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Article

DT-13 attenuates human lung cancer metastasis via regulating NMIIA activity under hypoxia condition

  • Authors:
    • Xiao-Hui Wei
    • Sen-Sen Lin
    • Yang Liu
    • Ren-Ping Zhao
    • Ghulam Jilany Khan
    • Hong-Zhi Du
    • Ting-Ting Mao
    • Bo-Yang Yu
    • Rui-Ming Li
    • Sheng-Tao Yuan
    • Li Sun
  • View Affiliations / Copyright

    Affiliations: Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing, P.R. China, Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, Nanjing, P.R. China, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, P.R. China, Tasly Research Institute, Tianjin Tasly Hodling Group Co., Ltd., Tianjin, P.R. China
  • Pages: 991-999
    |
    Published online on: June 16, 2016
       https://doi.org/10.3892/or.2016.4879
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Abstract

Cancer metastasis plays a major role in tumor deterioration. Metastatic processes are known to be regulated by hypoxic microenvironment and non-muscle myosin IIA (NMIIA). DT-13, a bioactive saponin monomer isolated from Ophiopogon japonicus, has been reported to inhibit various cancer metastasis, but whether NMIIA is involved in the anti-metastatic activity of DT-13 under hypoxia remains to be determined. Thus, this study aims to clarify the role of DT-13 in regulating 95D cell metastasis under hypoxic microenvironment and to further investigate whether NMIIA is involved in the anti-metastatic mechanism of DT-13. We found that DT-13 significantly inhibited 95D cells metastasis in vitro and in vivo. Furthermore, hypoxia significantly inhibited the expression of NMIIA and redistributed NMIIA to the cell periphery, whereas DT-13 reversed the hypoxic effects by upregulating the expression of NMIIA. Moreover, DT-13 treatment redistributed NMIIA to the nuclear periphery and reduced the formation of F-actin in 95D cells. In addition, we found that the Raf-ERK1/2 signaling pathway is involved in regulation of NMIIA by DT-13. Collectively, these findings support NMIIA as a target of DT-13 to prevent lung cancer metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Wei X, Lin S, Liu Y, Zhao R, Khan GJ, Du H, Mao T, Yu B, Li R, Yuan S, Yuan S, et al: DT-13 attenuates human lung cancer metastasis via regulating NMIIA activity under hypoxia condition. Oncol Rep 36: 991-999, 2016.
APA
Wei, X., Lin, S., Liu, Y., Zhao, R., Khan, G.J., Du, H. ... Sun, L. (2016). DT-13 attenuates human lung cancer metastasis via regulating NMIIA activity under hypoxia condition. Oncology Reports, 36, 991-999. https://doi.org/10.3892/or.2016.4879
MLA
Wei, X., Lin, S., Liu, Y., Zhao, R., Khan, G. J., Du, H., Mao, T., Yu, B., Li, R., Yuan, S., Sun, L."DT-13 attenuates human lung cancer metastasis via regulating NMIIA activity under hypoxia condition". Oncology Reports 36.2 (2016): 991-999.
Chicago
Wei, X., Lin, S., Liu, Y., Zhao, R., Khan, G. J., Du, H., Mao, T., Yu, B., Li, R., Yuan, S., Sun, L."DT-13 attenuates human lung cancer metastasis via regulating NMIIA activity under hypoxia condition". Oncology Reports 36, no. 2 (2016): 991-999. https://doi.org/10.3892/or.2016.4879
Copy and paste a formatted citation
x
Spandidos Publications style
Wei X, Lin S, Liu Y, Zhao R, Khan GJ, Du H, Mao T, Yu B, Li R, Yuan S, Yuan S, et al: DT-13 attenuates human lung cancer metastasis via regulating NMIIA activity under hypoxia condition. Oncol Rep 36: 991-999, 2016.
APA
Wei, X., Lin, S., Liu, Y., Zhao, R., Khan, G.J., Du, H. ... Sun, L. (2016). DT-13 attenuates human lung cancer metastasis via regulating NMIIA activity under hypoxia condition. Oncology Reports, 36, 991-999. https://doi.org/10.3892/or.2016.4879
MLA
Wei, X., Lin, S., Liu, Y., Zhao, R., Khan, G. J., Du, H., Mao, T., Yu, B., Li, R., Yuan, S., Sun, L."DT-13 attenuates human lung cancer metastasis via regulating NMIIA activity under hypoxia condition". Oncology Reports 36.2 (2016): 991-999.
Chicago
Wei, X., Lin, S., Liu, Y., Zhao, R., Khan, G. J., Du, H., Mao, T., Yu, B., Li, R., Yuan, S., Sun, L."DT-13 attenuates human lung cancer metastasis via regulating NMIIA activity under hypoxia condition". Oncology Reports 36, no. 2 (2016): 991-999. https://doi.org/10.3892/or.2016.4879
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