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Article

Genetically engineered Newcastle disease virus expressing human interferon-λ1 induces apoptosis in gastric adenocarcinoma cells and modulates the Th1/Th2 immune response

  • Authors:
    • Xuefeng Bu
    • Mi Li
    • Yinghai Zhao
    • Sha Liu
    • Mubin Wang
    • Jinying Ge
    • Zhigao Bu
    • Yulan Yan
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China, Clinical Medicine College of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150001, P.R. China, Department of Internal Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China
  • Pages: 1393-1402
    |
    Published online on: July 11, 2016
       https://doi.org/10.3892/or.2016.4925
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Abstract

Interferon-λ1 (IFN-λ1), a recently discovered cytokine of the type III IFN family, was found to be a therapeutic alternative to type I IFN in terms of tumors. Using reverse genetics technique, we generated a recombinant Newcastle disease virus (NDV) LaSota strains named as human IFN‑λ1 recombinant adenovirus (rL-hIFN-λ1) containing human IFN-λ1 gene and further evaluated the expressing of IFN-λ1 in human gastric adenocarcinoma cell line SGC-7901 after infected with rL-hIFN-λ1 by using western blot analysis, RT-PCR and immunofluorescence analyses. IFN-λl specific receptor IFNLR1 was detected on several gastric tumor cell lines including SGC-7901 and AGS and on PBMCs.The expression of the IFN-λ1 proteins reached a high level detected in the supernatant harvested 24 h after the infection of tumor cells. The proliferation changes of SGC infected with rL-hIFN-λ1 was significantly inhibited compared with NDV-infected group. Apoptosis was significantly induced by rL-hIFN-λ1 in gastric cancer cells compared with NDV virus tested by TUNEL assay, western blot analysis and Annexin V flow cytometry. Due to the high dose of IFN-λ1 expressed by the rL-hIFN-λ1-infected tumor cells, the immune study showed that rL-hIFN-λ1 increased IFN-γ production [the T helper cell subtype 1 (Th1) response] and inhibited interleukin (IL)-13 production [the T helper cell subtype 2 (Th2) response] to change the Th1/Th2 response of tumor microenvironment which inhibited tumor growth. This study aims at building recombinant NDV rL-hIFN-λ1 as an efficient antitumor agent.
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Copy and paste a formatted citation
Spandidos Publications style
Bu X, Li M, Zhao Y, Liu S, Wang M, Ge J, Bu Z and Yan Y: Genetically engineered Newcastle disease virus expressing human interferon-λ1 induces apoptosis in gastric adenocarcinoma cells and modulates the Th1/Th2 immune response. Oncol Rep 36: 1393-1402, 2016.
APA
Bu, X., Li, M., Zhao, Y., Liu, S., Wang, M., Ge, J. ... Yan, Y. (2016). Genetically engineered Newcastle disease virus expressing human interferon-λ1 induces apoptosis in gastric adenocarcinoma cells and modulates the Th1/Th2 immune response. Oncology Reports, 36, 1393-1402. https://doi.org/10.3892/or.2016.4925
MLA
Bu, X., Li, M., Zhao, Y., Liu, S., Wang, M., Ge, J., Bu, Z., Yan, Y."Genetically engineered Newcastle disease virus expressing human interferon-λ1 induces apoptosis in gastric adenocarcinoma cells and modulates the Th1/Th2 immune response". Oncology Reports 36.3 (2016): 1393-1402.
Chicago
Bu, X., Li, M., Zhao, Y., Liu, S., Wang, M., Ge, J., Bu, Z., Yan, Y."Genetically engineered Newcastle disease virus expressing human interferon-λ1 induces apoptosis in gastric adenocarcinoma cells and modulates the Th1/Th2 immune response". Oncology Reports 36, no. 3 (2016): 1393-1402. https://doi.org/10.3892/or.2016.4925
Copy and paste a formatted citation
x
Spandidos Publications style
Bu X, Li M, Zhao Y, Liu S, Wang M, Ge J, Bu Z and Yan Y: Genetically engineered Newcastle disease virus expressing human interferon-λ1 induces apoptosis in gastric adenocarcinoma cells and modulates the Th1/Th2 immune response. Oncol Rep 36: 1393-1402, 2016.
APA
Bu, X., Li, M., Zhao, Y., Liu, S., Wang, M., Ge, J. ... Yan, Y. (2016). Genetically engineered Newcastle disease virus expressing human interferon-λ1 induces apoptosis in gastric adenocarcinoma cells and modulates the Th1/Th2 immune response. Oncology Reports, 36, 1393-1402. https://doi.org/10.3892/or.2016.4925
MLA
Bu, X., Li, M., Zhao, Y., Liu, S., Wang, M., Ge, J., Bu, Z., Yan, Y."Genetically engineered Newcastle disease virus expressing human interferon-λ1 induces apoptosis in gastric adenocarcinoma cells and modulates the Th1/Th2 immune response". Oncology Reports 36.3 (2016): 1393-1402.
Chicago
Bu, X., Li, M., Zhao, Y., Liu, S., Wang, M., Ge, J., Bu, Z., Yan, Y."Genetically engineered Newcastle disease virus expressing human interferon-λ1 induces apoptosis in gastric adenocarcinoma cells and modulates the Th1/Th2 immune response". Oncology Reports 36, no. 3 (2016): 1393-1402. https://doi.org/10.3892/or.2016.4925
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