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Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore

  • Authors:
    • Wen-Yu Gao
    • Dan Li
    • De-En Cai
    • Xiao-Yun Huang
    • Bi-Yun Zheng
    • Yue-Hong Huang
    • Zhi-Xin Chen
    • Xiao-Zhong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Fujian Medical University Union Hospital, Fujian 350001, P.R. China, Graduate School, Fujian Medical University, Fujian 350001, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 48-56
    |
    Published online on: November 7, 2016
       https://doi.org/10.3892/or.2016.5225
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Abstract

Chronic hepatitis B virus (HBV) infection is a leading cause of liver cirrhosis and cancer. Among the pathogenic factors of HBV, HBV X protein (HBx) is attracting increased attention. Although it is documented that HBx is a multifunctional regulator that modulates cell inflammation and apoptosis, the exact mechanism remains controversial. In the present study, we explored the effect of HBx on oxidative stress-induced apoptosis in normal liver cell line, HL-7702. Our results showed that the existence of HBx affected mitochon­drial biogenesis by modulating the opening of the mitochondrial permeability transition pore (MPTP). Notably, this phenomenon was associated with a pronounced translocation of Bax from the cytosol to the mitochon­dria during the period of exposure to oxidative stress with a release of cytochrome c and activation of cleaved caspase-3 and PARP. Moreover, MPTP blockage with cyclosporin A prevented the translocation of Bax, and inhibited oxidative stress-induced apoptotic killing in the HBx-expressing HL-7702 cells. Our findings suggest that HBx exhibits pro-apoptotic effects upon normal liver cells following exposure to oxidative stress by modulating the MPTP gateway.
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Copy and paste a formatted citation
Spandidos Publications style
Gao W, Li D, Cai D, Huang X, Zheng B, Huang Y, Chen Z and Wang X: Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore. Oncol Rep 37: 48-56, 2017.
APA
Gao, W., Li, D., Cai, D., Huang, X., Zheng, B., Huang, Y. ... Wang, X. (2017). Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore. Oncology Reports, 37, 48-56. https://doi.org/10.3892/or.2016.5225
MLA
Gao, W., Li, D., Cai, D., Huang, X., Zheng, B., Huang, Y., Chen, Z., Wang, X."Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore". Oncology Reports 37.1 (2017): 48-56.
Chicago
Gao, W., Li, D., Cai, D., Huang, X., Zheng, B., Huang, Y., Chen, Z., Wang, X."Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore". Oncology Reports 37, no. 1 (2017): 48-56. https://doi.org/10.3892/or.2016.5225
Copy and paste a formatted citation
x
Spandidos Publications style
Gao W, Li D, Cai D, Huang X, Zheng B, Huang Y, Chen Z and Wang X: Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore. Oncol Rep 37: 48-56, 2017.
APA
Gao, W., Li, D., Cai, D., Huang, X., Zheng, B., Huang, Y. ... Wang, X. (2017). Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore. Oncology Reports, 37, 48-56. https://doi.org/10.3892/or.2016.5225
MLA
Gao, W., Li, D., Cai, D., Huang, X., Zheng, B., Huang, Y., Chen, Z., Wang, X."Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore". Oncology Reports 37.1 (2017): 48-56.
Chicago
Gao, W., Li, D., Cai, D., Huang, X., Zheng, B., Huang, Y., Chen, Z., Wang, X."Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore". Oncology Reports 37, no. 1 (2017): 48-56. https://doi.org/10.3892/or.2016.5225
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