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Article

Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer

  • Authors:
    • Jiaying Zhao
    • You Lv
    • Yuankun Cai
    • Wangui Wei
    • Chenqing Yin
    • Xin Wang
    • Zong Hao
    • Chenxia Shen
    • Huipeng Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China, Life Science Research Institute, Chinese Academy of Sciences, Shanghai 200031, P.R. China
  • Pages: 492-500
    |
    Published online on: November 8, 2016
       https://doi.org/10.3892/or.2016.5229
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Abstract

The effect of hyperthermic carbon dioxide (CO2) pneumoperitoneum in combination with 5-fluorouracil (5-FU) on the proliferation and invasion of colon cancer was explored. Colon cancer cell line SW-480 was sealed into the urine collection bag to simulate pneumoperitoneum with 100% CO2 under a pressure of 12 mmHg. The cells were divided into group A, CO2 at 37˚C; group B, CO2 at 43˚C; group C, 5-FU; group D, CO2 at 37˚C+5-FU; group E, CO2 at 43˚C+5-FU; and control groups under normal culture conditions. The cell proliferation was assessed by CCK-8 test; the cell apoptosis was tested by FACS analysis; the cell invasion was examined by Transwell assay; the expression of HSP-70, caspase-3, HIF-1α and MMP-9 proteins and genes were detected by western blot analysis and RT-PCR. The SW-480 cells were injected into nude mouse cecum subserosal to establish a colon cancer model. We applied 43˚C CO2 pneumoperitoneum or 5-FU intraperitoneal chemotherapy to intervene, detected the transplantation tumor growth and metastasis. The cell proliferation was inhibited in groups B, C, D and E, apoptosis was induced in groups B, C, D and E, the Transwell cell number decreased in groups B, C, D and E, the transplantation tumor weight and metastasis rate were inhibited in groups B, C, D and E, but all not in group A. The most significant change was observed in group E. Hyperthermic CO2 pneumoperitoneum was able to reinforce the inhibition of 5-FU on proliferation and invasion of colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao J, Lv Y, Cai Y, Wei W, Yin C, Wang X, Hao Z, Shen C and Wang H: Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer. Oncol Rep 37: 492-500, 2017.
APA
Zhao, J., Lv, Y., Cai, Y., Wei, W., Yin, C., Wang, X. ... Wang, H. (2017). Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer. Oncology Reports, 37, 492-500. https://doi.org/10.3892/or.2016.5229
MLA
Zhao, J., Lv, Y., Cai, Y., Wei, W., Yin, C., Wang, X., Hao, Z., Shen, C., Wang, H."Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer". Oncology Reports 37.1 (2017): 492-500.
Chicago
Zhao, J., Lv, Y., Cai, Y., Wei, W., Yin, C., Wang, X., Hao, Z., Shen, C., Wang, H."Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer". Oncology Reports 37, no. 1 (2017): 492-500. https://doi.org/10.3892/or.2016.5229
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao J, Lv Y, Cai Y, Wei W, Yin C, Wang X, Hao Z, Shen C and Wang H: Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer. Oncol Rep 37: 492-500, 2017.
APA
Zhao, J., Lv, Y., Cai, Y., Wei, W., Yin, C., Wang, X. ... Wang, H. (2017). Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer. Oncology Reports, 37, 492-500. https://doi.org/10.3892/or.2016.5229
MLA
Zhao, J., Lv, Y., Cai, Y., Wei, W., Yin, C., Wang, X., Hao, Z., Shen, C., Wang, H."Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer". Oncology Reports 37.1 (2017): 492-500.
Chicago
Zhao, J., Lv, Y., Cai, Y., Wei, W., Yin, C., Wang, X., Hao, Z., Shen, C., Wang, H."Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer". Oncology Reports 37, no. 1 (2017): 492-500. https://doi.org/10.3892/or.2016.5229
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