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Article

Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells

  • Authors:
    • Hung-Sheng Shang
    • Chuan-Hsun Chang
    • Yu-Ru Chou
    • Ming-Yang Yeh
    • Man-Kuan Au
    • Hsu-Feng Lu
    • Yung-Lin Chu
    • Hsiao-Min Chou
    • Hsiu-Chen Chou
    • Yung-Luen Shih
    • Jing-Gung Chung
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, National Defense Medical Center, Division of Clinical Pathology, Tri-Service General Hospital, Taipei, Taiwan, R.O.C., Department of Surgical Oncology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C., Department of Nutrition Therapy, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C., Department of Office of Director, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C., Department of Orthopedics, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C., Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C., International Master's Degree Program in Food Science, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan, R.O.C., Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
  • Pages: 2207-2215
    |
    Published online on: August 4, 2016
       https://doi.org/10.3892/or.2016.5002
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Abstract

Cervical cancer is one of the most common cancers in women worldwide and it is a prominent cause of cancer mortality. Curcumin is one of the major compounds from Turmeric and has been shown to induce cytotoxic cell death in human cervical cancer cells. However, there is no study to show curcumin induced DNA damage action via the effect on the DNA damage and repair protein in cervical cancer cells in detail. In this study, we investigated whether or not curcumin induced cell death via DNA damage, chromatin condensation in human cervical cancer HeLa cells by using comet assay and DAPI staining, respectively, we found that curcumin induced cell death through the induction of DNA damage, and chromatin condensation. Western blotting and confocal laser microscopy examination were used to examine the effects of curcumin on protein expression associated with DNA damage, repair and translocation of proteins. We found that curcumin at 13 µM increased the protein levels associated with DNA damage and repair, such as O6-methylguanine-DNA methyltransferase, early-onset breast cancer 1 (BRCA1), mediator of DNA damage checkpoint 1, p-p53 and p-H2A.XSer140 in HeLa cells. Results from confocal laser systems microscopy indicated that curcumin increased the translocation of p-p53 and p-H2A.XSer140 from cytosol to nuclei in HeLa cells. In conclusion, curcumin induced cell death in HeLa cells via induction of DNA damage, and chromatin condensation in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Shang H, Chang C, Chou Y, Yeh M, Au M, Lu H, Chu Y, Chou H, Chou H, Shih Y, Shih Y, et al: Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells. Oncol Rep 36: 2207-2215, 2016.
APA
Shang, H., Chang, C., Chou, Y., Yeh, M., Au, M., Lu, H. ... Chung, J. (2016). Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells. Oncology Reports, 36, 2207-2215. https://doi.org/10.3892/or.2016.5002
MLA
Shang, H., Chang, C., Chou, Y., Yeh, M., Au, M., Lu, H., Chu, Y., Chou, H., Chou, H., Shih, Y., Chung, J."Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells". Oncology Reports 36.4 (2016): 2207-2215.
Chicago
Shang, H., Chang, C., Chou, Y., Yeh, M., Au, M., Lu, H., Chu, Y., Chou, H., Chou, H., Shih, Y., Chung, J."Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells". Oncology Reports 36, no. 4 (2016): 2207-2215. https://doi.org/10.3892/or.2016.5002
Copy and paste a formatted citation
x
Spandidos Publications style
Shang H, Chang C, Chou Y, Yeh M, Au M, Lu H, Chu Y, Chou H, Chou H, Shih Y, Shih Y, et al: Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells. Oncol Rep 36: 2207-2215, 2016.
APA
Shang, H., Chang, C., Chou, Y., Yeh, M., Au, M., Lu, H. ... Chung, J. (2016). Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells. Oncology Reports, 36, 2207-2215. https://doi.org/10.3892/or.2016.5002
MLA
Shang, H., Chang, C., Chou, Y., Yeh, M., Au, M., Lu, H., Chu, Y., Chou, H., Chou, H., Shih, Y., Chung, J."Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells". Oncology Reports 36.4 (2016): 2207-2215.
Chicago
Shang, H., Chang, C., Chou, Y., Yeh, M., Au, M., Lu, H., Chu, Y., Chou, H., Chou, H., Shih, Y., Chung, J."Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells". Oncology Reports 36, no. 4 (2016): 2207-2215. https://doi.org/10.3892/or.2016.5002
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