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Article

MicroRNA-106a suppresses proliferation, migration, and invasion of bladder cancer cells by modulating MAPK signaling, cell cycle regulators, and Ets-1-mediated MMP-2 expression

  • Authors:
    • Seung-Shick Shin
    • Sung-Soo Park
    • Byungdoo Hwang
    • Won Tae Kim
    • Yung Hyun Choi
    • Wun-Jae Kim
    • Sung-Kwon Moon
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Nutrition, Jeju National University, Jeju 63243, Republic of Korea, Department of Food and Nutrition, Chung-Ang University, Anseong 456-756, Republic of Korea, Department of Urology, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea, Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan 614-052, Republic of Korea
  • Pages: 2421-2429
    |
    Published online on: August 11, 2016
       https://doi.org/10.3892/or.2016.5015
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Abstract

Despite the clinical significance of tumorigenesis, little is known about the cellular signaling networks of microRNAs (miRs). Here we report a new finding that mir‑106a regulates the proliferation, migration, and invasion of bladder cancer cells. Basal expression levels of mir‑106a were significantly lower in bladder cancer cells than in normal urothelial cells. Overexpression of mir‑106a suppressed the proliferation of bladder cancer cell line EJ. Transient transfection of mir‑106a into EJ cells led to downregulation of ERK phosphorylation and upregulation of p38 and JNK phosphorylation over their levels in the control. Flow cytometry analysis revealed that mir‑106a-transfected cells accumulated in the G1-phase of the cell cycle, and cyclin D1 and CDK6 were significantly downregulated. This G1-phase cell cycle arrest was due in part to the upregulation of p21CIP1/WAF1. In addition, mir‑106a overexpression blocked the wound-healing migration and invasion of EJ cells. Furthermore, mir‑106a transfection resulted in decreased expression of MMP-2 and diminished binding activity of transcription factor Ets-1 in EJ cells. Collectively, we report the novel mir‑106a-mediated molecular signaling networks that regulate the proliferation, migration, and invasion of bladder cancer cells, suggesting that mir‑106a may be a therapeutic target for treating advanced bladder tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Shin S, Park S, Hwang B, Kim WT, Choi YH, Kim W and Moon S: MicroRNA-106a suppresses proliferation, migration, and invasion of bladder cancer cells by modulating MAPK signaling, cell cycle regulators, and Ets-1-mediated MMP-2 expression. Oncol Rep 36: 2421-2429, 2016.
APA
Shin, S., Park, S., Hwang, B., Kim, W.T., Choi, Y.H., Kim, W., & Moon, S. (2016). MicroRNA-106a suppresses proliferation, migration, and invasion of bladder cancer cells by modulating MAPK signaling, cell cycle regulators, and Ets-1-mediated MMP-2 expression. Oncology Reports, 36, 2421-2429. https://doi.org/10.3892/or.2016.5015
MLA
Shin, S., Park, S., Hwang, B., Kim, W. T., Choi, Y. H., Kim, W., Moon, S."MicroRNA-106a suppresses proliferation, migration, and invasion of bladder cancer cells by modulating MAPK signaling, cell cycle regulators, and Ets-1-mediated MMP-2 expression". Oncology Reports 36.4 (2016): 2421-2429.
Chicago
Shin, S., Park, S., Hwang, B., Kim, W. T., Choi, Y. H., Kim, W., Moon, S."MicroRNA-106a suppresses proliferation, migration, and invasion of bladder cancer cells by modulating MAPK signaling, cell cycle regulators, and Ets-1-mediated MMP-2 expression". Oncology Reports 36, no. 4 (2016): 2421-2429. https://doi.org/10.3892/or.2016.5015
Copy and paste a formatted citation
x
Spandidos Publications style
Shin S, Park S, Hwang B, Kim WT, Choi YH, Kim W and Moon S: MicroRNA-106a suppresses proliferation, migration, and invasion of bladder cancer cells by modulating MAPK signaling, cell cycle regulators, and Ets-1-mediated MMP-2 expression. Oncol Rep 36: 2421-2429, 2016.
APA
Shin, S., Park, S., Hwang, B., Kim, W.T., Choi, Y.H., Kim, W., & Moon, S. (2016). MicroRNA-106a suppresses proliferation, migration, and invasion of bladder cancer cells by modulating MAPK signaling, cell cycle regulators, and Ets-1-mediated MMP-2 expression. Oncology Reports, 36, 2421-2429. https://doi.org/10.3892/or.2016.5015
MLA
Shin, S., Park, S., Hwang, B., Kim, W. T., Choi, Y. H., Kim, W., Moon, S."MicroRNA-106a suppresses proliferation, migration, and invasion of bladder cancer cells by modulating MAPK signaling, cell cycle regulators, and Ets-1-mediated MMP-2 expression". Oncology Reports 36.4 (2016): 2421-2429.
Chicago
Shin, S., Park, S., Hwang, B., Kim, W. T., Choi, Y. H., Kim, W., Moon, S."MicroRNA-106a suppresses proliferation, migration, and invasion of bladder cancer cells by modulating MAPK signaling, cell cycle regulators, and Ets-1-mediated MMP-2 expression". Oncology Reports 36, no. 4 (2016): 2421-2429. https://doi.org/10.3892/or.2016.5015
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