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Article

Sulforaphane-cysteine suppresses invasion via downregulation of galectin-1 in human prostate cancer DU145 and PC3 cells

  • Authors:
    • Hua Tian
    • Yan Zhou
    • Gaoxiang Yang
    • Yang Geng
    • Sai Wu
    • Yabin Hu
    • Kai Lin
    • Wei Wu
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, P.R. China
  • Pages: 1361-1368
    |
    Published online on: July 15, 2016
       https://doi.org/10.3892/or.2016.4942
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Abstract

Our previous study showed that sulforaphane (SFN) inhibits invasion in human prostate cancer DU145 cells; however, the underlying mechanisms were not profoundly investigated. In the present study, we found that sulforaphane-cysteine (SFN-Cys), as a metabolite of SFN, inhibits invasion and possesses a novel mechanism in prostate cancer DU145 and PC3 cells. The scratch and Transwell assays showed that SFN-Cys (15 µM) inhibited both migration and invasion, with cell morphological changes, such as cell shrinkage and pseudopodia shortening. The cell proliferation (MTS) assay indicated that cell viability was markedly suppressed with increasing concentrations of SFN‑Cys. Furthermore, the Transwell assay showed that inhibition of SFN‑Cys‑triggered invasion was tightly linked to the sustained extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation. Western blot analysis revealed that SFN-Cys downregulated galectin-1 protein, an invasion‑related protein, and that the galectin‑1 reduction could be blocked by ERK1/2 inhibitor PD98059 (25 µM). Moreover, immunofluorescence staining showed that the expression level of galectin-1 protein was significantly reduced in the cells treated with SFN‑Cys. Hence, SFN‑Cys‑inhibited invasion resulted from the sustained ERK1/2 phosphorylation and ERK1/2‑triggered galectin-1 downregulation, suggesting that galectin-1 is a new SFN-Cys target inhibiting invasion apart from ERK1/2, in the treatment of prostate cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Tian H, Zhou Y, Yang G, Geng Y, Wu S, Hu Y, Lin K and Wu W: Sulforaphane-cysteine suppresses invasion via downregulation of galectin-1 in human prostate cancer DU145 and PC3 cells. Oncol Rep 36: 1361-1368, 2016.
APA
Tian, H., Zhou, Y., Yang, G., Geng, Y., Wu, S., Hu, Y. ... Wu, W. (2016). Sulforaphane-cysteine suppresses invasion via downregulation of galectin-1 in human prostate cancer DU145 and PC3 cells. Oncology Reports, 36, 1361-1368. https://doi.org/10.3892/or.2016.4942
MLA
Tian, H., Zhou, Y., Yang, G., Geng, Y., Wu, S., Hu, Y., Lin, K., Wu, W."Sulforaphane-cysteine suppresses invasion via downregulation of galectin-1 in human prostate cancer DU145 and PC3 cells". Oncology Reports 36.3 (2016): 1361-1368.
Chicago
Tian, H., Zhou, Y., Yang, G., Geng, Y., Wu, S., Hu, Y., Lin, K., Wu, W."Sulforaphane-cysteine suppresses invasion via downregulation of galectin-1 in human prostate cancer DU145 and PC3 cells". Oncology Reports 36, no. 3 (2016): 1361-1368. https://doi.org/10.3892/or.2016.4942
Copy and paste a formatted citation
x
Spandidos Publications style
Tian H, Zhou Y, Yang G, Geng Y, Wu S, Hu Y, Lin K and Wu W: Sulforaphane-cysteine suppresses invasion via downregulation of galectin-1 in human prostate cancer DU145 and PC3 cells. Oncol Rep 36: 1361-1368, 2016.
APA
Tian, H., Zhou, Y., Yang, G., Geng, Y., Wu, S., Hu, Y. ... Wu, W. (2016). Sulforaphane-cysteine suppresses invasion via downregulation of galectin-1 in human prostate cancer DU145 and PC3 cells. Oncology Reports, 36, 1361-1368. https://doi.org/10.3892/or.2016.4942
MLA
Tian, H., Zhou, Y., Yang, G., Geng, Y., Wu, S., Hu, Y., Lin, K., Wu, W."Sulforaphane-cysteine suppresses invasion via downregulation of galectin-1 in human prostate cancer DU145 and PC3 cells". Oncology Reports 36.3 (2016): 1361-1368.
Chicago
Tian, H., Zhou, Y., Yang, G., Geng, Y., Wu, S., Hu, Y., Lin, K., Wu, W."Sulforaphane-cysteine suppresses invasion via downregulation of galectin-1 in human prostate cancer DU145 and PC3 cells". Oncology Reports 36, no. 3 (2016): 1361-1368. https://doi.org/10.3892/or.2016.4942
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