Effect of colorectal cancer on the number of normal stem cells circulating in peripheral blood

  • Authors:
    • Wojciech Marlicz
    • Katarzyna Sielatycka
    • Karol Serwin
    • Ewa Kubis
    • Marta Tkacz
    • Rafał Głuszko
    • Andrzej Białek
    • Teresa Starzyńska
    • Mariusz Z. Ratajczak
  • View Affiliations

  • Published online on: October 18, 2016     https://doi.org/10.3892/or.2016.5179
  • Pages: 3635-3642
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Abstract

Bone marrow (BM) residing stem cells are mobilized from their BM niches into peripheral blood (PB) in several pathological situations including tissue organ injury and systemic inflammation. We recently reported that the number of BM-derived stem cells (SCs) increases in patients with pancreatic and stomach cancer. Accordingly, we observed higher numbers of circulating very small embryonic/epiblast‑like stem cells (VSELs) and mesenchymal stem cells (MSCs) that were associated with the activation of pro-mobilizing complement cascade and an elevated level of sphingosine-1 phosphate (S1P) in PB plasma. We wondered if a similar correlation occurs in patients with colorectal cancer (CRC). A total of 46 patients were enrolled in this study: 17 with CRC, 18 with benign colonic adenomas (BCA) and 11 healthy individuals. By employing fluorescence-activated cell sorting (FACS) we evaluated the number of BM-derived SCs circulating in PB: i) CD34+/Lin-/CD45- and CD133-/Lin-/CD45- VSELs; ii) CD45-/CD105+/CD90+/CD29+ MSCs; iii) CD45-/CD34+/CD133+/KDR+ endothelial progenitor cells (EPCs); and iv) CD133+/Lin-/CD45+ or CD34+/Lin-/CD45+ cells enriched for hematopoietic stem/progenitor cells (HSPCs). In parallel, we measured in the PB parameters regulating the egress of SCs from BM into PB. In contrast to pancreatic and gastric cancer patients, CRC subjects presented neither an increase in the number of circulating SCs nor the activation of pro-mobilizing factors such as complement, coagulation and fibrinolytic cascade, circulating stromal derived factor 1 (SDF‑1), vascular endothelial growth factor (VEGF) and intestinal permeability marker (zonulin). In conclusion, mobilization of SCs in cancer patients depends on the type of malignancy and its ability to activate pro-mobilization cascades.
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December-2016
Volume 36 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Marlicz W, Sielatycka K, Serwin K, Kubis E, Tkacz M, Głuszko R, Białek A, Starzyńska T and Ratajczak MZ: Effect of colorectal cancer on the number of normal stem cells circulating in peripheral blood. Oncol Rep 36: 3635-3642, 2016
APA
Marlicz, W., Sielatycka, K., Serwin, K., Kubis, E., Tkacz, M., Głuszko, R. ... Ratajczak, M.Z. (2016). Effect of colorectal cancer on the number of normal stem cells circulating in peripheral blood. Oncology Reports, 36, 3635-3642. https://doi.org/10.3892/or.2016.5179
MLA
Marlicz, W., Sielatycka, K., Serwin, K., Kubis, E., Tkacz, M., Głuszko, R., Białek, A., Starzyńska, T., Ratajczak, M. Z."Effect of colorectal cancer on the number of normal stem cells circulating in peripheral blood". Oncology Reports 36.6 (2016): 3635-3642.
Chicago
Marlicz, W., Sielatycka, K., Serwin, K., Kubis, E., Tkacz, M., Głuszko, R., Białek, A., Starzyńska, T., Ratajczak, M. Z."Effect of colorectal cancer on the number of normal stem cells circulating in peripheral blood". Oncology Reports 36, no. 6 (2016): 3635-3642. https://doi.org/10.3892/or.2016.5179