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Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells

  • Authors:
    • Xiaohui Yang
    • Shuai Wang
    • Yunchuan Mu
    • Yixiong Zheng
  • View Affiliations / Copyright

    Affiliations: The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, P.R. China, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1799-1806
    |
    Published online on: August 2, 2016
       https://doi.org/10.3892/or.2016.4992
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Abstract

Cholangiocarcinoma (CCA) is the second most common hepatic cancer with high resistance to current chemotherapies and extremely poor prognosis. The present study aimed to examine the effects of schisandrin B (Sch B) on CCA cells both in vitro and in vivo and to examine its underlying mechanism. We found that Sch B inhibited the viability and proliferation of CCA cells in a dose- and time-dependent manner as assessed by MTT and colony formation assays. The flow cytometric assay revealed G0/G1 phase arrest in the Sch B-treated HCCC-9810 and RBE cells. In addition, Sch B induced intrahepatic cholangiocarcinoma apoptosis as shown by the results of Annexin V/PI double staining. Rhodamine 123 staining revealed that Sch B decreased the mitochondrial membrane potential (ΔΨm) in a dose-dependent manner. Mechanistically, western blot analysis indicated that Sch B induced apoptosis by upregulating Bax, cleaved caspase-3, cleaved caspase-9 and cleaved PARP, and by downregulating cyclin D1, Bcl-2 and CDK-4. Moreover, Sch B significantly inhibited HCCC-9810 xenograft growth in athymic nude mice. In summary, these findings suggest that Sch B exhibited potent antitumor activities via the induction of CCA apoptosis and that Sch B may be a promising drug for the treatment of CCA.
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1 

de Groen PC, Gores GJ, LaRusso NF, Gunderson LL and Nagorney DM: Biliary tract cancers. N Engl J Med. 341:1368–1378. 1999. View Article : Google Scholar : PubMed/NCBI

2 

Tyson GL, Ilyas JA, Duan Z, Green LK, Younes M, El-Serag HB and Davila JA: Secular trends in the incidence of cholangiocarcinoma in the USA and the impact of misclassification. Dig Dis Sci. 59:3103–3110. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Landis SH, Murray T, Bolden S and Wingo PA: Cancer statistics, 1998. CA Cancer J Clin. 48:6–29. 1998. View Article : Google Scholar : PubMed/NCBI

4 

Patel T: Worldwide trends in mortality from biliary tract malignancies. BMC Cancer. 2:102002. View Article : Google Scholar : PubMed/NCBI

5 

Khan SA, Davidson BR, Goldin R, Pereira SP, Rosenberg WM, Taylor-Robinson SD, Thillainayagam AV, Thomas HC, Thursz MR and Wasan H: British Society of Gastroenterology: Guidelines for the diagnosis and treatment of cholangiocarcinoma: Consensus document. Gut. 51(Suppl 6): VI1–VI9. 2002. View Article : Google Scholar

6 

Zhang TC, Cao EH, Li JF, Ma W and Qin JF: Induction of apoptosis and inhibition of human gastric cancer MGC-803 cell growth by arsenic trioxide. Eur J Cancer. 35:1258–1263. 1999. View Article : Google Scholar

7 

Shaib Y and El-Serag HB: The epidemiology of cholangiocarcinoma. Semin Liver Dis. 24:115–125. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Liu GT: Pharmacological actions and clinical use of fructus schizandrae. Chin Med J. 102:740–749. 1989.PubMed/NCBI

9 

Li L, Wang T, Xu ZL, Yu Y, Chen W and Chen F: Effects of schisandrin B on reversing multidrug resistance in human breast cancer cells transfected with mdr1 gene. Zhonghua Yi Xue Za Zhi. 85:1633–1637. 2005.In Chinese. PubMed/NCBI

10 

Liu XN, Zhang CY, Jin XD, Li YZ, Zheng XZ and Li L: Inhibitory effect of schisandrin B on gastric cancer cells in vitro. World J Gastroenterol. 13:6506–6511. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Wu YF, Cao MF, Gao YP, Chen F, Wang T, Zumbika EP and Qian KX: Down-modulation of heat shock protein 70 and up-modulation of caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells. World J Gastroenterol. 10:2944–2948. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Li Q, Lu XH, Wang CD, Cai L, Lu JL, Wu JS, Zhuge QC, Zheng WM and Su ZP: Antiproliferative and apoptosis-inducing activity of schisandrin B against human glioma cells. Cancer Cell Int. 15:122015. View Article : Google Scholar : PubMed/NCBI

13 

Liu Z, Zhang B, Liu K, Ding Z and Hu X: Schisandrin B attenuates cancer invasion and metastasis via inhibiting epithelial-mesenchymal transition. PLoS One. 7:e404802012. View Article : Google Scholar : PubMed/NCBI

14 

Nishida H, Tatewaki N, Nakajima Y, Magara T, Ko KM, Hamamori Y and Konishi T: Inhibition of ATR protein kinase activity by schisandrin B in DNA damage response. Nucleic Acids Res. 37:5678–5689. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Hu YP, Tan ZJ, Wu XS, Liu TY, Jiang L, Bao RF, Shu YJ, Li ML, Weng H, Ding Q, et al: Triptolide induces s phase arrest and apoptosis in gallbladder cancer cells. Molecules. 19:2612–2628. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Bloom J and Cross FR: Multiple levels of cyclin specificity in cell-cycle control. Nat Rev Mol Cell Biol. 8:149–160. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Nigg EA: Cyclin-dependent protein kinases: Key regulators of the eukaryotic cell cycle. BioEssays. 17:471–480. 1995. View Article : Google Scholar : PubMed/NCBI

18 

Harbour JW, Luo RX, Dei Santi A, Postigo AA and Dean DC: Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. Cell. 98:859–869. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Ezhevsky SA, Ho A, Becker-Hapak M, Davis PK and Dowdy SF: Differential regulation of retinoblastoma tumor suppressor protein by G1 cyclin-dependent kinase complexes in vivo. Mol Cell Biol. 21:4773–4784. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Hinds PW, Mittnacht S, Dulic V, Arnold A, Reed SI and Weinberg RA: Regulation of retinoblastoma protein functions by ectopic expression of human cyclins. Cell. 70:993–1006. 1992. View Article : Google Scholar : PubMed/NCBI

21 

Massagué J: G1 cell-cycle control and cancer. Nature. 432:298–306. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Chinkwo KA: Sutherlandia frutescens extracts can induce apoptosis in cultured carcinoma cells. J Ethnopharmacol. 98:163–170. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Asgar MA, Senawong G, Sripa B and Senawong T: Synergistic anticancer effects of cisplatin and histone deacetylase inhibitors (SAHA and TSA) on cholangiocarcinoma cell lines. Int J Oncol. 48:409–420. 2016.

24 

Hu W and Kavanagh JJ: Anticancer therapy targeting the apoptotic pathway. Lancet Oncol. 4:721–729. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Reed JC: Apoptosis-regulating proteins as targets for drug discovery. Trends Mol Med. 7:314–319. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Zheng L, Zheng J, Wu LJ and Zhao YY: Julibroside J8-induced HeLa cell apoptosis through caspase pathway. J Asian Nat Prod Res. 8:457–465. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Antonsson B: Mitochondria and the Bcl-2 family proteins in apoptosis signaling pathways. Mol Cell Biochem. 256–257:141–155. 2004. View Article : Google Scholar

28 

Pal S, Pal PB, Das J and Sil PC: Involvement of both intrinsic and extrinsic pathways in hepatoprotection of arjunolic acid against cadmium induced acute damage in vitro. Toxicology. 283:129–139. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Tsujimoto Y: Bcl-2 family of proteins: Life-or-death switch in mitochondria. Biosci Rep. 22:47–58. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Das A, Banik NL, Patel SJ and Ray SK: Dexamethasone protected human glioblastoma U87MG cells from temozolomide induced apoptosis by maintaining Bax:Bcl-2 ratio and preventing proteolytic activities. Mol Cancer. 3:362004. View Article : Google Scholar : PubMed/NCBI

31 

Zhang YH, Wu YL, Tashiro S, Onodera S and Ikejima T: Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells. Acta Pharmacol Sin. 32:1266–1275. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Wang S, Mu Y and Zheng Y: Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells. Oncol Rep 36: 1799-1806, 2016.
APA
Yang, X., Wang, S., Mu, Y., & Zheng, Y. (2016). Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells. Oncology Reports, 36, 1799-1806. https://doi.org/10.3892/or.2016.4992
MLA
Yang, X., Wang, S., Mu, Y., Zheng, Y."Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells". Oncology Reports 36.4 (2016): 1799-1806.
Chicago
Yang, X., Wang, S., Mu, Y., Zheng, Y."Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells". Oncology Reports 36, no. 4 (2016): 1799-1806. https://doi.org/10.3892/or.2016.4992
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Wang S, Mu Y and Zheng Y: Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells. Oncol Rep 36: 1799-1806, 2016.
APA
Yang, X., Wang, S., Mu, Y., & Zheng, Y. (2016). Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells. Oncology Reports, 36, 1799-1806. https://doi.org/10.3892/or.2016.4992
MLA
Yang, X., Wang, S., Mu, Y., Zheng, Y."Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells". Oncology Reports 36.4 (2016): 1799-1806.
Chicago
Yang, X., Wang, S., Mu, Y., Zheng, Y."Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells". Oncology Reports 36, no. 4 (2016): 1799-1806. https://doi.org/10.3892/or.2016.4992
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