Activation of the FAK/PI3K pathway is crucial for AURKA-induced epithelial-mesenchymal transition in laryngeal cancer

  • Authors:
    • Liyun Yang
    • Quan Zhou
    • Xuehua Chen
    • Liping Su
    • Bingya Liu
    • Hao Zhang
  • View Affiliations

  • Published online on: June 13, 2016     https://doi.org/10.3892/or.2016.4872
  • Pages: 819-826
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Abstract

Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant tumors, and the main cause of death is metastasis. Overexpression of aurora kinase A (AURKA) plays an important role in the metastasis of LSCC. However, the mechanism by which AURKA promotes the metastasis of LSCC is poorly understood. Recent accumulating evidence indicates that epithelial-mesenchymal transition (EMT) may be one of the mechanisms of tumor metastasis. In the present study, we studied whether AURKA may induce EMT to promote the metastasis of LSCC. CCK-8 and plate colony-formation assays were carried out to show that AURKA significantly promoted the proliferation of Hep2 cells. Immunofluorescence staining and western blotting showed that EMT-related proteins changed in a time-dependent manner along with the alteration of AURKA, with decreased expression of N-cadherin, vimentin and slug and increased expression of E-cadherin. Additionally, downregulation of the expression of AURKA inhibited FAK/PI3K pathway activity. Inhibition of the FAK/PI3K pathway caused less mesenchymal-like characteristics and reduced the mobility, migration and invasion of Hep2 cells. In conclusion, AURKA may induce EMT to promote metastasis via activation of the FAK/PI3K pathway in LSCC. Those regulatory factors may present new diagnostic biomarkers and potential therapeutic targets for LSCC.
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August-2016
Volume 36 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yang L, Zhou Q, Chen X, Su L, Liu B and Zhang H: Activation of the FAK/PI3K pathway is crucial for AURKA-induced epithelial-mesenchymal transition in laryngeal cancer. Oncol Rep 36: 819-826, 2016
APA
Yang, L., Zhou, Q., Chen, X., Su, L., Liu, B., & Zhang, H. (2016). Activation of the FAK/PI3K pathway is crucial for AURKA-induced epithelial-mesenchymal transition in laryngeal cancer. Oncology Reports, 36, 819-826. https://doi.org/10.3892/or.2016.4872
MLA
Yang, L., Zhou, Q., Chen, X., Su, L., Liu, B., Zhang, H."Activation of the FAK/PI3K pathway is crucial for AURKA-induced epithelial-mesenchymal transition in laryngeal cancer". Oncology Reports 36.2 (2016): 819-826.
Chicago
Yang, L., Zhou, Q., Chen, X., Su, L., Liu, B., Zhang, H."Activation of the FAK/PI3K pathway is crucial for AURKA-induced epithelial-mesenchymal transition in laryngeal cancer". Oncology Reports 36, no. 2 (2016): 819-826. https://doi.org/10.3892/or.2016.4872