IKK inhibitor suppresses epithelial-mesenchymal transition and induces cell death in prostate cancer

  • Authors:
    • Hao Ping
    • Feiya Yang
    • Mingshuai Wang
    • Yinong Niu
    • Nianzeng Xing
  • View Affiliations

  • Published online on: July 7, 2016     https://doi.org/10.3892/or.2016.4915
  • Pages: 1658-1664
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Abstract

IκB kinase (IKK)/nuclear factor κB (NF-κB) pathway activation is a key event in the acquisition of invasive and metastatic capacities in prostate cancer. A potent small-molecule compound, BMS-345541, was identified as a highly selective IKKα and IKKβ inhibitor to inhibit kinase activity. This study explored the effect of IKK inhibitor on epithelial-mesenchymal transition (EMT), apoptosis and metastasis in prostate cancer. Here, we demonstrate the role of IKK inhibitor reducing proliferation and inducing apoptosis in PC-3 cells. Furthermore, BMS345541 inhibited IκBα phosphorylation and nuclear level of NF-κB/p65 in PC-3 cells. We also observed downregulation of the N-cadherin, Snail, Slug and Twist protein in a dose-dependent manner. BMS‑345541 induced upregulation of the epithelial marker E-cadherin and phosphorylated NDRG1 at protein level. Moreover, BMS‑345541 reduced invasion and metastasis of PC-3 cells in vitro. In conclusion, IKK has a key role in both EMT and apoptosis of prostate cancer. IKK inhibitor can reverse EMT and induce cell death in PCa cells. IKK was identified as a potential target structure for future therapeutic intervention in PCa.
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September-2016
Volume 36 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ping H, Yang F, Wang M, Niu Y and Xing N: IKK inhibitor suppresses epithelial-mesenchymal transition and induces cell death in prostate cancer. Oncol Rep 36: 1658-1664, 2016
APA
Ping, H., Yang, F., Wang, M., Niu, Y., & Xing, N. (2016). IKK inhibitor suppresses epithelial-mesenchymal transition and induces cell death in prostate cancer. Oncology Reports, 36, 1658-1664. https://doi.org/10.3892/or.2016.4915
MLA
Ping, H., Yang, F., Wang, M., Niu, Y., Xing, N."IKK inhibitor suppresses epithelial-mesenchymal transition and induces cell death in prostate cancer". Oncology Reports 36.3 (2016): 1658-1664.
Chicago
Ping, H., Yang, F., Wang, M., Niu, Y., Xing, N."IKK inhibitor suppresses epithelial-mesenchymal transition and induces cell death in prostate cancer". Oncology Reports 36, no. 3 (2016): 1658-1664. https://doi.org/10.3892/or.2016.4915