STC-1 expression is upregulated through an Akt/NF-κB-dependent pathway in triple-negative breast cancer cells

  • Authors:
    • Myeongjin Jeon
    • Jeonghun Han
    • Seok Jin Nam
    • Jeong Eon Lee
    • Sangmin Kim
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  • Published online on: July 25, 2016     https://doi.org/10.3892/or.2016.4972
  • Pages: 1717-1722
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Abstract

Stanniocalcin-1 (STC-1) is a secreted glycoprotein and its expression is strongly correlated with cancer development. However, regulatory mechanism of STC-1 expression in breast cancer cells is not clear. In the present study, we investigated whether STC-1 acts as a prognostic factor in TNBC patients and the regulation of STC-1 expression in breast cancer cells. Basal levels of STC-1 were significantly higher in TNBC cells than in non-TNBC cells. Induction of STC-1 expression was also associated with poor relapse-free survival of TNBC patients. In addition, we verified the correlation between the invasiveness of TNBC cells and the STC-1 expression. We found that recombinant human STC-1 treatment increased the invasiveness of TNBC cells. In contrast, STC-1-induced cell invasiveness was completely inhibited by anti-STC-1 monoclonal antibody treatment. We found that the basal levels of STC-1 expression in TNBC cells were decreased by treatment with LY294002 or Bay11-7085, but not SB203580. In contrast, transcript levels of STC-1 and protein secretion were increased by constitutively active Akt (CA-Akt) or NF-κB overexpression in TNBC cells. Finally, we observed that phosphorylation of NF-κB was significantly increased by CA-Akt overexpression in TNBC cells. Taken together, elevated STC-1 expression is associated with poor clinical outcome in TNBC patients, and STC-1 is directly involved in the invasiveness of TNBC cells. STC-1 expression is upregulated through a PI-3K/Akt/NF-κB‑dependent signaling pathway in TNBC cells.
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September-2016
Volume 36 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Jeon M, Han J, Nam SJ, Lee JE and Kim S: STC-1 expression is upregulated through an Akt/NF-κB-dependent pathway in triple-negative breast cancer cells. Oncol Rep 36: 1717-1722, 2016
APA
Jeon, M., Han, J., Nam, S.J., Lee, J.E., & Kim, S. (2016). STC-1 expression is upregulated through an Akt/NF-κB-dependent pathway in triple-negative breast cancer cells. Oncology Reports, 36, 1717-1722. https://doi.org/10.3892/or.2016.4972
MLA
Jeon, M., Han, J., Nam, S. J., Lee, J. E., Kim, S."STC-1 expression is upregulated through an Akt/NF-κB-dependent pathway in triple-negative breast cancer cells". Oncology Reports 36.3 (2016): 1717-1722.
Chicago
Jeon, M., Han, J., Nam, S. J., Lee, J. E., Kim, S."STC-1 expression is upregulated through an Akt/NF-κB-dependent pathway in triple-negative breast cancer cells". Oncology Reports 36, no. 3 (2016): 1717-1722. https://doi.org/10.3892/or.2016.4972