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Article

RUNX3 plays an important role in As2O3‑induced apoptosis and allows cells to overcome MSC‑mediated drug resistance

  • Authors:
    • Guo‑Zheng Pan
    • Feng‑Xian Zhai
    • Yin Lu
    • Zhi‑Gang Fang
    • Rui‑Fang Fan
    • Xiang‑Fu Liu
    • Dong‑Jun Lin
  • View Affiliations / Copyright

    Affiliations: Renal Transplantation Center, Anhui Provincial Hospital, Hefei, Anhui 230001, P.R. China, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China, Department of Hematology, The Third Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510630, P.R. China
  • Pages: 1927-1938
    |
    Published online on: August 5, 2016
       https://doi.org/10.3892/or.2016.5005
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Abstract

The interaction between bone marrow stromal cells and leukemia cells is critical for the persistence and progression of leukemia, and this interaction may account for residual disease. However, the link between leukemia cells and their environment is still poorly understood. In our study, runt‑related transcription factor 3 (RUNX3) was identified as a novel target gene affected by As2O3 and involved in mesenchymal stem cell (MSC)‑mediated protection of leukemia cells from As2O3‑induced apoptosis. We observed induction of RUNX3 expression and the translocation of RUNX3 into the nucleus after As2O3 treatment in leukemia cells. In K562 chronic myeloid leukemia cells, downregulation of endogenous RUNX3 compromised As2O3‑induced growth inhibition, cell cycle arrest, and apoptosis. In the presence of MSC, As2O3‑induced expression of RUNX3 was reduced significantly and this reduction was modulated by CXCL12/CXCR4 signaling. Furthermore, overexpression of RUNX3 restored, at least in part, the sensitivity of leukemic cells to As2O3. We conclude that RUNX3 plays an important role in As2O3‑induced cellular responses and allows cells to overcome MSC‑mediated drug resistance. Therefore, RUNX3 is a promising target for therapeutic approaches to overcome MSC‑mediated drug resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Pan GZ, Zhai FX, Lu Y, Fang ZG, Fan RF, Liu XF and Lin DJ: RUNX3 plays an important role in As2O3‑induced apoptosis and allows cells to overcome MSC‑mediated drug resistance. Oncol Rep 36: 1927-1938, 2016.
APA
Pan, G., Zhai, F., Lu, Y., Fang, Z., Fan, R., Liu, X., & Lin, D. (2016). RUNX3 plays an important role in As2O3‑induced apoptosis and allows cells to overcome MSC‑mediated drug resistance. Oncology Reports, 36, 1927-1938. https://doi.org/10.3892/or.2016.5005
MLA
Pan, G., Zhai, F., Lu, Y., Fang, Z., Fan, R., Liu, X., Lin, D."RUNX3 plays an important role in As2O3‑induced apoptosis and allows cells to overcome MSC‑mediated drug resistance". Oncology Reports 36.4 (2016): 1927-1938.
Chicago
Pan, G., Zhai, F., Lu, Y., Fang, Z., Fan, R., Liu, X., Lin, D."RUNX3 plays an important role in As2O3‑induced apoptosis and allows cells to overcome MSC‑mediated drug resistance". Oncology Reports 36, no. 4 (2016): 1927-1938. https://doi.org/10.3892/or.2016.5005
Copy and paste a formatted citation
x
Spandidos Publications style
Pan GZ, Zhai FX, Lu Y, Fang ZG, Fan RF, Liu XF and Lin DJ: RUNX3 plays an important role in As2O3‑induced apoptosis and allows cells to overcome MSC‑mediated drug resistance. Oncol Rep 36: 1927-1938, 2016.
APA
Pan, G., Zhai, F., Lu, Y., Fang, Z., Fan, R., Liu, X., & Lin, D. (2016). RUNX3 plays an important role in As2O3‑induced apoptosis and allows cells to overcome MSC‑mediated drug resistance. Oncology Reports, 36, 1927-1938. https://doi.org/10.3892/or.2016.5005
MLA
Pan, G., Zhai, F., Lu, Y., Fang, Z., Fan, R., Liu, X., Lin, D."RUNX3 plays an important role in As2O3‑induced apoptosis and allows cells to overcome MSC‑mediated drug resistance". Oncology Reports 36.4 (2016): 1927-1938.
Chicago
Pan, G., Zhai, F., Lu, Y., Fang, Z., Fan, R., Liu, X., Lin, D."RUNX3 plays an important role in As2O3‑induced apoptosis and allows cells to overcome MSC‑mediated drug resistance". Oncology Reports 36, no. 4 (2016): 1927-1938. https://doi.org/10.3892/or.2016.5005
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