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Article

Foxp3 downregulation in NSCLC mediates epithelial-mesenchymal transition via NF-κB signaling

  • Authors:
    • Xi Wang
    • Ying Liu
    • Lingling Dai
    • Qi Liu
    • Liuqun Jia
    • Huan Wang
    • Lin An
    • Xiaogang Jing
    • Meng Liu
    • Pengfei Li
    • Zhe Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Disease, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China
  • Pages: 2282-2288
    |
    Published online on: August 17, 2016
       https://doi.org/10.3892/or.2016.5024
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Abstract

Forkhead box P3 (Foxp3) is a member of forkhead box transcription factor family and it was identified as a tumor suppressor in various solid tumors. This study evaluated the expression of Foxp3 in non-small cell lung cancer (NSCLC) and investigated its role in epithelial‑mesenchymal transition (EMT) of cancer cells. qRT-PCR and western blot analysis were used for examining the expression of Foxp3 in NSCLC tissues and the non-tumor tissues. A tissue microarray was constructed and scored for evaluating the clinical significance of Foxp3 expression in NSCLC tissues. RNAi was employed for downregulating Foxp3 expression and cell proliferation was done with MTT assay. Transwell with or without basement membrane matrix was used for cell migration and invasion assay respectively. Foxp3 was found downregulated in NSCLC tissues compared with non-tumoral tissues; downregulation of Foxp3 predicted adverse tumor stage and overall survival; silencing of FOXP3 promoted the proliferation, migration and invasion ability of NSCLC cells and influenced the expression level of EMT-associated proteins. However, forced expression of Foxp3 could reverse this effect. Moreover, Foxp3 could interact with LMO2 and affect the expression level of TAL1, which was in accordance with the findings in T-cell acute lymphoblastic leukemia. By screening the signalling pathways, we observed an obvious upregulation of phosphorylated NF-κB in A549 and H520 cells after silencing of FOXP3. Our results suggest that Foxp3 suppressed NSCLC cell metastasis, at least partially, via NF-κB signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Liu Y, Dai L, Liu Q, Jia L, Wang H, An L, Jing X, Liu M, Li P, Li P, et al: Foxp3 downregulation in NSCLC mediates epithelial-mesenchymal transition via NF-κB signaling. Oncol Rep 36: 2282-2288, 2016.
APA
Wang, X., Liu, Y., Dai, L., Liu, Q., Jia, L., Wang, H. ... Cheng, Z. (2016). Foxp3 downregulation in NSCLC mediates epithelial-mesenchymal transition via NF-κB signaling. Oncology Reports, 36, 2282-2288. https://doi.org/10.3892/or.2016.5024
MLA
Wang, X., Liu, Y., Dai, L., Liu, Q., Jia, L., Wang, H., An, L., Jing, X., Liu, M., Li, P., Cheng, Z."Foxp3 downregulation in NSCLC mediates epithelial-mesenchymal transition via NF-κB signaling". Oncology Reports 36.4 (2016): 2282-2288.
Chicago
Wang, X., Liu, Y., Dai, L., Liu, Q., Jia, L., Wang, H., An, L., Jing, X., Liu, M., Li, P., Cheng, Z."Foxp3 downregulation in NSCLC mediates epithelial-mesenchymal transition via NF-κB signaling". Oncology Reports 36, no. 4 (2016): 2282-2288. https://doi.org/10.3892/or.2016.5024
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Liu Y, Dai L, Liu Q, Jia L, Wang H, An L, Jing X, Liu M, Li P, Li P, et al: Foxp3 downregulation in NSCLC mediates epithelial-mesenchymal transition via NF-κB signaling. Oncol Rep 36: 2282-2288, 2016.
APA
Wang, X., Liu, Y., Dai, L., Liu, Q., Jia, L., Wang, H. ... Cheng, Z. (2016). Foxp3 downregulation in NSCLC mediates epithelial-mesenchymal transition via NF-κB signaling. Oncology Reports, 36, 2282-2288. https://doi.org/10.3892/or.2016.5024
MLA
Wang, X., Liu, Y., Dai, L., Liu, Q., Jia, L., Wang, H., An, L., Jing, X., Liu, M., Li, P., Cheng, Z."Foxp3 downregulation in NSCLC mediates epithelial-mesenchymal transition via NF-κB signaling". Oncology Reports 36.4 (2016): 2282-2288.
Chicago
Wang, X., Liu, Y., Dai, L., Liu, Q., Jia, L., Wang, H., An, L., Jing, X., Liu, M., Li, P., Cheng, Z."Foxp3 downregulation in NSCLC mediates epithelial-mesenchymal transition via NF-κB signaling". Oncology Reports 36, no. 4 (2016): 2282-2288. https://doi.org/10.3892/or.2016.5024
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