MicroRNA miR-374, a potential radiosensitizer for carbon ion beam radiotherapy

Baek,Sung-Jae; Sato,Katsutoshi; Nishida,Naohiro; Koseki,Jun; Azuma,Rikako; Kawamoto,Koichi; Konno,Masamitsu; Hayashi,Kazuhiko; Satoh,Taroh; Doki,Yuichiro; Mori,Masaki; Ishii,Hideshi; Ogawa,Kazuhiko

doi:10.3892/or.2016.5122

November-2016 Volume 36 Issue 5

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November-2016 Volume 36 Issue 5

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Article

MicroRNA miR-374, a potential radiosensitizer for carbon ion beam radiotherapy

Authors:
- Sung-Jae Baek
- Katsutoshi Sato
- Naohiro Nishida
- Jun Koseki
- Rikako Azuma
- Koichi Kawamoto
- Masamitsu Konno
- Kazuhiko Hayashi
- Taroh Satoh
- Yuichiro Doki
- Masaki Mori
- Hideshi Ishii
- Kazuhiko Ogawa
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Affiliations: Department of Radiation Oncology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Advanced Radiation Biology Research Program, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Inage, Chiba 263-8555, Japan, Department of Frontier Science for Cancer and Chemotherapy, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Department of Cancer Profiling Discovery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan
Pages: 2946-2950
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Published online on: September 22, 2016

https://doi.org/10.3892/or.2016.5122
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Abstract

In this study, we compared the microRNA (miRNA) profiles of a control and X-ray- and carbon ion beam-resistant cells to identify miRNAs that can be used as radiosensitizers and biomarkers. Mouse squamous cell carcinoma line NR-S1, its X-ray-resistant derivative X60, and its carbon ion beam‑resistant derivative C30 were subjected to miRNA microarray analysis. Expression of miRNAs shown to be upregulated or downregulated in the microarray analysis was confirmed by qRT-PCR. Downregulated miRNAs were overexpressed in human pancreatic cancer cell lines PANC1 and MIA PaCa-2, and the resulting cells were tested for radiosensitivity using colony-forming and sphere-forming assays. Of 1,265 miRNAs analyzed, 4 were downregulated and 11 were upregulated in X-ray-resistant and carbon ion beam-resistant cells. Two of the downregulated miRNAs, miR-196 and miR-374, were selected for overexpression in PANC1 and MIA PaCa-2 cells. Overexpression of miR-374 sensitized PANC-1 and MIA PaCa-2 cells toward carbon ion beam radiation. miRNA miR-374 has the potential to be a new radiosensitizer for carbon ion beam radiotherapy and a new biomarker to determine the optimal treatment for cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

MicroRNA miR-374, a potential radiosensitizer for carbon ion beam radiotherapy

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