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Article

Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells

  • Authors:
    • Peizhen Li
    • Jun Ying
    • Qingli Chang
    • Wen Zhu
    • Guangjian Yang
    • Teng Xu
    • Huiguang Yi
    • Ruowang Pan
    • Enyong Zhang
    • Xiaofeng Zeng
    • Chunxia Yan
    • Qiyu Bao
    • Shengbin Li
  • View Affiliations / Copyright

    Affiliations: School of Forensic Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710000, P.R. China, School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, 118 Hospital of PLA, Wenzhou, Zhejiang 325000, P.R. China
  • Pages: 3536-3544
    |
    Published online on: October 11, 2016
       https://doi.org/10.3892/or.2016.5162
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Abstract

We studied phycoerythrin (PE) in human SW480 tumor cells and the underlying molecular mechanisms of action. PE inhibited cell proliferation as evidenced by CCK-8 assay. The IC50 values of phycoerythrin were 48.2 and 27.4 µg/ml for 24 and 48 h of exposure, respectively. PE induced apoptosis and cell cycle arrest in SW480 cells as observed under electron microscopy and with flow cytometry. Apoptosis increased from 5.1 (controls) to 39.0% in 80.0 µg/ml PE-treated cells. Differences in protein expression were identified using proteomic techniques. Protein spots (1018±60 and 1010±60) were resolved in PE-treated and untreated group. Forty differential protein spots were analyzed with MALDI-TOF-MS, including GRP78 and NPM1. The expression as measured by qPCR and western blotting agreed with data from two-dimensional electrophoresis. GRP78, NPM1, MTHSP75, Ezrin and Annexin A2 were decreased and HSP60 was increased after PE treatment, indicating that PE may target multiple proteins to induce apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Li P, Ying J, Chang Q, Zhu W, Yang G, Xu T, Yi H, Pan R, Zhang E, Zeng X, Zeng X, et al: Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells. Oncol Rep 36: 3536-3544, 2016.
APA
Li, P., Ying, J., Chang, Q., Zhu, W., Yang, G., Xu, T. ... Li, S. (2016). Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells. Oncology Reports, 36, 3536-3544. https://doi.org/10.3892/or.2016.5162
MLA
Li, P., Ying, J., Chang, Q., Zhu, W., Yang, G., Xu, T., Yi, H., Pan, R., Zhang, E., Zeng, X., Yan, C., Bao, Q., Li, S."Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells". Oncology Reports 36.6 (2016): 3536-3544.
Chicago
Li, P., Ying, J., Chang, Q., Zhu, W., Yang, G., Xu, T., Yi, H., Pan, R., Zhang, E., Zeng, X., Yan, C., Bao, Q., Li, S."Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells". Oncology Reports 36, no. 6 (2016): 3536-3544. https://doi.org/10.3892/or.2016.5162
Copy and paste a formatted citation
x
Spandidos Publications style
Li P, Ying J, Chang Q, Zhu W, Yang G, Xu T, Yi H, Pan R, Zhang E, Zeng X, Zeng X, et al: Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells. Oncol Rep 36: 3536-3544, 2016.
APA
Li, P., Ying, J., Chang, Q., Zhu, W., Yang, G., Xu, T. ... Li, S. (2016). Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells. Oncology Reports, 36, 3536-3544. https://doi.org/10.3892/or.2016.5162
MLA
Li, P., Ying, J., Chang, Q., Zhu, W., Yang, G., Xu, T., Yi, H., Pan, R., Zhang, E., Zeng, X., Yan, C., Bao, Q., Li, S."Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells". Oncology Reports 36.6 (2016): 3536-3544.
Chicago
Li, P., Ying, J., Chang, Q., Zhu, W., Yang, G., Xu, T., Yi, H., Pan, R., Zhang, E., Zeng, X., Yan, C., Bao, Q., Li, S."Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells". Oncology Reports 36, no. 6 (2016): 3536-3544. https://doi.org/10.3892/or.2016.5162
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