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Article

Restoration of the tumor-suppressor function to mutant p53 by Ganoderma lucidum polysaccharides in colorectal cancer cells

  • Authors:
    • Dan Jiang
    • Lingyao Wang
    • Tong Zhao
    • Zhaoyu Zhang
    • Renxia Zhang
    • Jingji Jin
    • Yong Cai
    • Fei Wang
  • View Affiliations / Copyright

    Affiliations: School of Life Sciences, Jilin University, Changchun, Jilin 130012, P.R. China
  • Pages: 594-600
    |
    Published online on: November 15, 2016
       https://doi.org/10.3892/or.2016.5246
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Abstract

Ganoderma lucidum polysaccharides (GLPs), isolated from spores, mycelia and fruiting bodies of Ganoderma lucidum, have been suggested to possess anticancer activities in a large number of basic studies. A recent survey revealed that GLP-induced inhibition of cancer cell growth was dependent on the existence of functional p53. However, the actual role of p53-mediated tumor-suppressing pathways in facilitating the anticancer effect of GLPs is still unclear. In the present study, we investigated the interaction between GLPs and mutant p53 that exists in more than half of the known types of cancers. Our results showed that GLPs reactivated mutant p53 in colorectal cancer HT29 (p53R273H) and SW480 (p53R273H&P309S) cells while applied alone or together with 5-fluorouracil (5-FU). This reactivation further induced cell growth inhibition and apoptosis. In addition, western blot assay and in vitro cell-free apoptosis assay suggested that the activation of mutant p53 was effective in both a transcriptional-dependent and -independent pathway. Altogether, our data demonstrated for the first time that GLPs show prominent anticancer activities by reactivating several types of mutant p53. Therefore, targeting mutant p53 by GLPs alongside other chemotherapeutics may be considered as a novel treatment strategy for cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang D, Wang L, Zhao T, Zhang Z, Zhang R, Jin J, Cai Y and Wang F: Restoration of the tumor-suppressor function to mutant p53 by Ganoderma lucidum polysaccharides in colorectal cancer cells. Oncol Rep 37: 594-600, 2017.
APA
Jiang, D., Wang, L., Zhao, T., Zhang, Z., Zhang, R., Jin, J. ... Wang, F. (2017). Restoration of the tumor-suppressor function to mutant p53 by Ganoderma lucidum polysaccharides in colorectal cancer cells. Oncology Reports, 37, 594-600. https://doi.org/10.3892/or.2016.5246
MLA
Jiang, D., Wang, L., Zhao, T., Zhang, Z., Zhang, R., Jin, J., Cai, Y., Wang, F."Restoration of the tumor-suppressor function to mutant p53 by Ganoderma lucidum polysaccharides in colorectal cancer cells". Oncology Reports 37.1 (2017): 594-600.
Chicago
Jiang, D., Wang, L., Zhao, T., Zhang, Z., Zhang, R., Jin, J., Cai, Y., Wang, F."Restoration of the tumor-suppressor function to mutant p53 by Ganoderma lucidum polysaccharides in colorectal cancer cells". Oncology Reports 37, no. 1 (2017): 594-600. https://doi.org/10.3892/or.2016.5246
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang D, Wang L, Zhao T, Zhang Z, Zhang R, Jin J, Cai Y and Wang F: Restoration of the tumor-suppressor function to mutant p53 by Ganoderma lucidum polysaccharides in colorectal cancer cells. Oncol Rep 37: 594-600, 2017.
APA
Jiang, D., Wang, L., Zhao, T., Zhang, Z., Zhang, R., Jin, J. ... Wang, F. (2017). Restoration of the tumor-suppressor function to mutant p53 by Ganoderma lucidum polysaccharides in colorectal cancer cells. Oncology Reports, 37, 594-600. https://doi.org/10.3892/or.2016.5246
MLA
Jiang, D., Wang, L., Zhao, T., Zhang, Z., Zhang, R., Jin, J., Cai, Y., Wang, F."Restoration of the tumor-suppressor function to mutant p53 by Ganoderma lucidum polysaccharides in colorectal cancer cells". Oncology Reports 37.1 (2017): 594-600.
Chicago
Jiang, D., Wang, L., Zhao, T., Zhang, Z., Zhang, R., Jin, J., Cai, Y., Wang, F."Restoration of the tumor-suppressor function to mutant p53 by Ganoderma lucidum polysaccharides in colorectal cancer cells". Oncology Reports 37, no. 1 (2017): 594-600. https://doi.org/10.3892/or.2016.5246
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