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Article

Establishment and characterization of an expanding-type gastric cancer cell line by Ming's classification

  • Authors:
    • Hao Xu
    • Ji-Gui Peng
    • Yi-Fan Zhuang
    • Jia-Jia Chen
    • Qi-Cong Luo
    • Wei-Feng Huang
    • Chun-Dong Lin
    • Jian-Chun Cai
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian 361005, P.R. China, Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, Fujian 361005, P.R. China
  • Pages: 3030-3036
    |
    Published online on: September 14, 2016
       https://doi.org/10.3892/or.2016.5090
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Abstract

According to Ming's classification, gastric cancer (GC) can be divided into two types: expanding and infiltrative. The two types are readily recognizable by histology: expanding carcinomas grow en masse and by expansion, resulting in the formation of discrete tumour nodules, whereas in infiltrative carcinoma, tumour cells invade individually. Both types show varying degrees of cell maturation. The two types of carcinomas have vastly different pathological and clinical features. However, little is known concerning the mechanisms underlying these differences since no GC cell line models are available. For comprehensive and insightful analyses of mechanisms and treatment methods, new cell lines derived from expanding- and infiltrative-type gastric tumours are urgently needed. In the present study, we established an expanding-type GC cell line from a 72-year-old male patient. Different in vitro and in vivo methods were used to characterize the phenotypes of this cell line. This GC cell line was named XGC-2 and had an ~60 h doubling time. The cell line displayed strong colony formation and tumourigenicity in nude mice and had complicated chromosomal abnormalities. XGC-2 cells showed some markers of epithelial-to-mesenchymal transition (EMT), with decreased E-cadherin expression levels and increased vimentin expression levels. The XGC-2 cell line may be useful for future studies of GC development, progression, metastasis and therapy.
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1 

Wadhwa R, Song S, Lee JS, Yao Y, Wei Q and Ajani JA: Gastric cancer-molecular and clinical dimensions. Nat Rev Clin Oncol. 10:643–655. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Bertuccio P, Chatenoud L, Levi F, Praud D, Ferlay J, Negri E, Malvezzi M and La Vecchia C: Recent patterns in gastric cancer: A global overview. Int J Cancer. 125:666–673. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Chen J, Bu XL, Wang QY, Hu PJ and Chen MH: Decreasing seroprevalence of Helicobacter pylori infection during 1993–2003 in Guangzhou, southern China. Helicobacter. 12:164–169. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Kawakami E, Machado RS, Ogata SK and Langner M: Decrease in prevalence of Helicobacter pylori infection during a 10-year period in Brazilian children. Arq Gastroenterol. 45:147–151. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Meyerhardt JA and Fuchs CS: Adjuvant therapy in gastric cancer: Can we prevent recurrences? Oncology. 17:714–733. 2003.PubMed/NCBI

7 

Gey GO, Coffman WD and Kubicek MT: Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12:264–265. 1952.

8 

Barranco SC, Townsend CM Jr, Casartelli C, Macik BG, Burger NL, Boerwinkle WR and Gourley WK: Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach. Cancer Res. 43:1703–1709. 1983.PubMed/NCBI

9 

Motoyama T, Hojo H and Watanabe H: Comparison of seven cell lines derived from human gastric carcinomas. Acta Pathol Jpn. 36:65–83. 1986.PubMed/NCBI

10 

Park JG, Frucht H, LaRocca RV, Bliss DP Jr, Kurita Y, Chen TR, Henslee JG, Trepel JB, Jensen RT, Johnson BE, et al: Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50:2773–2780. 1990.PubMed/NCBI

11 

Park JG, Yang HK, Kim WH, Chung JK, Kang MS, Lee JH, Oh JH, Park HS, Yeo KS, Kang SH, et al: Establishment and characterization of human gastric carcinoma cell lines. Int J Cancer. 70:443–449. 1997. View Article : Google Scholar : PubMed/NCBI

12 

Ku JL, Kim KH, Choi JS, Kim SH, Shin YK, Chang HJ, Bae JM, Kim YW, Lee JH, Yang HK, et al: Establishment and characterization of six human gastric carcinoma cell lines, including one naturally infected with Epstein-Barr virus. Cell Oncol. 35:127–136. 2012. View Article : Google Scholar

13 

Akiyama S, Amo H, Watanabe T, Matsuyama M, Sakamoto J, Imaizumi M, Ichihashi H, Kondo T and Takagi H: Characteristics of three human gastric cancer cell lines, NU-GC-2, NU-GC-3 and NU-GC-4. Jpn J Surg. 18:438–446. 1988. View Article : Google Scholar : PubMed/NCBI

14 

Holliday DL and Speirs V: Choosing the right cell line for breast cancer research. Breast Cancer Res. 13:2152011. View Article : Google Scholar : PubMed/NCBI

15 

Burdall SE, Hanby AM, Lansdown MR and Speirs V: Breast cancer cell lines: Friend or foe? Breast Cancer Res. 5:89–95. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Osborne CK, Hobbs K and Trent JM: Biological differences among MCF-7 human breast cancer cell lines from different laboratories. Breast Cancer Res Treat. 9:111–121. 1987. View Article : Google Scholar : PubMed/NCBI

17 

Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, et al: Check your cultures! A list of cross-contaminated or misidentified cell lines. Int J Cancer. 127:1–8. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Christgen M and Lehmann U: MDA-MB-435: The questionable use of a melanoma cell line as a model for human breast cancer is ongoing. Cancer Biol Ther. 6:1355–1357. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Cheung PF, Yip CW, Ng LW, Lo KW, Wong N, Choy KW, Chow C, Chan KF, Cheung TT, Poon RT, et al: Establishment and characterization of a novel primary hepatocellular carcinoma cell line with metastatic ability in vivo. Cancer Cell Int. 14:1032014. View Article : Google Scholar : PubMed/NCBI

20 

Wang JH, Li LF, Yu Y, Li B, Jin HJ, Shen DH, Li J, Jiang XQ and Qian QJ: Establishment and characterization of a cell line, EH-GB2, derived from hepatic metastasis of gallbladder cancer. Oncol Rep. 27:775–782. 2012.

21 

Ming SC: Gastric carcinoma. A pathobiological classification. Cancer. 39:2475–2485. 1977. View Article : Google Scholar : PubMed/NCBI

22 

Ma S, Hu L, Huang XH, Cao LQ, Chan KW, Wang Q and Guan XY: Establishment and characterization of a human cholangiocarcinoma cell line. Oncol Rep. 18:1195–1200. 2007.PubMed/NCBI

23 

Limpaiboon T, Tapdara S, Jearanaikoon P, Sripa B and Bhudhisawasdi V: Prognostic significance of microsatellite alterations at 1p36 in cholangiocarcinoma. World J Gastroenterol. 12:4377–4382. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Uhm KO, Park YN, Lee JY, Yoon DS and Park SH: Chromosomal imbalances in Korean intrahepatic cholangiocarcinoma by comparative genomic hybridization. Cancer Genet Cytogenet. 157:37–41. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Wada M, Yokota J, Mizoguchi H, Terada M and Sugimura T: Y chromosome abnormality in human stomach and lung cancerJpn. J Cancer Res. 78:780–783. 1987.

26 

Yanagihara K, Seyama T, Tsumuraya M, Kamada N and Yokoro K: Establishment and characterization of human signet ring cell gastric carcinoma cell lines with amplification of the c-myc oncogene. Cancer Res. 51:381–386. 1991.PubMed/NCBI

27 

Geiger TR and Peeper DS: Metastasis mechanisms. Biochim Biophys Acta. 1796:293–308. 2009.PubMed/NCBI

28 

Boyer B, Vallés AM and Edme N: Induction and regulation of epithelial-mesenchymal transitions. Biochem Pharmacol. 60:1091–1099. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Kudo-Saito C, Shirako H, Takeuchi T and Kawakami Y: Cancer metastasis is accelerated through immunosuppression during Snail-induced EMT of cancer cells. Cancer Cell. 15:195–206. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Batlle E, Sancho E, Francí C, Domínguez D, Monfar M, Baulida J and García De Herreros A: The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells. Nat Cell Biol. 2:84–89. 2000. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu H, Peng J, Zhuang Y, Chen J, Luo Q, Huang W, Lin C and Cai J: Establishment and characterization of an expanding-type gastric cancer cell line by Ming's classification. Oncol Rep 36: 3030-3036, 2016.
APA
Xu, H., Peng, J., Zhuang, Y., Chen, J., Luo, Q., Huang, W. ... Cai, J. (2016). Establishment and characterization of an expanding-type gastric cancer cell line by Ming's classification. Oncology Reports, 36, 3030-3036. https://doi.org/10.3892/or.2016.5090
MLA
Xu, H., Peng, J., Zhuang, Y., Chen, J., Luo, Q., Huang, W., Lin, C., Cai, J."Establishment and characterization of an expanding-type gastric cancer cell line by Ming's classification". Oncology Reports 36.5 (2016): 3030-3036.
Chicago
Xu, H., Peng, J., Zhuang, Y., Chen, J., Luo, Q., Huang, W., Lin, C., Cai, J."Establishment and characterization of an expanding-type gastric cancer cell line by Ming's classification". Oncology Reports 36, no. 5 (2016): 3030-3036. https://doi.org/10.3892/or.2016.5090
Copy and paste a formatted citation
x
Spandidos Publications style
Xu H, Peng J, Zhuang Y, Chen J, Luo Q, Huang W, Lin C and Cai J: Establishment and characterization of an expanding-type gastric cancer cell line by Ming's classification. Oncol Rep 36: 3030-3036, 2016.
APA
Xu, H., Peng, J., Zhuang, Y., Chen, J., Luo, Q., Huang, W. ... Cai, J. (2016). Establishment and characterization of an expanding-type gastric cancer cell line by Ming's classification. Oncology Reports, 36, 3030-3036. https://doi.org/10.3892/or.2016.5090
MLA
Xu, H., Peng, J., Zhuang, Y., Chen, J., Luo, Q., Huang, W., Lin, C., Cai, J."Establishment and characterization of an expanding-type gastric cancer cell line by Ming's classification". Oncology Reports 36.5 (2016): 3030-3036.
Chicago
Xu, H., Peng, J., Zhuang, Y., Chen, J., Luo, Q., Huang, W., Lin, C., Cai, J."Establishment and characterization of an expanding-type gastric cancer cell line by Ming's classification". Oncology Reports 36, no. 5 (2016): 3030-3036. https://doi.org/10.3892/or.2016.5090
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