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Article

7-Difluoromethoxyl-5,4'-di-n-octyl genistein inhibits the stem-like characteristics of gastric cancer stem-like cells and reverses the phenotype of epithelial-mesenchymal transition in gastric cancer cells

Corrigendum in: /10.3892/or.2022.8391
  • Authors:
    • Xiaozheng Cao
    • Kaiqun Ren
    • Zhengwei Song
    • Duo Li
    • Meifang Quan
    • Yu Zheng
    • Jianguo Cao
    • Wenbin Zeng
    • Hui Zou
  • View Affiliations / Copyright

    Affiliations: Medical College, Hunan Normal University, Changsha, Hunan 410013, P.R. China, School of Pharmaceutical Sciences and Molecular Imaging Research Center, Central South University, Changsha, Hunan 410013, P.R. China
  • Pages: 1157-1165
    |
    Published online on: June 2, 2016
       https://doi.org/10.3892/or.2016.4848
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Abstract

7-Difluoromethoxyl-5,4'-di-n-octyl genistein (DFOG), a novel synthetic genistein analogue, exerts anticarcinogenic activity in several types of cancers, including gastric cancer. Accumulating evidence in recent years strongly indicates the existence of cancer stem cells in gastric cancer. The objective of the present study was to investigate whether DFOG inhibits the stemness and reverses the epithelial-mesenchymal transition (EMT) phenotype of gastric cancer stem-like cells (GCSLCs) derived from human gastric cancer SGC-7901 cells and to identify its potential mechanism. Sphere-forming cells (SFCs) from the SGC-7901 cells possessed the properties of GCSLCs. DFOG preferentially inhibited self-renewal, cell migration and cell invasion, and downregulated the expression of stem cell biomarkers in a dose-dependent manner. At the molecular level, these effects were accompanied by the downregulation of forkhead box M1 (FoxM1). Meanwhile, FoxM1 siRNA transfection was able to synergize the inhibition of expression of FoxM1 and Twist1 induced by DFOG in GCSLCs. In addition, we found that DFOG treatment decreased the expression of N-cadherin and increased the expression of E-cadherin. More importantly, FoxM1 siRNA transfection cooperated with DFOG to suppress the self-renewal capacity, cell migration and cell invasion, and downregulated the expression of CD133, CD44, ALDH1, and also regulated the expression of N-cadherin and E-cadherin. These findings showed that DFOG inhibited the stem-like characteristics of GCSLCs and reversed the EMT phenotype by modulation of FoxM1 and further decreased Twist1 expression. Our results provide a further rationale and experimental basis for using DFOG to improve the efficacy of treatment for patients with gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Cao X, Ren K, Song Z, Li D, Quan M, Zheng Y, Cao J, Zeng W and Zou H: 7-Difluoromethoxyl-5,4'-di-n-octyl genistein inhibits the stem-like characteristics of gastric cancer stem-like cells and reverses the phenotype of epithelial-mesenchymal transition in gastric cancer cells Corrigendum in /10.3892/or.2022.8391. Oncol Rep 36: 1157-1165, 2016.
APA
Cao, X., Ren, K., Song, Z., Li, D., Quan, M., Zheng, Y. ... Zou, H. (2016). 7-Difluoromethoxyl-5,4'-di-n-octyl genistein inhibits the stem-like characteristics of gastric cancer stem-like cells and reverses the phenotype of epithelial-mesenchymal transition in gastric cancer cells Corrigendum in /10.3892/or.2022.8391. Oncology Reports, 36, 1157-1165. https://doi.org/10.3892/or.2016.4848
MLA
Cao, X., Ren, K., Song, Z., Li, D., Quan, M., Zheng, Y., Cao, J., Zeng, W., Zou, H."7-Difluoromethoxyl-5,4'-di-n-octyl genistein inhibits the stem-like characteristics of gastric cancer stem-like cells and reverses the phenotype of epithelial-mesenchymal transition in gastric cancer cells Corrigendum in /10.3892/or.2022.8391". Oncology Reports 36.2 (2016): 1157-1165.
Chicago
Cao, X., Ren, K., Song, Z., Li, D., Quan, M., Zheng, Y., Cao, J., Zeng, W., Zou, H."7-Difluoromethoxyl-5,4'-di-n-octyl genistein inhibits the stem-like characteristics of gastric cancer stem-like cells and reverses the phenotype of epithelial-mesenchymal transition in gastric cancer cells Corrigendum in /10.3892/or.2022.8391". Oncology Reports 36, no. 2 (2016): 1157-1165. https://doi.org/10.3892/or.2016.4848
Copy and paste a formatted citation
x
Spandidos Publications style
Cao X, Ren K, Song Z, Li D, Quan M, Zheng Y, Cao J, Zeng W and Zou H: 7-Difluoromethoxyl-5,4'-di-n-octyl genistein inhibits the stem-like characteristics of gastric cancer stem-like cells and reverses the phenotype of epithelial-mesenchymal transition in gastric cancer cells Corrigendum in /10.3892/or.2022.8391. Oncol Rep 36: 1157-1165, 2016.
APA
Cao, X., Ren, K., Song, Z., Li, D., Quan, M., Zheng, Y. ... Zou, H. (2016). 7-Difluoromethoxyl-5,4'-di-n-octyl genistein inhibits the stem-like characteristics of gastric cancer stem-like cells and reverses the phenotype of epithelial-mesenchymal transition in gastric cancer cells Corrigendum in /10.3892/or.2022.8391. Oncology Reports, 36, 1157-1165. https://doi.org/10.3892/or.2016.4848
MLA
Cao, X., Ren, K., Song, Z., Li, D., Quan, M., Zheng, Y., Cao, J., Zeng, W., Zou, H."7-Difluoromethoxyl-5,4'-di-n-octyl genistein inhibits the stem-like characteristics of gastric cancer stem-like cells and reverses the phenotype of epithelial-mesenchymal transition in gastric cancer cells Corrigendum in /10.3892/or.2022.8391". Oncology Reports 36.2 (2016): 1157-1165.
Chicago
Cao, X., Ren, K., Song, Z., Li, D., Quan, M., Zheng, Y., Cao, J., Zeng, W., Zou, H."7-Difluoromethoxyl-5,4'-di-n-octyl genistein inhibits the stem-like characteristics of gastric cancer stem-like cells and reverses the phenotype of epithelial-mesenchymal transition in gastric cancer cells Corrigendum in /10.3892/or.2022.8391". Oncology Reports 36, no. 2 (2016): 1157-1165. https://doi.org/10.3892/or.2016.4848
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