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Article

Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products

  • Authors:
    • Junbo Li
    • Juan Ma
    • Ke Si Wang
    • Chunliu Mi
    • Zhe Wang
    • Lian Xun Piao
    • Guang Hua Xu
    • Xuezheng Li
    • Jung Joon Lee
    • Xuejun Jin
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Molecular Cancer Research Center, College of Pharmacy, Yanbian University, Yanji, Jilin 133002, P.R. China
  • Pages: 2771-2776
    |
    Published online on: September 19, 2016
       https://doi.org/10.3892/or.2016.5108
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Abstract

The nuclear factor-κB (NF-κB) transcription factors control many physiological processes including inflammation, immunity, apoptosis and angiogenesis. In our search for NF-κB inhibitors from natural resources, we identified baicalein from Scutellaria baicalensis as an inhibitor of NF-κB activation. As examined by the NF-κB luciferase reporter assay, we found that baicalein suppressed TNF-α-induced NF-κB activation in a dose-dependent manner. It also inhibited TNF-α-induced nuclear translocation of p65 through inhibition of phosphorylation and degradation of IκBα. Furthermore, baicalein blocked the TNF-α-induced expression of NF-κB target genes involved in anti-apoptosis (cIAP-1, cIAP-2, FLIP and BCL-2), proliferation (COX-2, cyclin D1 and c-Myc), invasion (MMP‑9), angiogenesis (VEGF) and major inflammatory cytokines (IL-8 and MCP1). The flow cytometric analysis indicated that baicalein potentiated TNF-α-induced apoptosis and induced G1 phase arrest in HeLa cells. Moreover, baicalein significantly blocked activation of p38, extracellular signal-regulated kinase 1/2 (ERK1/2). Our results imply that baicalein could be a lead compound for the modulation of inflammatory diseases as well as certain cancers in which inhibition of NF-κB activity may be desirable.
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1 

Sen R and Baltimore D: Multiple nuclear factors interact with the immunoglobulin enhancer sequences. Cell. 46:705–716. 1986. View Article : Google Scholar : PubMed/NCBI

2 

Aggarwal BB: Nuclear factor-kappaB: The enemy within. Cancer Cell. 6:203–208. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Shi H, Ma J, Mi C, Li J, Wang F, Lee JJ and Jin X: Amorfrutin A inhibits TNF-α-induced NF-κB activation and NF-κB-regulated target gene products. Int Immunopharmacol. 21:56–62. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Li M, Song S, Li S, Feng J and Hua Z: The Blockade of NF-κB activation by a specific inhibitory peptide has a strong neuroprotective role in a Sprague-Dawley rat kernicterus model. J Biol Chem. 290:30042–30052. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Karin M and Ben-Neriah Y: Phosphorylation meets ubiquitination: The control of NF-[kappa]B activity. Annu Rev Immunol. 18:621–663. 2000. View Article : Google Scholar

6 

Sethi G, Sung B and Aggarwal BB: Nuclear factor-kappaB activation: From bench to bedside. Exp Biol Med (Maywood). 233:21–31. 2008. View Article : Google Scholar

7 

Wang Y, Han E, Xing Q, Yan J, Arrington A, Wang C, Tully D, Kowolik CM, Lu DM, Frankel PH, et al: Baicalein upregulates DDIT4 expression which mediates mTOR inhibition and growth inhibition in cancer cells. Cancer Lett. 358:170–179. 2015. View Article : Google Scholar

8 

Li-Weber M: New therapeutic aspects of flavones: The anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin. Cancer Treat Rev. 35:57–68. 2009. View Article : Google Scholar

9 

Ku SK and Bae JS: Baicalin, baicalein and wogonin inhibits high glucose-induced vascular inflammation in vitro and in vivo. BMB Rep. 48:519–524. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Ma GZ, Liu CH, Wei B, Qiao J, Lu T, Wei HC, Chen HD and He CD: Baicalein inhibits DMBA/TPA-induced skin tumorigenesis in mice by modulating proliferation, apoptosis, and inflammation. Inflammation. 36:457–467. 2013. View Article : Google Scholar

11 

Shieh DE, Liu LT and Lin CC: Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin. Anticancer Res. 20(5A): 2861–2865. 2000.PubMed/NCBI

12 

Hwangbo C, Kim J, Lee JJ and Lee JH: Activation of the integrin effector kinase focal adhesion kinase in cancer cells is regulated by crosstalk between protein kinase Calpha and the PDZ adapter protein mda-9/Syntenin. Cancer Res. 70:1645–1655. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Jin HR, Jin SZ, Cai XF, Li D, Wu X, Nan JX, Lee JJ and Jin X: Cryptopleurine targets NF-κB pathway, leading to inhibition of gene products associated with cell survival, proliferation, invasion, and angiogenesis. PLoS One. 7:e403552012. View Article : Google Scholar

14 

Jin X, Jin HR, Jung HS, Lee SJ, Lee JH and Lee JJ: An atypical E3 ligase zinc finger protein 91 stabilizes and activates NF-kappaB-inducing kinase via Lys63-linked ubiquitination. J Biol Chem. 285:30539–30547. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Huxford T, Huang DB, Malek S and Ghosh G: The crystal structure of the IkappaBalpha/NF-kappaB complex reveals mechanisms of NF-kappaB inactivation. Cell. 95:759–770. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Napetschnig J and Wu H: Molecular basis of NF-κB signaling. Annu Rev Biophys. 42:443–468. 2013. View Article : Google Scholar :

17 

Jacobs MD and Harrison SC: Structure of an IkappaBalpha/NF-kappaB complex. Cell. 95:749–758. 1998. View Article : Google Scholar : PubMed/NCBI

18 

Lee SY, Cho JS, Yuk DY, Moon DC, Jung JK, Yoo HS, Lee YM, Han SB, Oh KW and Hong JT: Obovatol enhances docetaxel-induced prostate and colon cancer cell death through inactivation of nuclear transcription factor-kappaB. J Pharmacol Sci. 111:124–136. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Hunter AM, LaCasse EC and Korneluk RG: The inhibitors of apoptosis (IAPs) as cancer targets. Apoptosis. 12:1543–1568. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Zeelenberg IS, Ruuls-Van Stalle L and Roos E: The chemokine receptor CXCR4 is required for outgrowth of colon carcinoma micrometastases. Cancer Res. 63:3833–3839. 2003.PubMed/NCBI

21 

Alexiou D, Karayiannakis AJ, Syrigos KN, Zbar A, Sekara E, Michail P, Rosenberg T and Diamantis T: Clinical significance of serum levels of E-selectin, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 in gastric cancer patients. Am J Gastroenterol. 98:478–485. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Kuniyasu H1, Troncoso P, Johnston D, Bucana CD, Tahara E, Fidler IJ and Pettaway CA: Relative expression of type IV collagenase, E-cadherin, and vascular endothelial growth factor/vascular permeability factor in prostatectomy specimens distinguishes organ-confined from pathologically advanced prostate cancers. Clin Cancer Res. 6:2295–2308. 2000.PubMed/NCBI

23 

Vanden Berghe W, Plaisance S, Boone E, De Bosscher K, Schmitz ML, Fiers W and Haegeman G: p38 and extracellular signal-regulated kinase mitogen-activated protein kinase pathways are required for nuclear factor-kappaB p65 transactivation mediated by tumor necrosis factor. J Biol Chem. 273:3285–3290. 1998. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li J, Ma J, Wang KS, Mi C, Wang Z, Piao LX, Xu GH, Li X, Lee JJ, Jin X, Jin X, et al: Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products. Oncol Rep 36: 2771-2776, 2016.
APA
Li, J., Ma, J., Wang, K.S., Mi, C., Wang, Z., Piao, L.X. ... Jin, X. (2016). Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products. Oncology Reports, 36, 2771-2776. https://doi.org/10.3892/or.2016.5108
MLA
Li, J., Ma, J., Wang, K. S., Mi, C., Wang, Z., Piao, L. X., Xu, G. H., Li, X., Lee, J. J., Jin, X."Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products". Oncology Reports 36.5 (2016): 2771-2776.
Chicago
Li, J., Ma, J., Wang, K. S., Mi, C., Wang, Z., Piao, L. X., Xu, G. H., Li, X., Lee, J. J., Jin, X."Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products". Oncology Reports 36, no. 5 (2016): 2771-2776. https://doi.org/10.3892/or.2016.5108
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Ma J, Wang KS, Mi C, Wang Z, Piao LX, Xu GH, Li X, Lee JJ, Jin X, Jin X, et al: Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products. Oncol Rep 36: 2771-2776, 2016.
APA
Li, J., Ma, J., Wang, K.S., Mi, C., Wang, Z., Piao, L.X. ... Jin, X. (2016). Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products. Oncology Reports, 36, 2771-2776. https://doi.org/10.3892/or.2016.5108
MLA
Li, J., Ma, J., Wang, K. S., Mi, C., Wang, Z., Piao, L. X., Xu, G. H., Li, X., Lee, J. J., Jin, X."Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products". Oncology Reports 36.5 (2016): 2771-2776.
Chicago
Li, J., Ma, J., Wang, K. S., Mi, C., Wang, Z., Piao, L. X., Xu, G. H., Li, X., Lee, J. J., Jin, X."Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products". Oncology Reports 36, no. 5 (2016): 2771-2776. https://doi.org/10.3892/or.2016.5108
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