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Article

Bax-interacting factor-1 inhibits cell proliferation and promotes apoptosis in prostate cancer cells

  • Authors:
    • Lin Xu
    • Zhu Wang
    • Shan-Yang He
    • Su-Fen Zhang
    • Hong-Jiao Luo
    • Kai Zhou
    • Xiao-Fei Li
    • Shao-Peng Qiu
    • Kai-Yuan Cao
  • View Affiliations / Copyright

    Affiliations: Research Center for Clinical Laboratory Standard, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, P.R. China, Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, P.R. China, Department of Urology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, P.R. China
  • Pages: 3513-3521
    |
    Published online on: October 14, 2016
       https://doi.org/10.3892/or.2016.5172
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Abstract

Prostate cancer (PCa) is one of the most common malignant tumors and the second leading cause of cancer-related death among males. Bax-interacting factor-1 (Bif-1) is a member of Endophilin family, which binds to and activates the BAX protein in response to the apoptosis signaling pathway. Loss of Bif-1 may suppress the intrinsic pathway of apoptosis and promote tumorigenesis, but there is also converse evidence that Bif-1 could in part be responsible for the tumorigenesis and the role of Bif-1 in PCa development is not clear. In the present study, we aimed to understand the relationships between Bif-1 expression and PCa development. The mRNA and protein expression levels of Bif-1 in PCa cell lines, benign prostatic hyperplasia (BPH) (n=100) and PCa tissues (n=100, including low Gleason-scored PCa n=43 and high Gleason-scored PCa n=57) were detected and the effects of Bif-1 overexpression on the apoptosis, proliferation and migration in LNCaP cells were explored. Bif-1 mRNA levels of PCa cell lines were analyzed by real-time PCR and the protein levels were detected by western blotting. Bif-1 expression in BPH and PCa samples was detected by immunohistochemistry. To build Bif-1 overexpression PCa cells, Bif-1 gene was transfected into LNCaP cells by pcDNA3.1(+)‑Bif-1 vector. Cell apoptosis was detected by flow cytometric analysis, cell proliferation measured by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay and cell migration was analyzed by wound‑healing assay. The results proved that Bif-1 is downregulated in both PCa cell lines (P<0.01) and clinical samples (P<0.05), and Bif-1 expression is suppressed with the cancer progression (BPH vs. PCa P<0.01, and low Gleason-scored PCa vs. high Gleason-scored PCa P<0.05). Overexpression of Bif-1 could significantly inhibit cell proliferation (P<0.05) and enhancing PCa cell apoptosis (P<0.05), but it did not affect the migration ability (P>0.05). Our findings give strong evidence that Bif-1 is involved in PCa tumorigenesis and acts as a suppressor in PCa progression, and may have significance in understanding the process of PCa development.
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Copy and paste a formatted citation
Spandidos Publications style
Xu L, Wang Z, He S, Zhang S, Luo H, Zhou K, Li X, Qiu S and Cao K: Bax-interacting factor-1 inhibits cell proliferation and promotes apoptosis in prostate cancer cells. Oncol Rep 36: 3513-3521, 2016.
APA
Xu, L., Wang, Z., He, S., Zhang, S., Luo, H., Zhou, K. ... Cao, K. (2016). Bax-interacting factor-1 inhibits cell proliferation and promotes apoptosis in prostate cancer cells. Oncology Reports, 36, 3513-3521. https://doi.org/10.3892/or.2016.5172
MLA
Xu, L., Wang, Z., He, S., Zhang, S., Luo, H., Zhou, K., Li, X., Qiu, S., Cao, K."Bax-interacting factor-1 inhibits cell proliferation and promotes apoptosis in prostate cancer cells". Oncology Reports 36.6 (2016): 3513-3521.
Chicago
Xu, L., Wang, Z., He, S., Zhang, S., Luo, H., Zhou, K., Li, X., Qiu, S., Cao, K."Bax-interacting factor-1 inhibits cell proliferation and promotes apoptosis in prostate cancer cells". Oncology Reports 36, no. 6 (2016): 3513-3521. https://doi.org/10.3892/or.2016.5172
Copy and paste a formatted citation
x
Spandidos Publications style
Xu L, Wang Z, He S, Zhang S, Luo H, Zhou K, Li X, Qiu S and Cao K: Bax-interacting factor-1 inhibits cell proliferation and promotes apoptosis in prostate cancer cells. Oncol Rep 36: 3513-3521, 2016.
APA
Xu, L., Wang, Z., He, S., Zhang, S., Luo, H., Zhou, K. ... Cao, K. (2016). Bax-interacting factor-1 inhibits cell proliferation and promotes apoptosis in prostate cancer cells. Oncology Reports, 36, 3513-3521. https://doi.org/10.3892/or.2016.5172
MLA
Xu, L., Wang, Z., He, S., Zhang, S., Luo, H., Zhou, K., Li, X., Qiu, S., Cao, K."Bax-interacting factor-1 inhibits cell proliferation and promotes apoptosis in prostate cancer cells". Oncology Reports 36.6 (2016): 3513-3521.
Chicago
Xu, L., Wang, Z., He, S., Zhang, S., Luo, H., Zhou, K., Li, X., Qiu, S., Cao, K."Bax-interacting factor-1 inhibits cell proliferation and promotes apoptosis in prostate cancer cells". Oncology Reports 36, no. 6 (2016): 3513-3521. https://doi.org/10.3892/or.2016.5172
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