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Article

Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway

  • Authors:
    • Weiping Zhu
    • Dihua Zhan
    • Lu Wang
    • Dening Ma
    • Mingrong Cheng
    • Huipeng Wang
    • Jiaying Zhao
    • Yuankun Cai
    • Zhijian Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Hepatic Surgery, Fudan University Shanghai Cancer Center, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China, Department of Liver Surgery, The Affiliated Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China, Department of General Surgery, Pudong New Area District Zhoupu Hospital, Shanghai 201318, P.R. China, Department of General Surgery, The Affiliated Shanghai Fifth People's Hospital of Fudan University, Shanghai 200240, P.R. China
  • Pages: 845-852
    |
    Published online on: May 27, 2016
       https://doi.org/10.3892/or.2016.4839
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Abstract

TRAIL is a tumor-selective apoptosis-inducing cytokine playing a vital role in the surveillance and elimination of some tumor cells. However, some tumors are resistant to TRAIL treatment. Proteasome inhibitor MG132 exhibits anti-proliferative and pro-apoptotic properties in many tumors. In this study, we demonstrated that proteasome inhibitor MG132 in vitro and in vivo potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells. MG132 was able to inhibit the proliferation of GBC-SD cells and induce apoptosis in a dose-dependent manner. The induction of apoptosis by proteasome inhibitor MG132 was mainly through the extrinsic apoptotic pathways of caspase activation such as caspase-8, caspase-3 and PARP cleavage. In addition, this process was also dependent on the upregulation of death receptor 5 (DR5), which promoted TRAIL-induced apoptosis in GBC-SD cells. Taken together, these findings indicate that MG132 possesses anti-gallbladder cancer potential that correlate with regulation of DR5-dependent pathway, and suggest that MG132 may be a promising agent for sensitizing GBC-SD cells to TRAIL-induced apoptosis.
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1 

Bizama C, García P, Espinoza JA, Weber H, Leal P, Nervi B and Roa JC: Targeting specific molecular pathways holds promise for advanced gallbladder cancer therapy. Cancer Treat Rev. 41:222–234. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Misra S, Chaturvedi A, Misra NC and Sharma ID: Carcinoma of the gallbladder. Lancet Oncol. 4:167–176. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Henley SJ, Weir HK, Jim MA, Watson M and Richardson LC: Gallbladder cancer incidence and mortality, United States 1999–2011. Cancer Epidemiol Biomarkers Prev. 24:1319–1326. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Chan E and Berlin J: Biliary tract cancers: Understudied and poorly understood. J Clin Oncol. 33:1845–1848. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Walczak H, Miller RE, Ariail K, Gliniak B, Griffith TS, Kubin M, Chin W, Jones J, Woodward A, Le T, et al: Tumoricidal activity of tumor necrosis factor-related apoptosis-inducing ligand in vivo. Nat Med. 5:157–163. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Nesterov A, Nikrad M, Johnson T and Kraft AS: Oncogenic Ras sensitizes normal human cells to tumor necrosis factor-alpha-related apoptosis-inducing ligand-induced apoptosis. Cancer Res. 64:3922–3927. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Pan G, O'Rourke K, Chinnaiyan AM, Gentz R, Ebner R, Ni J and Dixit VM: The receptor for the cytotoxic ligand TRAIL. Science. 276:111–113. 1997. View Article : Google Scholar : PubMed/NCBI

8 

Bellail AC, Tse MC, Song JH, Phuphanich S, Olson JJ, Sun SY and Hao C: DR5-mediated DISC controls caspase-8 cleavage and initiation of apoptosis in human glioblastomas. J Cell Mol Med. 14A:1303–1317. 2010. View Article : Google Scholar

9 

Fernald K and Kurokawa M: Evading apoptosis in cancer. Trends Cell Biol. 23:620–633. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Shankar S and Srivastava RK: Enhancement of therapeutic potential of TRAIL by cancer chemotherapy and irradiation: mechanisms and clinical implications. Drug Resist Updat. 7:139–156. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Shin EA, Sohn EJ, Won G, Choi JU, Jeong M, Kim B, Kim MJ and Kim SH: Upregulation of microRNA135a-3p and death receptor 5 plays a critical role in Tanshinone I sensitized prostate cancer cells to TRAIL induced apoptosis. Oncotarget. 5:5624–5636. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Stuckey DW and Shah K: TRAIL on trial: Preclinical advances in cancer therapy. Trends Mol Med. 19:685–694. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Sarhan D, Wennerberg E, D'Arcy P, Gurajada D, Linder S and Lundqvist A: A novel inhibitor of proteasome deubiquitinating activity renders tumor cells sensitive to TRAIL-mediated apoptosis by natural killer cells and T cells. Cancer Immunol Immunother. 62:1359–1368. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Dokouhaki P, Schuh NW, Joe B, Allen CA, Der SD, Tsao MS and Zhang L: NKG2D regulates production of soluble TRAIL by ex vivo expanded human γδ T cells. Eur J Immunol. 43:3175–3182. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Nalepa G, Rolfe M and Harper JW: Drug discovery in the ubiquitin-proteasome system. Nat Rev Drug Discov. 5:596–613. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Adams J: The proteasome: A suitable antineoplastic target. Nat Rev Cancer. 4:349–360. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Johnson DE: The ubiquitin-proteasome system: Opportunities for therapeutic intervention in solid tumors. Endocr Relat Cancer. 22:T1–T17. 2015. View Article : Google Scholar

18 

He Q, Huang Y and Sheikh MS: Proteasome inhibitor MG132 upregulates death receptor 5 and cooperates with Apo2L/TRAIL to induce apoptosis in Bax-proficient and -deficient cells. Oncogene. 23:2554–2558. 2004. View Article : Google Scholar

19 

Pandit B and Gartel AL: Proteasome inhibitors induce p53-independent apoptosis in human cancer cells. Am J Pathol. 178:355–360. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Ustundag Y, Bronk SF and Gores GJ: Proteasome inhibition-induces endoplasmic reticulum dysfunction and cell death of human cholangiocarcinoma cells. World J Gastroenterol. 13:851–857. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Kim OH, Lim JH, Woo KJ, Kim YH, Jin IN, Han ST, Park JW and Kwon TK: Influence of p53 and p21Waf1 expression on G2/M phase arrest of colorectal carcinoma HCT116 cells to proteasome inhibitors. Int J Oncol. 24:935–941. 2004.PubMed/NCBI

22 

Watts TH: TNF/TNFR family members in costimulation of T cell responses. Annu Rev Immunol. 23:23–68. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Vucic D, Dixit VM and Wertz IE: Ubiquitylation in apoptosis: A post-translational modification at the edge of life and death. Nat Rev Mol Cell Biol. 12:439–452. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Song JJ, Szczepanski MJ, Kim SY, Kim JH, An JY, Kwon YT, Alcala MA Jr, Bartlett DL and Lee YJ: c-Cbl-mediated degradation of TRAIL receptors is responsible for the development of the early phase of TRAIL resistance. Cell Signal. 22:553–563. 2010. View Article : Google Scholar :

25 

Li X, Huang T, Jiang G, Gong W, Qian H and Zou C: Proteasome inhibitor MG132 enhances TRAIL-induced apoptosis and inhibits invasion of human osteosarcoma OS732 cells. Biochem Biophys Res Commun. 439:179–186. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Han B, Yao W, Oh YT, Tong JS, Li S, Deng J, Yue P, Khuri FR and Sun SY: The novel proteasome inhibitor carfilzomib activates and enhances extrinsic apoptosis involving stabilization of death receptor 5. Oncotarget. 6:17532–17542. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Ko H, Jeong MH, Jeon H, Sung GJ, So Y, Kim I, Son J, Lee SW, Yoon HG and Choi KC: Delphinidin sensitizes prostate cancer cells to TRAIL-induced apoptosis, by inducing DR5 and causing caspase-mediated HDAC3 cleavage. Oncotarget. 6:9970–9984. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Kelley SK and Ashkenazi A: Targeting death receptors in cancer with Apo2L/TRAIL. Curr Opin Pharmacol. 4:333–339. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Merchant MS, Geller JI, Baird K, Chou AJ, Galli S, Charles A, Amaoko M, Rhee EH, Price A, Wexler LH, et al: Phase I trial and pharmacokinetic study of lexatumumab in pediatric patients with solid tumors. J Clin Oncol. 30:4141–4147. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Jazirehi AR, Kurdistani SK and Economou JS: Histone deacetylase inhibitor sensitizes apoptosis-resistant melanomas to cytotoxic human T lymphocytes through regulation of TRAIL/DR5 pathway. J Immunol. 192:3981–3989. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Lee YJ, Seol JW, Jeong JK, Moon MH and Park SY: Inhibition of the ubiquitin-proteasome system sensitizes TRAIL-resistant prostate cancer cells by up-regulation of death receptor 5. Mol Med Rep. 4:1255–1259. 2011.PubMed/NCBI

32 

de Wilt LH, Kroon J, Jansen G, de Jong S, Peters GJ and Kruyt FA: Bortezomib and TRAIL: A perfect match for apoptotic elimination of tumour cells? Crit Rev Oncol Hematol. 85:363–372. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhu W, Zhan D, Wang L, Ma D, Cheng M, Wang H, Zhao J, Cai Y and Cheng Z: Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway. Oncol Rep 36: 845-852, 2016.
APA
Zhu, W., Zhan, D., Wang, L., Ma, D., Cheng, M., Wang, H. ... Cheng, Z. (2016). Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway. Oncology Reports, 36, 845-852. https://doi.org/10.3892/or.2016.4839
MLA
Zhu, W., Zhan, D., Wang, L., Ma, D., Cheng, M., Wang, H., Zhao, J., Cai, Y., Cheng, Z."Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway". Oncology Reports 36.2 (2016): 845-852.
Chicago
Zhu, W., Zhan, D., Wang, L., Ma, D., Cheng, M., Wang, H., Zhao, J., Cai, Y., Cheng, Z."Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway". Oncology Reports 36, no. 2 (2016): 845-852. https://doi.org/10.3892/or.2016.4839
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu W, Zhan D, Wang L, Ma D, Cheng M, Wang H, Zhao J, Cai Y and Cheng Z: Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway. Oncol Rep 36: 845-852, 2016.
APA
Zhu, W., Zhan, D., Wang, L., Ma, D., Cheng, M., Wang, H. ... Cheng, Z. (2016). Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway. Oncology Reports, 36, 845-852. https://doi.org/10.3892/or.2016.4839
MLA
Zhu, W., Zhan, D., Wang, L., Ma, D., Cheng, M., Wang, H., Zhao, J., Cai, Y., Cheng, Z."Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway". Oncology Reports 36.2 (2016): 845-852.
Chicago
Zhu, W., Zhan, D., Wang, L., Ma, D., Cheng, M., Wang, H., Zhao, J., Cai, Y., Cheng, Z."Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway". Oncology Reports 36, no. 2 (2016): 845-852. https://doi.org/10.3892/or.2016.4839
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