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Article

Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells

  • Authors:
    • See-Hyoung Park
    • Young II Yoon
    • Hyoungwon Moon
    • Ga-Hyun Lee
    • Byung-Heon Lee
    • Tae-Jong Yoon
    • Hak Jong Lee
  • View Affiliations / Copyright

    Affiliations: Program in Nano Science and Technology, Department of Transdisciplinary Studies, Seoul National University, Graduate School of Convergence Science and Technology, Suwon, Republic of Korea, Department of Radiology, Seoul National University, Bundang Hospital, Seungnam, Republic of Korea, Department of Cellular and Molecular Biology, California State University, Chico, CA, USA, Department of Biochemistry and Cell Biology, Kyungpook National University, Daegu, Republic of Korea, Department of Pharmacy, Ajou University, Suwon, Republic of Korea
  • Pages: 131-136
    |
    Published online on: May 24, 2016
       https://doi.org/10.3892/or.2016.4836
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Abstract

Gas (SF6)-filled microbubbles (MBs) were prepared by emulsion and solvent-evaporation method. The prepared MBs were further conjugated with doxorubicin (Dox)-loaded nano-sized liposome and peptide ligands to interleukin-4 receptor (IL4R) for targeting brain tumor cells. The final MB-liposome (Dox)-IL4R targeting peptide ligand [MB-Lipo (Dox)-IL4RTP] had a spherical structure with the mean size of 1,500 nm. The MB-Lipo (Dox)‑IL4RTP exhibited cellular uptake in U87MG brain tumor cells (a brain tumor cell line expressing strongly IL4R) with frequency ultrasound energy suggesting that MB-Lipo (Dox)‑IL4RTP provided effective targeting ability for brain tumor cells. In addition, WST-1 assay results showed that MB-Lipo (Dox)‑IL4RTP inhibited the proliferation of U87MG cells IL4R‑dependently. This was confirmed by western blotting of γH2AX, phospho (Ser15)-p53, p53 and p21 which are signal transduction proteins involved in DNA damage response and cell cycle arrest. Taken together, these results indicate that MB-Lipo (Dox)-IL4RTP represents a promising ultrasonic contrast agent for tumor-targeting ultrasonic imaging.
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Copy and paste a formatted citation
Spandidos Publications style
Park S, Yoon YI, Moon H, Lee G, Lee B, Yoon T and Lee HJ: Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells. Oncol Rep 36: 131-136, 2016.
APA
Park, S., Yoon, Y.I., Moon, H., Lee, G., Lee, B., Yoon, T., & Lee, H.J. (2016). Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells. Oncology Reports, 36, 131-136. https://doi.org/10.3892/or.2016.4836
MLA
Park, S., Yoon, Y. I., Moon, H., Lee, G., Lee, B., Yoon, T., Lee, H. J."Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells". Oncology Reports 36.1 (2016): 131-136.
Chicago
Park, S., Yoon, Y. I., Moon, H., Lee, G., Lee, B., Yoon, T., Lee, H. J."Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells". Oncology Reports 36, no. 1 (2016): 131-136. https://doi.org/10.3892/or.2016.4836
Copy and paste a formatted citation
x
Spandidos Publications style
Park S, Yoon YI, Moon H, Lee G, Lee B, Yoon T and Lee HJ: Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells. Oncol Rep 36: 131-136, 2016.
APA
Park, S., Yoon, Y.I., Moon, H., Lee, G., Lee, B., Yoon, T., & Lee, H.J. (2016). Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells. Oncology Reports, 36, 131-136. https://doi.org/10.3892/or.2016.4836
MLA
Park, S., Yoon, Y. I., Moon, H., Lee, G., Lee, B., Yoon, T., Lee, H. J."Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells". Oncology Reports 36.1 (2016): 131-136.
Chicago
Park, S., Yoon, Y. I., Moon, H., Lee, G., Lee, B., Yoon, T., Lee, H. J."Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells". Oncology Reports 36, no. 1 (2016): 131-136. https://doi.org/10.3892/or.2016.4836
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