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Article

MicroRNA-520a attenuates proliferation of Raji cells through inhibition of AKT1/NF-κB and PERK/eIF2α signaling pathway

Retraction in: /10.3892/or.2024.8706
  • Authors:
    • Xiaojuan Wang
    • Pei Wang
    • Yan Zhu
    • Zhi Zhang
    • Jinqian Zhang
    • Hongwei Wang
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Laboratory Medicine, The Second People's Hospital of Guangdong Province, Southern Medical University, Guangzhou, Guangdong 510317, P.R. China
  • Pages: 1702-1708
    |
    Published online on: July 25, 2016
       https://doi.org/10.3892/or.2016.4975
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Abstract

Burkitt's lymphoma (BL) is a fast growing cancer of the human lymphatic system, and an extremely invasive B-cell non-Hodgkin's lymphoma. We explored the mechanism of apoptosis in Raji cells associated with the post-transcriptional regulation factors. To confirm that the predicted microRNA-520a (miR-520a) is matched with AKT1, 3' untranslated region (UTR) luciferase activity of AKT1 was used in the assessment. In the presence of the mimics or inhibitors of miR-520a, cell function of Raji, such as proliferation, growth and apoptosis were analyzed. The expression of endoplasmic reticulum (ER) stress‑related proteins were examined. Luciferase reporter analysis showed that miR‑520a leads to decreased activity of luciferase gene fused with AKT1 3'UTR. Therefore, AKT1 is a direct target of miR‑520a. Our data indicated that the mimics of miR‑520a inhibited growth, proliferation of Raji cells and promoted its apoptosis, which was related to downregulation of AKT1, NF‑κB and ER stress response mediated by PERK/eIF2α pathway. On the contrary, the inhibitors of miR‑520a promoted growth, proliferation of Raji cells and inhibited its apoptosis, which was related to AKT1/NF‑κB and PERK/eIF2α pathway. We identified miR‑520a, which specifically binds to AKT1 mRNA 3'UTR. miR‑520a is a crucial mediator for proliferation and ER stress in Raji cells through regulating the AKT1/NF‑κB or PERK/eIF2α signaling pathway. Our findings suggest that targeting miR‑520a is a promising therapeutic strategy in BL.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Wang P, Zhu Y, Zhang Z, Zhang J and Wang H: MicroRNA-520a attenuates proliferation of Raji cells through inhibition of AKT1/NF-κB and PERK/eIF2α signaling pathway Retraction in /10.3892/or.2024.8706. Oncol Rep 36: 1702-1708, 2016.
APA
Wang, X., Wang, P., Zhu, Y., Zhang, Z., Zhang, J., & Wang, H. (2016). MicroRNA-520a attenuates proliferation of Raji cells through inhibition of AKT1/NF-κB and PERK/eIF2α signaling pathway Retraction in /10.3892/or.2024.8706. Oncology Reports, 36, 1702-1708. https://doi.org/10.3892/or.2016.4975
MLA
Wang, X., Wang, P., Zhu, Y., Zhang, Z., Zhang, J., Wang, H."MicroRNA-520a attenuates proliferation of Raji cells through inhibition of AKT1/NF-κB and PERK/eIF2α signaling pathway Retraction in /10.3892/or.2024.8706". Oncology Reports 36.3 (2016): 1702-1708.
Chicago
Wang, X., Wang, P., Zhu, Y., Zhang, Z., Zhang, J., Wang, H."MicroRNA-520a attenuates proliferation of Raji cells through inhibition of AKT1/NF-κB and PERK/eIF2α signaling pathway Retraction in /10.3892/or.2024.8706". Oncology Reports 36, no. 3 (2016): 1702-1708. https://doi.org/10.3892/or.2016.4975
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Wang P, Zhu Y, Zhang Z, Zhang J and Wang H: MicroRNA-520a attenuates proliferation of Raji cells through inhibition of AKT1/NF-κB and PERK/eIF2α signaling pathway Retraction in /10.3892/or.2024.8706. Oncol Rep 36: 1702-1708, 2016.
APA
Wang, X., Wang, P., Zhu, Y., Zhang, Z., Zhang, J., & Wang, H. (2016). MicroRNA-520a attenuates proliferation of Raji cells through inhibition of AKT1/NF-κB and PERK/eIF2α signaling pathway Retraction in /10.3892/or.2024.8706. Oncology Reports, 36, 1702-1708. https://doi.org/10.3892/or.2016.4975
MLA
Wang, X., Wang, P., Zhu, Y., Zhang, Z., Zhang, J., Wang, H."MicroRNA-520a attenuates proliferation of Raji cells through inhibition of AKT1/NF-κB and PERK/eIF2α signaling pathway Retraction in /10.3892/or.2024.8706". Oncology Reports 36.3 (2016): 1702-1708.
Chicago
Wang, X., Wang, P., Zhu, Y., Zhang, Z., Zhang, J., Wang, H."MicroRNA-520a attenuates proliferation of Raji cells through inhibition of AKT1/NF-κB and PERK/eIF2α signaling pathway Retraction in /10.3892/or.2024.8706". Oncology Reports 36, no. 3 (2016): 1702-1708. https://doi.org/10.3892/or.2016.4975
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