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Article

Salidroside induces apoptosis and autophagy in human colorectal cancer cells through inhibition of PI3K/Akt/mTOR pathway

  • Authors:
    • Xiang-Jun Fan
    • Yao Wang
    • Lei Wang
    • Mingyan Zhu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
  • Pages: 3559-3567
    |
    Published online on: September 30, 2016
       https://doi.org/10.3892/or.2016.5138
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Abstract

The role of salidroside in colon cancer remains unknown. Here we show that salidroside, a phenylpropanoid glycoside extracted from Rhodiola rosea, exhibited potent anti-proliferative properties in human colorectal cancer cells via inducing apoptosis and autophagy. We ascertained that salidroside exerts an inhibitory effect on the proliferation of human colorectal cancer cells in a dose-dependent manner. In addition, salidroside induced cell apoptosis, accompanied by an increase of chromatin condensation and nuclear fragmentation, and a decrease of Bcl-2/Bax protein expression ratio. We also found that salidroside induced autophagy, evidenced by increased LC3+ autophagic vacuoles, positive acridine orange-stained cells, enhanced conversion of LC3-I to LC3-II, and elevation of Beclin-1. Treatment with autophagy-specific inhibitors [3-methyladenine (3-MA) and bafilomycin A1 (BA)] enhanced salidroside-induced apoptosis, indicating that salidroside-mediated autophagy may protect HT29 cells from undergoing apoptotic cell death. Additionally, salidroside decreased the phosphorylation of PI3K, Akt and mTOR. Treatment with PI3K inhibitor LY294002 augmented the effects of salidroside on the expression of Akt and mTOR. These findings indicate that salidroside could suppress the PI3K/Akt/mTOR signaling pathways. This study may provide a rationale for future clinical application using salidroside as a chemotherapeutic agent for human colorectal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Fan X, Wang Y, Wang L and Zhu M: Salidroside induces apoptosis and autophagy in human colorectal cancer cells through inhibition of PI3K/Akt/mTOR pathway. Oncol Rep 36: 3559-3567, 2016.
APA
Fan, X., Wang, Y., Wang, L., & Zhu, M. (2016). Salidroside induces apoptosis and autophagy in human colorectal cancer cells through inhibition of PI3K/Akt/mTOR pathway. Oncology Reports, 36, 3559-3567. https://doi.org/10.3892/or.2016.5138
MLA
Fan, X., Wang, Y., Wang, L., Zhu, M."Salidroside induces apoptosis and autophagy in human colorectal cancer cells through inhibition of PI3K/Akt/mTOR pathway". Oncology Reports 36.6 (2016): 3559-3567.
Chicago
Fan, X., Wang, Y., Wang, L., Zhu, M."Salidroside induces apoptosis and autophagy in human colorectal cancer cells through inhibition of PI3K/Akt/mTOR pathway". Oncology Reports 36, no. 6 (2016): 3559-3567. https://doi.org/10.3892/or.2016.5138
Copy and paste a formatted citation
x
Spandidos Publications style
Fan X, Wang Y, Wang L and Zhu M: Salidroside induces apoptosis and autophagy in human colorectal cancer cells through inhibition of PI3K/Akt/mTOR pathway. Oncol Rep 36: 3559-3567, 2016.
APA
Fan, X., Wang, Y., Wang, L., & Zhu, M. (2016). Salidroside induces apoptosis and autophagy in human colorectal cancer cells through inhibition of PI3K/Akt/mTOR pathway. Oncology Reports, 36, 3559-3567. https://doi.org/10.3892/or.2016.5138
MLA
Fan, X., Wang, Y., Wang, L., Zhu, M."Salidroside induces apoptosis and autophagy in human colorectal cancer cells through inhibition of PI3K/Akt/mTOR pathway". Oncology Reports 36.6 (2016): 3559-3567.
Chicago
Fan, X., Wang, Y., Wang, L., Zhu, M."Salidroside induces apoptosis and autophagy in human colorectal cancer cells through inhibition of PI3K/Akt/mTOR pathway". Oncology Reports 36, no. 6 (2016): 3559-3567. https://doi.org/10.3892/or.2016.5138
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