RNA interference against TRIM29 inhibits migration and invasion of colorectal cancer cells

  • Authors:
    • Weihong Xu
    • Bin Xu
    • Yiting Yao
    • Xiaoling Yu
    • Hua Cao
    • Jun Zhang
    • Jie Liu
    • Huiming Sheng
  • View Affiliations

  • Published online on: July 15, 2016     https://doi.org/10.3892/or.2016.4941
  • Pages: 1411-1418
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Abstract

Colorectal cancer (CRC) is one of the most common malignancies worldwide. Tripartite motif-containing 29 (TRIM29) is a member of TRIM proteins family, which plays diverse physiological and pathological roles in humans. Recent studies found that TRIM29 expressed highly in CRC and promoted cell growth in vitro. However, its function in the metastasis of CRC has not been studied. In the present study, we confirmed the previous report that TRIM29 was upregulated in CRC tissues and high levels of TRIM29 expression were associated with poor overall survival of patients. Moreover, TRIM29 knockdown significantly reduced cancer cell proliferation via notably inducing cell cycle arrest and cell apoptosis. Silencing of TRIM29 significantly inhibited the migration and invasion ability of CRC cells. The protein levels of apoptosis‑, migration‑ and invasion‑related proteins were also changed after TRIM29 knockdown. Furthermore, phosphorylation levels of JAK2 and STAT3 were clearly reduced in TRIM29 knockdown cells, indicating a possible mechanism underlying its effects on colorectal carcinogenesis. Collectively, TRIM29 may exert oncogenic effects in CRC cells via regulating JAK2/STAT3 signaling.
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September-2016
Volume 36 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Xu W, Xu B, Yao Y, Yu X, Cao H, Zhang J, Liu J and Sheng H: RNA interference against TRIM29 inhibits migration and invasion of colorectal cancer cells. Oncol Rep 36: 1411-1418, 2016
APA
Xu, W., Xu, B., Yao, Y., Yu, X., Cao, H., Zhang, J. ... Sheng, H. (2016). RNA interference against TRIM29 inhibits migration and invasion of colorectal cancer cells. Oncology Reports, 36, 1411-1418. https://doi.org/10.3892/or.2016.4941
MLA
Xu, W., Xu, B., Yao, Y., Yu, X., Cao, H., Zhang, J., Liu, J., Sheng, H."RNA interference against TRIM29 inhibits migration and invasion of colorectal cancer cells". Oncology Reports 36.3 (2016): 1411-1418.
Chicago
Xu, W., Xu, B., Yao, Y., Yu, X., Cao, H., Zhang, J., Liu, J., Sheng, H."RNA interference against TRIM29 inhibits migration and invasion of colorectal cancer cells". Oncology Reports 36, no. 3 (2016): 1411-1418. https://doi.org/10.3892/or.2016.4941