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Article

Effects of estradiol on VEGF and bFGF by Akt in endometrial cancer cells are mediated through the NF-κB pathway

  • Authors:
    • Jieqing Zhang
    • Honglin Song
    • Yanqiong Lu
    • Haiyan Chen
    • Si Jiang
    • Li Li
  • View Affiliations / Copyright

    Affiliations: Department of Gynecologic Oncology, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
  • Pages: 705-714
    |
    Published online on: June 21, 2016
       https://doi.org/10.3892/or.2016.4888
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Abstract

Endometrial carcinogenesis may be related to the long-term effects of estradiol with no antagonism. However, how estradiol regulates cell proliferation is unknown. In the present study, through investigating the molecular events involved in estradiol induced angiogenics factors VEGF and bFGF, we found that estradiol induced endometrial cancer cell division, proliferation, migratory and invasive capacity in vitro and upregulated mRNA expression and protein synthesis of VEGF and bFGF. The estradiol-dependent induction of the expression of VEGF and bFGF was blocked by ER inhibitor, AKT inhibitor and NF-κB inhibitor (PDTC) in estrogen receptor positive Ishikawa cells and blocked by AKT inhibitor, NF-κB inhibitor (PDTC) in estrogen receptor negative HEC-1A cells. Moreover, estradiol activation of AKT was also blocked by AKT antagonist. NF-κB activation was restricted by estradiol concentration and time. Estradiol leading to VEGF and bFGF induction was also confirmed by the development of xenograft tumors in vivo. Taken together, our data suggest that estradiol induces the production of angiogenic factors via a mechanism involving AKT-mediated NF-κB activation partly in non-genomic manner without the estrogen receptor.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Song H, Lu Y, Chen H, Jiang S and Li L: Effects of estradiol on VEGF and bFGF by Akt in endometrial cancer cells are mediated through the NF-κB pathway. Oncol Rep 36: 705-714, 2016.
APA
Zhang, J., Song, H., Lu, Y., Chen, H., Jiang, S., & Li, L. (2016). Effects of estradiol on VEGF and bFGF by Akt in endometrial cancer cells are mediated through the NF-κB pathway. Oncology Reports, 36, 705-714. https://doi.org/10.3892/or.2016.4888
MLA
Zhang, J., Song, H., Lu, Y., Chen, H., Jiang, S., Li, L."Effects of estradiol on VEGF and bFGF by Akt in endometrial cancer cells are mediated through the NF-κB pathway". Oncology Reports 36.2 (2016): 705-714.
Chicago
Zhang, J., Song, H., Lu, Y., Chen, H., Jiang, S., Li, L."Effects of estradiol on VEGF and bFGF by Akt in endometrial cancer cells are mediated through the NF-κB pathway". Oncology Reports 36, no. 2 (2016): 705-714. https://doi.org/10.3892/or.2016.4888
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Song H, Lu Y, Chen H, Jiang S and Li L: Effects of estradiol on VEGF and bFGF by Akt in endometrial cancer cells are mediated through the NF-κB pathway. Oncol Rep 36: 705-714, 2016.
APA
Zhang, J., Song, H., Lu, Y., Chen, H., Jiang, S., & Li, L. (2016). Effects of estradiol on VEGF and bFGF by Akt in endometrial cancer cells are mediated through the NF-κB pathway. Oncology Reports, 36, 705-714. https://doi.org/10.3892/or.2016.4888
MLA
Zhang, J., Song, H., Lu, Y., Chen, H., Jiang, S., Li, L."Effects of estradiol on VEGF and bFGF by Akt in endometrial cancer cells are mediated through the NF-κB pathway". Oncology Reports 36.2 (2016): 705-714.
Chicago
Zhang, J., Song, H., Lu, Y., Chen, H., Jiang, S., Li, L."Effects of estradiol on VEGF and bFGF by Akt in endometrial cancer cells are mediated through the NF-κB pathway". Oncology Reports 36, no. 2 (2016): 705-714. https://doi.org/10.3892/or.2016.4888
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