Casticin induces DNA damage and inhibits DNA repair-associated protein expression in B16F10 mouse melanoma cancer cells

  • Authors:
    • Yung-Luen Shih
    • Jason Chou
    • Ming-Yang Yeh
    • Hsiao-Min Chou
    • Hsiu-Chen Chou
    • Hsu-Feng Lu
    • Hung-Sheng Shang
    • Fu-Shin Chueh
    • Yung-Lin Chu
    • Shu-Ching Hsueh
    • Jing-Gung Chung
  • View Affiliations

  • Published online on: August 17, 2016     https://doi.org/10.3892/or.2016.5027
  • Pages: 2094-2100
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Abstract

Casticin, a polymethoxyflavone, has been demonstrated to possess anticancer activities, yet no study has shown in detail that casticin induces DNA damage in lung cancer cells. The purpose of this study was to investigate the possible molecular mechanisms of casticin which induce DNA damage and nuclear condensation in murine melanoma cancer B16F10 cells. In this study, by examining and capturing images using phase contrast microscopy, we found that casticin induced cell morphological changes. Moreover, it decreased the total number of viable cells which was measured by flow cytometry. Casticin-induced DNA damage and nuclear DNA condensation were measured by DAPI staining, respectively. Western blotting indicated that casticin decreased the protein levels of O6‑methylguanine-DNA methyltransferase (MGMT), breast cancer 1, early onset (BRCA1), mediator of DNA damage checkpoint 1 (MDC1), DNA-dependent protein kinase (DNA-PK) but increased phospho-p53 tumor suppressor protein (p-p53), phospho-ataxia telangiectasia mutated kinase (p-ATM), phospho-histone H2A.X (Ser139) and poly(ADP-ribose) polymerase (PARP) in the B16F10 cells. Furthermore, we used confocal laser system microscopy to examine the protein expression levels and we found that casticin increased the expression of p-p53 and p-H2A.X in the B16F10 cells. Collectively, casticin induced DNA damage and affected DNA repair proteins in the B16F10 cells in vitro.
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October-2016
Volume 36 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Shih Y, Chou J, Yeh M, Chou H, Chou H, Lu H, Shang H, Chueh F, Chu Y, Hsueh S, Hsueh S, et al: Casticin induces DNA damage and inhibits DNA repair-associated protein expression in B16F10 mouse melanoma cancer cells. Oncol Rep 36: 2094-2100, 2016
APA
Shih, Y., Chou, J., Yeh, M., Chou, H., Chou, H., Lu, H. ... Chung, J. (2016). Casticin induces DNA damage and inhibits DNA repair-associated protein expression in B16F10 mouse melanoma cancer cells. Oncology Reports, 36, 2094-2100. https://doi.org/10.3892/or.2016.5027
MLA
Shih, Y., Chou, J., Yeh, M., Chou, H., Chou, H., Lu, H., Shang, H., Chueh, F., Chu, Y., Hsueh, S., Chung, J."Casticin induces DNA damage and inhibits DNA repair-associated protein expression in B16F10 mouse melanoma cancer cells". Oncology Reports 36.4 (2016): 2094-2100.
Chicago
Shih, Y., Chou, J., Yeh, M., Chou, H., Chou, H., Lu, H., Shang, H., Chueh, F., Chu, Y., Hsueh, S., Chung, J."Casticin induces DNA damage and inhibits DNA repair-associated protein expression in B16F10 mouse melanoma cancer cells". Oncology Reports 36, no. 4 (2016): 2094-2100. https://doi.org/10.3892/or.2016.5027