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Article

Expression of angiogenic growth factors VEGF, bFGF and ANG1 in colon cancer after bevacizumab treatment in vitro: A potential self-regulating mechanism

  • Authors:
    • Minjian Zhao
    • Zhaoshi Yu
    • Zhihong Li
    • Jun Tang
    • Xu Lai
    • Liming Liu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Central Hospital of Ezhou, Hubei 436000, P.R. China
  • Pages: 601-607
    |
    Published online on: November 8, 2016
       https://doi.org/10.3892/or.2016.5231
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Abstract

The present study aimed to examine changes in the expression of angiogenic growth factors in vascular endothelial cells isolated from colon cancer after bevacizumab treatment in vitro, and to explore a potential mechanism of their self-regulation as a possible mechanism for antiangiogenic therapy failure in clinics. Vascular endothelial cells were isolated from tumors of colon cancer patients and transfected with recombinant adeno-associated virus type 2-vascular endothelial growth factor (VEGF) or pGIPZ-VEGF RNA interference in order to upregulate or downregulate VEGF expression. Changes in VEGF expression and its correlation with the expression of angiogenesis-related factors, including basic fibroblast growth factor (bFGF) and angiopoietin 1 (ANG1), after treatment with bevacizumab in vitro, were investigated. The results showed that in cells with VEGF overexpression, bFGF and ANG1 were downregulated, whereas in cells in which VEGF was knocked down, upregulation of bFGF and ANG1 was detected. In cells treated with bevacizumab, a significant upregulation of VEGF and downregulation of bFGF and ANG1 were observed. Our data indicate that after bevacizumab treatment, a potential self-regulating mechanism of angiogenic growth factors in colon cancer-derived endothelial cells is activated, which may explain why current antiangiogenic therapy with bevacizumab has limited effects in prolonging the survival of colon cancer patients.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao M, Yu , Li Z, Tang J, Lai X and Liu L: Expression of angiogenic growth factors VEGF, bFGF and ANG1 in colon cancer after bevacizumab treatment in vitro: A potential self-regulating mechanism. Oncol Rep 37: 601-607, 2017.
APA
Zhao, M., Yu, ., Li, Z., Tang, J., Lai, X., & Liu, L. (2017). Expression of angiogenic growth factors VEGF, bFGF and ANG1 in colon cancer after bevacizumab treatment in vitro: A potential self-regulating mechanism. Oncology Reports, 37, 601-607. https://doi.org/10.3892/or.2016.5231
MLA
Zhao, M., Yu, ., Li, Z., Tang, J., Lai, X., Liu, L."Expression of angiogenic growth factors VEGF, bFGF and ANG1 in colon cancer after bevacizumab treatment in vitro: A potential self-regulating mechanism". Oncology Reports 37.1 (2017): 601-607.
Chicago
Zhao, M., Yu, ., Li, Z., Tang, J., Lai, X., Liu, L."Expression of angiogenic growth factors VEGF, bFGF and ANG1 in colon cancer after bevacizumab treatment in vitro: A potential self-regulating mechanism". Oncology Reports 37, no. 1 (2017): 601-607. https://doi.org/10.3892/or.2016.5231
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao M, Yu , Li Z, Tang J, Lai X and Liu L: Expression of angiogenic growth factors VEGF, bFGF and ANG1 in colon cancer after bevacizumab treatment in vitro: A potential self-regulating mechanism. Oncol Rep 37: 601-607, 2017.
APA
Zhao, M., Yu, ., Li, Z., Tang, J., Lai, X., & Liu, L. (2017). Expression of angiogenic growth factors VEGF, bFGF and ANG1 in colon cancer after bevacizumab treatment in vitro: A potential self-regulating mechanism. Oncology Reports, 37, 601-607. https://doi.org/10.3892/or.2016.5231
MLA
Zhao, M., Yu, ., Li, Z., Tang, J., Lai, X., Liu, L."Expression of angiogenic growth factors VEGF, bFGF and ANG1 in colon cancer after bevacizumab treatment in vitro: A potential self-regulating mechanism". Oncology Reports 37.1 (2017): 601-607.
Chicago
Zhao, M., Yu, ., Li, Z., Tang, J., Lai, X., Liu, L."Expression of angiogenic growth factors VEGF, bFGF and ANG1 in colon cancer after bevacizumab treatment in vitro: A potential self-regulating mechanism". Oncology Reports 37, no. 1 (2017): 601-607. https://doi.org/10.3892/or.2016.5231
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