Classical and atypical resistance of cancer cells as a target for resveratrol

  • Authors:
    • Sylwia Borska
    • Monika Pedziwiatr
    • Monika Danielewicz
    • Katarzyna Nowinska
    • Bartosz Pula
    • Malgorzata Drag-Zalesinska
    • Mateusz Olbromski
    • Agnieszka Gomulkiewicz
    • Piotr Dziegiel
  • View Affiliations

  • Published online on: July 11, 2016     https://doi.org/10.3892/or.2016.4930
  • Pages: 1562-1568
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Abstract

The phenomenon of cancer cell resistance to chemotherapeutics is the main cause of insensitivity to anticancer therapy. Thus, the current challenge remains searching for substances sensitising the activity of cytostatic drugs. In this respect, resveratrol is a very promising therapeutic agent. It has pleiotropic effect on cancer cells, which can play a key role in numerous resistance mechanisms, both classical and atypical. The purpose of the present study was to assess the effect of resveratrol on the inhibition of human pancreatic cancer cell proliferation and on the level of cytostatic resistance-associated proteins. The study was performed on human pancreatic cancer cell lines EPP85-181P (control), EPP85-181RDB (daunorubicin resistance) and EPP85-181PRNOV (mitoxantrone resistance). The effect of resveratrol on the viability and proliferation of the studied cell lines was evaluated by SRB assay, whereas cell cycle arrest and cytostatic accumulation by FACS. Western blot analysis was used to determine the level of P-glycoprotein, topoisomerase II α and β and immunofluorescence technique to visualise the proteins in the cells. Resveratrol inhibited proliferation of all studied cell lines. Phase-specific cell cycle arrest depended on the type of cancer cells. Resveratrol decreased the level and activity of P-gp in EPP85-181RDB cells. In EPP85-181PRNOV cells, expression of both TopoII isoforms increased in a statistically significant manner. The results of in vitro studies support the possibility of potential use of resveratrol in breaking cancer cell resistance to chemotherapeutic drugs.
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September-2016
Volume 36 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Borska S, Pedziwiatr M, Danielewicz M, Nowinska K, Pula B, Drag-Zalesinska M, Olbromski M, Gomulkiewicz A and Dziegiel P: Classical and atypical resistance of cancer cells as a target for resveratrol. Oncol Rep 36: 1562-1568, 2016
APA
Borska, S., Pedziwiatr, M., Danielewicz, M., Nowinska, K., Pula, B., Drag-Zalesinska, M. ... Dziegiel, P. (2016). Classical and atypical resistance of cancer cells as a target for resveratrol. Oncology Reports, 36, 1562-1568. https://doi.org/10.3892/or.2016.4930
MLA
Borska, S., Pedziwiatr, M., Danielewicz, M., Nowinska, K., Pula, B., Drag-Zalesinska, M., Olbromski, M., Gomulkiewicz, A., Dziegiel, P."Classical and atypical resistance of cancer cells as a target for resveratrol". Oncology Reports 36.3 (2016): 1562-1568.
Chicago
Borska, S., Pedziwiatr, M., Danielewicz, M., Nowinska, K., Pula, B., Drag-Zalesinska, M., Olbromski, M., Gomulkiewicz, A., Dziegiel, P."Classical and atypical resistance of cancer cells as a target for resveratrol". Oncology Reports 36, no. 3 (2016): 1562-1568. https://doi.org/10.3892/or.2016.4930