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Article

Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer

  • Authors:
    • Heyan Zhao
    • Jianhua Xue
    • Junhua Liu
    • Yifei Liu
    • Yinan Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Emergency Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
  • Pages: 2814-2822
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    Published online on: September 12, 2016
       https://doi.org/10.3892/or.2016.5081
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Abstract

The present study aimed to investigate the effect of metastasis suppressor 1 (MTSS1) on the proliferation, migration and invasion of human H1299 non-small cell lung cancer cells and its clinical significance in non‑small cell lung cancer. The target gene MTSS1-overexpressing lentivirus (LV-MTSS1) was transfected into H1299 cells and expression of MTSS1 was detected at the mRNA and protein levels. Cell Counting Kit-8, wound healing and Transwell assays revealed that the migration and invasion activities were significantly suppressed by MTSS1, but that it had no effect on cell proliferation. In addition, MTSS1 expression in tissue microarrays including samples from 223 cases of non-small cell lung cancer was tested by immunohistochemistry to explore the correlation between MTSS1 and clinicopathological characteristics and prognosis. MTSS1 suppressed H1299 cell migration and invasion, and its expression level can be used as a new independent factor for determining the prognosis of non-small cell lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao H, Xue J, Liu J, Liu Y and Cheng Y: Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer. Oncol Rep 36: 2814-2822, 2016.
APA
Zhao, H., Xue, J., Liu, J., Liu, Y., & Cheng, Y. (2016). Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer. Oncology Reports, 36, 2814-2822. https://doi.org/10.3892/or.2016.5081
MLA
Zhao, H., Xue, J., Liu, J., Liu, Y., Cheng, Y."Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer". Oncology Reports 36.5 (2016): 2814-2822.
Chicago
Zhao, H., Xue, J., Liu, J., Liu, Y., Cheng, Y."Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer". Oncology Reports 36, no. 5 (2016): 2814-2822. https://doi.org/10.3892/or.2016.5081
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao H, Xue J, Liu J, Liu Y and Cheng Y: Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer. Oncol Rep 36: 2814-2822, 2016.
APA
Zhao, H., Xue, J., Liu, J., Liu, Y., & Cheng, Y. (2016). Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer. Oncology Reports, 36, 2814-2822. https://doi.org/10.3892/or.2016.5081
MLA
Zhao, H., Xue, J., Liu, J., Liu, Y., Cheng, Y."Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer". Oncology Reports 36.5 (2016): 2814-2822.
Chicago
Zhao, H., Xue, J., Liu, J., Liu, Y., Cheng, Y."Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer". Oncology Reports 36, no. 5 (2016): 2814-2822. https://doi.org/10.3892/or.2016.5081
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