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Article

DT-13 inhibits cancer cell migration by regulating NMIIA indirectly in the tumor microenvironment

  • Authors:
    • Hongzhi Du
    • Yue Huang
    • Xiaoyin Hou
    • Xiaowen Yu
    • Sensen Lin
    • Xiaohui Wei
    • Ruiming Li
    • Ghulam Jilany Khan
    • Shengtao Yuan
    • Li Sun
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China, Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China, Tasly Research Institute, Tianjin Tasly Holding Group Co., Ltd., Tianjin 300410, P.R. China
  • Pages: 721-728
    |
    Published online on: June 22, 2016
       https://doi.org/10.3892/or.2016.4890
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Abstract

Tumor metastasis is one of the main causes of mortality among patients with malignant tumors. Previous studies concerning tumor metastasis have merely focused on the cancer cells in the tumor. However, an increasing number of studies show that the tumor microenvironment plays a vital role in the progression of cancer, particularly in tumor metastasis. Since fibroblasts and adipocytes are two of the most representative mesenchymal cells in the tumor microenvironment, we established a hypoxia-induced cancer-associated fibroblast (CAF) model and a chemically induced adipocyte model to reveal the effect of the microenvironment on cancer development. In these models, the conditioned medium from the tumor microenvironment was found to significantly promote the migration of human lung cancer cell line 95D and regulate the expression of non-muscle myosin IIA (NMIIA), which is consistent with results in the published literature. Then, we confirmed the hypothesis that the tumor microenvironment can regulate NMIIA in cancer cells and facilitate migration by using the non-muscle myosin II inhibitor, blebbistatin. Thus, this is the first report that the tumor microenvironment can promote cancer cell migration by regulating the expression of NMIIA. Our present data also indicated that DT-13, the saponin monomer 13 of dwarf lilyturf tuber, inhibited cancer cell migration in the tumor microenvironment model. Further results showed that DT-13 exhibited anti-migratory effects by inhibiting the c-raf/ERK1/2 signaling pathway. Consequently, our research confirmed that DT-13 significantly inhibited 95D cell migration in vitro, indicating the potential anti-metastatic effect of DT-13 on lung cancer and the scientific basis for drug development.
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Copy and paste a formatted citation
Spandidos Publications style
Du H, Huang Y, Hou X, Yu X, Lin S, Wei X, Li R, Khan GJ, Yuan S, Sun L, Sun L, et al: DT-13 inhibits cancer cell migration by regulating NMIIA indirectly in the tumor microenvironment. Oncol Rep 36: 721-728, 2016.
APA
Du, H., Huang, Y., Hou, X., Yu, X., Lin, S., Wei, X. ... Sun, L. (2016). DT-13 inhibits cancer cell migration by regulating NMIIA indirectly in the tumor microenvironment. Oncology Reports, 36, 721-728. https://doi.org/10.3892/or.2016.4890
MLA
Du, H., Huang, Y., Hou, X., Yu, X., Lin, S., Wei, X., Li, R., Khan, G. J., Yuan, S., Sun, L."DT-13 inhibits cancer cell migration by regulating NMIIA indirectly in the tumor microenvironment". Oncology Reports 36.2 (2016): 721-728.
Chicago
Du, H., Huang, Y., Hou, X., Yu, X., Lin, S., Wei, X., Li, R., Khan, G. J., Yuan, S., Sun, L."DT-13 inhibits cancer cell migration by regulating NMIIA indirectly in the tumor microenvironment". Oncology Reports 36, no. 2 (2016): 721-728. https://doi.org/10.3892/or.2016.4890
Copy and paste a formatted citation
x
Spandidos Publications style
Du H, Huang Y, Hou X, Yu X, Lin S, Wei X, Li R, Khan GJ, Yuan S, Sun L, Sun L, et al: DT-13 inhibits cancer cell migration by regulating NMIIA indirectly in the tumor microenvironment. Oncol Rep 36: 721-728, 2016.
APA
Du, H., Huang, Y., Hou, X., Yu, X., Lin, S., Wei, X. ... Sun, L. (2016). DT-13 inhibits cancer cell migration by regulating NMIIA indirectly in the tumor microenvironment. Oncology Reports, 36, 721-728. https://doi.org/10.3892/or.2016.4890
MLA
Du, H., Huang, Y., Hou, X., Yu, X., Lin, S., Wei, X., Li, R., Khan, G. J., Yuan, S., Sun, L."DT-13 inhibits cancer cell migration by regulating NMIIA indirectly in the tumor microenvironment". Oncology Reports 36.2 (2016): 721-728.
Chicago
Du, H., Huang, Y., Hou, X., Yu, X., Lin, S., Wei, X., Li, R., Khan, G. J., Yuan, S., Sun, L."DT-13 inhibits cancer cell migration by regulating NMIIA indirectly in the tumor microenvironment". Oncology Reports 36, no. 2 (2016): 721-728. https://doi.org/10.3892/or.2016.4890
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