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Article Open Access

Degradation of P-glycoprotein by pristimerin contributes to overcoming ABCB1-mediated chemotherapeutic drug resistance in vitro

  • Authors:
    • Yan-Yan Yan
    • Fang Wang
    • Xiao-Qin Zhao
    • Xiao-Kun Wang
    • Yi-Fan Chen
    • Hong Liu
    • Yong Xie
    • Li-Wu Fu
  • View Affiliations / Copyright

    Affiliations: Institute of Respiratory and Occupational Diseases, Collaborative Innovation Center for Cancer, Shanxi Datong University, Datong, Shanxi 037009, P.R. China, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Cancer Center, Sun Yat-Sen University, Guangzhou, Guangdong 510060, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 31-40
    |
    Published online on: November 8, 2016
       https://doi.org/10.3892/or.2016.5230
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Abstract

ABCB1 (P-glycoprotein, ABCB1/MDR1) is one of the major members of the ABC transporters linked to MDR in cancer cells. In this study, we observed that pristimerin, a natural triterpenoid, potently decreased P-gp in a dose-dependent manner in both drug-resistant KBv200 and stable transfected HEK293/ABCB1 cell lines. Moreover, pristimerin also inhibited cell proliferation and induced apoptosis in both cell lines. Intriguingly, reverse transcription-PCR, real-time PCR and protein turn-over assay revealed that the decrease of P-gp was independent of mRNA level but primarily owing to its protein stability. Furthermore, immunofluorescence study with anti-P-gp antibody showed that pristimerin disturbed the subcellular distribution of P-gp with decreased location in the plasma membrane. Taken together, these data suggest that subcellular distribution of P-gp and subsequent downregulation by pristimerin contribute to overcoming ABCB1-mediated chemotherapeutic drug resistance. Our findings suggested inducing the decrease of P-gp membrane protein could be a new promising alternative therapeutic strategy in ABCB1-mediated MDR.
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Copy and paste a formatted citation
Spandidos Publications style
Yan Y, Wang F, Zhao X, Wang X, Chen Y, Liu H, Xie Y and Fu L: Degradation of P-glycoprotein by pristimerin contributes to overcoming ABCB1-mediated chemotherapeutic drug resistance in vitro. Oncol Rep 37: 31-40, 2017.
APA
Yan, Y., Wang, F., Zhao, X., Wang, X., Chen, Y., Liu, H. ... Fu, L. (2017). Degradation of P-glycoprotein by pristimerin contributes to overcoming ABCB1-mediated chemotherapeutic drug resistance in vitro. Oncology Reports, 37, 31-40. https://doi.org/10.3892/or.2016.5230
MLA
Yan, Y., Wang, F., Zhao, X., Wang, X., Chen, Y., Liu, H., Xie, Y., Fu, L."Degradation of P-glycoprotein by pristimerin contributes to overcoming ABCB1-mediated chemotherapeutic drug resistance in vitro". Oncology Reports 37.1 (2017): 31-40.
Chicago
Yan, Y., Wang, F., Zhao, X., Wang, X., Chen, Y., Liu, H., Xie, Y., Fu, L."Degradation of P-glycoprotein by pristimerin contributes to overcoming ABCB1-mediated chemotherapeutic drug resistance in vitro". Oncology Reports 37, no. 1 (2017): 31-40. https://doi.org/10.3892/or.2016.5230
Copy and paste a formatted citation
x
Spandidos Publications style
Yan Y, Wang F, Zhao X, Wang X, Chen Y, Liu H, Xie Y and Fu L: Degradation of P-glycoprotein by pristimerin contributes to overcoming ABCB1-mediated chemotherapeutic drug resistance in vitro. Oncol Rep 37: 31-40, 2017.
APA
Yan, Y., Wang, F., Zhao, X., Wang, X., Chen, Y., Liu, H. ... Fu, L. (2017). Degradation of P-glycoprotein by pristimerin contributes to overcoming ABCB1-mediated chemotherapeutic drug resistance in vitro. Oncology Reports, 37, 31-40. https://doi.org/10.3892/or.2016.5230
MLA
Yan, Y., Wang, F., Zhao, X., Wang, X., Chen, Y., Liu, H., Xie, Y., Fu, L."Degradation of P-glycoprotein by pristimerin contributes to overcoming ABCB1-mediated chemotherapeutic drug resistance in vitro". Oncology Reports 37.1 (2017): 31-40.
Chicago
Yan, Y., Wang, F., Zhao, X., Wang, X., Chen, Y., Liu, H., Xie, Y., Fu, L."Degradation of P-glycoprotein by pristimerin contributes to overcoming ABCB1-mediated chemotherapeutic drug resistance in vitro". Oncology Reports 37, no. 1 (2017): 31-40. https://doi.org/10.3892/or.2016.5230
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