tumor cell line expressing strongly IL4R) with frequency ultrasound energy suggesting that MB-Lipo (Dox)‑IL4RTP provided effective targeting ability for brain tumor cells. In addition, WST-1 assay results showed that MB-Lipo (Dox)‑IL4RTP inhibited the proliferation of U87MG cells IL4R‑dependently. This was confirmed by western blotting of γH2AX, phospho (Ser15)-p53, p53 and p21 which are signal transduction proteins involved in DNA damage response and cell cycle arrest. Taken together, these results indicate that MB-Lipo (Dox)-IL4RTP represents a promising ultrasonic contrast agent for tumor-targeting ultrasonic imaging.
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July-2016
Volume 36 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Park S, Yoon YI, Moon H, Lee G, Lee B, Yoon T and Lee HJ: Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells. Oncol Rep 36: 131-136, 2016
APA
Park, S., Yoon, Y.I., Moon, H., Lee, G., Lee, B., Yoon, T., & Lee, H.J. (2016). Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells. Oncology Reports, 36, 131-136. https://doi.org/10.3892/or.2016.4836
MLA
Park, S., Yoon, Y. I., Moon, H., Lee, G., Lee, B., Yoon, T., Lee, H. J."Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells". Oncology Reports 36.1 (2016): 131-136.
Chicago
Park, S., Yoon, Y. I., Moon, H., Lee, G., Lee, B., Yoon, T., Lee, H. J."Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells". Oncology Reports 36, no. 1 (2016): 131-136. https://doi.org/10.3892/or.2016.4836