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Article

Fucoidan inhibits angiogenesis induced by multiple myeloma cells

  • Authors:
    • Fen Liu
    • Guoping Luo
    • Qing Xiao
    • Liping Chen
    • Xiaohua Luo
    • Jinglong Lv
    • Lixue Chen
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, The Central Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 1963-1972
    |
    Published online on: August 1, 2016
       https://doi.org/10.3892/or.2016.4987
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Abstract

Multiple myeloma (MM) remains an incurable hematological neoplasms. Our previous studies showed that Fucoidan possessed anti-myeloma effect by inducing apoptosis and inhibiting invasion of myeloma cells. In this study, we evaluated the effect of Fucoidan on angiogenesis induced by human myeloma cells and elucidated its possible mechanisms. Multiple myeloma cells were treated with Fucoidan at different concentrations, then the conditioned medium (CM) was collected. The levels of VEGF in the CM were tested by ELISA. The results showed that Fucoidan significantly decreased VEGF secretion by RPMI-8226 and U266 cells. The tube formation assay and migration test on human umbilical vein endothelial cells (HUVECs) were used to examine the effect of Fucoidan on angiogenesis induced by human myeloma cells. The results showed that Fucoidan decreased HUVECs formed tube structures and inhibited HUVECs migration, and suppressed the angiogenic ability of multiple myeloma RPMI-8226 and U266 cells in a dose-dependent manner. The study also showed that Fucoidan downregulated the expression of several kinds of proteins, which may be correlated with the reduction of angiogenesis induced by myeloma cells. Moreover, results were compared from normoxic and hypoxic conditions, they showed that Fucoidan had anti-angiogenic activity. Furthermore, in a multiple myeloma xenograft mouse model, it indicated that Fucoidan negatively affected tumor growth and angiogenesis in vivo. In conclusion, our results demonstrate that Fucoidan was able to interfere with angiogenesis of multiple myeloma cells both in vitro and in vivo and may have a substantial potential in the treatment of MM.
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Copy and paste a formatted citation
Spandidos Publications style
Liu F, Luo G, Xiao Q, Chen L, Luo X, Lv J and Chen L: Fucoidan inhibits angiogenesis induced by multiple myeloma cells. Oncol Rep 36: 1963-1972, 2016.
APA
Liu, F., Luo, G., Xiao, Q., Chen, L., Luo, X., Lv, J., & Chen, L. (2016). Fucoidan inhibits angiogenesis induced by multiple myeloma cells. Oncology Reports, 36, 1963-1972. https://doi.org/10.3892/or.2016.4987
MLA
Liu, F., Luo, G., Xiao, Q., Chen, L., Luo, X., Lv, J., Chen, L."Fucoidan inhibits angiogenesis induced by multiple myeloma cells". Oncology Reports 36.4 (2016): 1963-1972.
Chicago
Liu, F., Luo, G., Xiao, Q., Chen, L., Luo, X., Lv, J., Chen, L."Fucoidan inhibits angiogenesis induced by multiple myeloma cells". Oncology Reports 36, no. 4 (2016): 1963-1972. https://doi.org/10.3892/or.2016.4987
Copy and paste a formatted citation
x
Spandidos Publications style
Liu F, Luo G, Xiao Q, Chen L, Luo X, Lv J and Chen L: Fucoidan inhibits angiogenesis induced by multiple myeloma cells. Oncol Rep 36: 1963-1972, 2016.
APA
Liu, F., Luo, G., Xiao, Q., Chen, L., Luo, X., Lv, J., & Chen, L. (2016). Fucoidan inhibits angiogenesis induced by multiple myeloma cells. Oncology Reports, 36, 1963-1972. https://doi.org/10.3892/or.2016.4987
MLA
Liu, F., Luo, G., Xiao, Q., Chen, L., Luo, X., Lv, J., Chen, L."Fucoidan inhibits angiogenesis induced by multiple myeloma cells". Oncology Reports 36.4 (2016): 1963-1972.
Chicago
Liu, F., Luo, G., Xiao, Q., Chen, L., Luo, X., Lv, J., Chen, L."Fucoidan inhibits angiogenesis induced by multiple myeloma cells". Oncology Reports 36, no. 4 (2016): 1963-1972. https://doi.org/10.3892/or.2016.4987
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