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Article

miR-375 exhibits a more effective tumor-suppressor function in laryngeal squamous carcinoma cells by regulating KLF4 expression compared with simple co-transfection of miR-375 and miR-206

  • Authors:
    • Yan Guo
    • Ran An
    • Rui Zhao
    • Yanan Sun
    • Ming Liu
    • Linli Tian
  • View Affiliations / Copyright

    Affiliations: Service of Head and Neck Surgery, Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Service of Laryngology, Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China
  • Pages: 952-960
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    Published online on: June 3, 2016
       https://doi.org/10.3892/or.2016.4852
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Abstract

MicroRNAs (miRNAs) are reported to be important regulators of cancer-related processes, and function either as oncogenes or as tumor-suppressor genes. It was found that miR-375 was downregulated in samples of laryngeal squamous cell carcinomas (LSCCs) as compared to the level noted in adjacent non-tumor tissues, and it was inversely correlated with T grade, lymph node metastases and clinical tumor stage. Overexpression of miR-375 led to a decreased protein level of Krüppel-like factor 4 (KLF4) and marked suppression of the proliferation and invasion, and induced apoptosis of LSCC cell line Hep-2 using Cell Counting Kit-8, Transwell chamber and cell cycle assays. In addition, we examined the influence of the upregulation of miR-206 alone and upregulation of both miR-375 and miR-206 on the expression of KLF4 and Hep-2 cell behavior. The results showed that compared with the function of miR-375 in tumor suppression by regulating KLF4, co-transfection of miR-375 and miR-206 exhibited a less effective inhibitory effect not only on tumor cell proliferation and invasion, but also on tumor cell apoptosis. Taken together, miR-375 is possibly a tumor suppressor in LSCC by regulating KLF4. In addition, simple overexpression of several miRNAs did not entail higher efficacy than a single miRNA, similar to co-transfecions of miR-375 and miR-206.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Y, An R, Zhao R, Sun Y, Liu M and Tian L: miR-375 exhibits a more effective tumor-suppressor function in laryngeal squamous carcinoma cells by regulating KLF4 expression compared with simple co-transfection of miR-375 and miR-206. Oncol Rep 36: 952-960, 2016.
APA
Guo, Y., An, R., Zhao, R., Sun, Y., Liu, M., & Tian, L. (2016). miR-375 exhibits a more effective tumor-suppressor function in laryngeal squamous carcinoma cells by regulating KLF4 expression compared with simple co-transfection of miR-375 and miR-206. Oncology Reports, 36, 952-960. https://doi.org/10.3892/or.2016.4852
MLA
Guo, Y., An, R., Zhao, R., Sun, Y., Liu, M., Tian, L."miR-375 exhibits a more effective tumor-suppressor function in laryngeal squamous carcinoma cells by regulating KLF4 expression compared with simple co-transfection of miR-375 and miR-206". Oncology Reports 36.2 (2016): 952-960.
Chicago
Guo, Y., An, R., Zhao, R., Sun, Y., Liu, M., Tian, L."miR-375 exhibits a more effective tumor-suppressor function in laryngeal squamous carcinoma cells by regulating KLF4 expression compared with simple co-transfection of miR-375 and miR-206". Oncology Reports 36, no. 2 (2016): 952-960. https://doi.org/10.3892/or.2016.4852
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Y, An R, Zhao R, Sun Y, Liu M and Tian L: miR-375 exhibits a more effective tumor-suppressor function in laryngeal squamous carcinoma cells by regulating KLF4 expression compared with simple co-transfection of miR-375 and miR-206. Oncol Rep 36: 952-960, 2016.
APA
Guo, Y., An, R., Zhao, R., Sun, Y., Liu, M., & Tian, L. (2016). miR-375 exhibits a more effective tumor-suppressor function in laryngeal squamous carcinoma cells by regulating KLF4 expression compared with simple co-transfection of miR-375 and miR-206. Oncology Reports, 36, 952-960. https://doi.org/10.3892/or.2016.4852
MLA
Guo, Y., An, R., Zhao, R., Sun, Y., Liu, M., Tian, L."miR-375 exhibits a more effective tumor-suppressor function in laryngeal squamous carcinoma cells by regulating KLF4 expression compared with simple co-transfection of miR-375 and miR-206". Oncology Reports 36.2 (2016): 952-960.
Chicago
Guo, Y., An, R., Zhao, R., Sun, Y., Liu, M., Tian, L."miR-375 exhibits a more effective tumor-suppressor function in laryngeal squamous carcinoma cells by regulating KLF4 expression compared with simple co-transfection of miR-375 and miR-206". Oncology Reports 36, no. 2 (2016): 952-960. https://doi.org/10.3892/or.2016.4852
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