Open Access

Regulation of tumorigenesis in oral epithelial cells by defined reprogramming factors Oct4 and Sox2

  • Authors:
    • Jinghua Cai
    • Baoxia He
    • Xinming Li
    • Minglei Sun
    • Alfred King-Yin Lam
    • Bin Qiao
    • Weiliu Qiu
  • View Affiliations

  • Published online on: June 3, 2016     https://doi.org/10.3892/or.2016.4851
  • Pages: 651-658
  • Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oct4 and Sox2 are pluripotent stem cell factors but the interplay between them in tumorigenesis is unclear. The aim of the present study was to investigate the roles of Oct4 and Sox2 in the reprogramming of oral cancer stem cells. One or both Oct4 and Sox2 were overexpressed in immortalized oral epithelial (hTERT+-OME) cells by lentivirus transduction. In addition, Oct4 and Sox2 proteins in two oral squamous cell carcinoma cell (OSCC) lines (Cal27 and primary cultured OSCC from a T2N2M0 patient) were individually or combinedly knocked down by shRNA. The results showed that the doubly transduced (Oct4+Sox2+) cells could trigger neoplasms in immunodeficient mice after lentivirus transduction, but single transduced (Oct4+ or Sox2+) cells had no tumor formation ability. The knockdown Sox2low and knockdown Oct4lowSox2low cells resulted in decreased tumor size in the immunodeficient mice but the single knockdown Oct4low cancer cells acquired more aggressive xenografts. Our findings suggest that Oct4+Sox2+ cells may be reprogrammed cancer stem cells inducing oral carcinogenesis.
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August-2016
Volume 36 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cai J, He B, Li X, Sun M, Lam AK, Qiao B and Qiu W: Regulation of tumorigenesis in oral epithelial cells by defined reprogramming factors Oct4 and Sox2. Oncol Rep 36: 651-658, 2016.
APA
Cai, J., He, B., Li, X., Sun, M., Lam, A.K., Qiao, B., & Qiu, W. (2016). Regulation of tumorigenesis in oral epithelial cells by defined reprogramming factors Oct4 and Sox2. Oncology Reports, 36, 651-658. https://doi.org/10.3892/or.2016.4851
MLA
Cai, J., He, B., Li, X., Sun, M., Lam, A. K., Qiao, B., Qiu, W."Regulation of tumorigenesis in oral epithelial cells by defined reprogramming factors Oct4 and Sox2". Oncology Reports 36.2 (2016): 651-658.
Chicago
Cai, J., He, B., Li, X., Sun, M., Lam, A. K., Qiao, B., Qiu, W."Regulation of tumorigenesis in oral epithelial cells by defined reprogramming factors Oct4 and Sox2". Oncology Reports 36, no. 2 (2016): 651-658. https://doi.org/10.3892/or.2016.4851