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Article

miR-148a suppresses human renal cell carcinoma malignancy by targeting AKT2

Retraction in: /10.3892/or.2022.8454
  • Authors:
    • Huiyan Cao
    • Zhiming Liu
    • Rong Wang
    • Xiaodong Zhang
    • Wenfa Yi
    • Guanyuan Nie
    • Yong Yu
    • Guolu Wang
    • Mingting Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Urological Surgery, People's Hospital of Qinghai, Xining, Qinghai 810007, P.R. China, Department of Medical Oncology, The Affiliated Hospital of Qinghai University, Xining, Qinghai 810001, P.R. China
  • Pages: 147-154
    |
    Published online on: November 18, 2016
       https://doi.org/10.3892/or.2016.5257
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Abstract

MicroRNA-148a (miR-148a) has been reported to be deregulated in different tumor types, whereas the biological function of miR-148a in renal cell carcinoma (RCC) largely remains unexplored. In the present study we investigated the clinical significance, biological effects, and the underlying molecular mechanisms of miR-148 in RCC. Here, we showed that miR-148a was significantly downregulated in RCC tissues and cell lines. Low expression of miR-148a in RCC tissues was associated with large tumor size, advanced TNM stage, and lymph node metastasis. Functional assays revealed that overexpression of miR-148a significantly inhibited RCC cell proliferation, colony formation, migration and invasion in vitro and suppressed RCC xenograft tumor growth in vivo. In addition, using quantitative RT-PCR (qRT-PCR), western blot analysis and luciferase reporter assays, AKT2 was confirmed to be a direct target of miR-148a. AKT2 expression was upregulated, and was negatively correlated with miR-148a expression in RCC tissues (r=-0.641, P<0.001). Silencing of AKT2 phenotypically copied miR-148a-induced phenotypes, whereas re-expression of AKT2 reversed the suppressive effects of miR-148a in RCC cells. Further mechanistic investigations showed that miR-148a exerted its antitumor activity via inhibition of the AKT pathway in vitro and in vivo. Taken together, these findings suggest that miR-148a functions as tumor suppressor in RCC by targeting AKT2.
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1 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

2 

DeSantis CE, Lin CC, Mariotto AB, Siegel RL, Stein KD, Kramer JL, Alteri R, Robbins AS and Jemal A: Cancer treatment and survivorship statistics, 2014. CA Cancer J Clin. 64:252–271. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Capitanio U and Montorsi F: Renal cancer. Lancet. 387:894–906. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Chow WH, Dong LM and Devesa SS: Epidemiology and risk factors for kidney cancer. Nat Rev Urol. 7:245–257. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Pantuck AJ, Zisman A and Belldegrun AS: The changing natural history of renal cell carcinoma. J Urol. 166:1611–1623. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Ying SY, Chang DC, Miller JD and Lin SL: The microRNA: Overview of the RNA gene that modulates gene functions. Methods Mol Biol. 342:1–18. 2006.PubMed/NCBI

7 

Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, Ebert BL, Mak RH, Ferrando AA, et al: MicroRNA expression profiles classify human cancers. Nature. 435:834–838. 2005. View Article : Google Scholar : PubMed/NCBI

8 

McManus MT: MicroRNAs and cancer. Semin Cancer Biol. 13:253–258. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Calin GA and Croce CM: MicroRNA-cancer connection: The beginning of a new tale. Cancer Res. 66:7390–7394. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Gu L, Li H, Chen L, Ma X, Gao Y, Li X, Zhang Y, Fan Y and Zhang X: MicroRNAs as prognostic molecular signatures in renal cell carcinoma: A systematic review and meta-analysis. Oncotarget. 6:32545–32560. 2015.PubMed/NCBI

11 

Li JY, Yong TY, Michael MZ and Gleadle JM: Review: The role of microRNAs in kidney disease. Nephrology (Carlton). 15:599–608. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Xu X, Zhang Y, Jasper J, Lykken E, Alexander PB, Markowitz GJ, McDonnell DP, Li QJ and Wang XF: MiR-148a functions to suppress metastasis and serves as a prognostic indicator in triple-negative breast cancer. Oncotarget. 7:20381–20394. 2016.PubMed/NCBI

13 

Pan L, Huang S, He R, Rong M, Dang Y and Chen G: Decreased expression and clinical significance of miR-148a in hepatocellular carcinoma tissues. Eur J Med Res. 19:682014. View Article : Google Scholar : PubMed/NCBI

14 

Ma W, Zhang X, Chai J, Chen P, Ren P and Gong M: Circulating miR-148a is a significant diagnostic and prognostic biomarker for patients with osteosarcoma. Tumour Biol. 35:12467–12472. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Xia J, Guo X, Yan J and Deng K: The role of miR-148a in gastric cancer. J Cancer Res Clin Oncol. 140:1451–1456. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Hibino Y, Sakamoto N, Naito Y, Goto K, Oo HZ, Sentani K, Hinoi T, Ohdan H, Oue N and Yasui W: Significance of miR-148a in colorectal neoplasia: Downregulation of miR-148a contributes to the carcinogenesis and cell invasion of colorectal cancer. Pathobiology. 82:233–241. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Yogi K, Sridhar E, Goel N, Jalali R, Goel A, Moiyadi A, Thorat R, Panwalkar P, Khire A, Dasgupta A, et al: MiR-148a, a microRNA upregulated in the WNT subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting neuropilin 1. Oncoscience. 2:334–348. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Lombard AP, Mooso BA, Libertini SJ, Lim RM, Nakagawa RM, Vidallo KD, Costanzo NC, Ghosh PM and Mudryj M: miR-148a dependent apoptosis of bladder cancer cells is mediated in part by the epigenetic modifier DNMT1. Mol Carcinog. 55:757–767. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Joshi P, Jeon YJ, Laganà A, Middleton J, Secchiero P, Garofalo M and Croce CM: MicroRNA-148a reduces tumorigenesis and increases TRAIL-induced apoptosis in NSCLC. Proc Natl Acad Sci USA. 112:8650–8655. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Xu Q, Jiang Y, Yin Y, Li Q, He J, Jing Y, Qi YT, Xu Q, Li W, Lu B, et al: A regulatory circuit of miR-148a/152 and DNMT1 in modulating cell transformation and tumor angiogenesis through IGF-IR and IRS1. J Mol Cell Biol. 5:3–13. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Li M, Li H, Liu X, Xu D and Wang F: MicroRNA-29b regulates TGF-β1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells by targeting AKT2. Exp Cell Res. 345:115–124. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Kim J, Zhang Y, Skalski M, Hayes J, Kefas B, Schiff D, Purow B, Parsons S, Lawler S and Abounader R: microRNA-148a is a prognostic oncomiR that targets MIG6 and BIM to regulate EGFR and apoptosis in glioblastoma. Cancer Res. 74:1541–1553. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Pereira L, Horta S, Mateus R and Videira MA: Implications of Akt2/Twist crosstalk on breast cancer metastatic outcome. Drug Discov Today. 20:1152–1158. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Agarwal E, Brattain MG and Chowdhury S: Cell survival and metastasis regulation by Akt signaling in colorectal cancer. Cell Signal. 25:1711–1719. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Cheng GZ, Zhang W and Wang LH: Regulation of cancer cell survival, migration, and invasion by Twist: AKT2 comes to interplay. Cancer Res. 68:957–960. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Toschi A, Lee E, Gadir N, Ohh M and Foster DA: Differential dependence of hypoxia-inducible factors 1 alpha and 2 alpha on mTORC1 and mTORC2. J Biol Chem. 283:34495–34499. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Jiang CF, Li DM, Shi ZM, Wang L, Liu MM, Ge X, Liu X, Qian YC, Wen YY, Zhen LL, et al: Estrogen regulates miRNA expression: Implication of estrogen receptor and miR-124/AKT2 in tumor growth and angiogenesis. Oncotarget. 7:36940–36955. 2016.PubMed/NCBI

28 

Sheng L, He P, Yang X, Zhou M and Feng Q: miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2. Cell Death Dis. 6:e18082015. View Article : Google Scholar : PubMed/NCBI

29 

Wu L, Chen J, Ding C, Wei S, Zhu Y, Yang W, Zhang X, Wei X and Han D: MicroRNA-137 contributes to dampened tumorigenesis in human gastric cancer by targeting AKT2. PLoS One. 10:e01301242015. View Article : Google Scholar : PubMed/NCBI

30 

Bai Y, Li J, Li J, Liu Y and Zhang B: MiR-615 inhibited cell proliferation and cell cycle of human breast cancer cells by suppressing of AKT2 expression. Int J Clin Exp Med. 8:3801–3808. 2015.PubMed/NCBI

31 

Chen H, Zhou L, Wu X, Li R, Wen J, Sha J and Wen X: The PI3K/AKT pathway in the pathogenesis of prostate cancer. Front Biosci (Landmark Ed). 21:1084–1091. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Yang SX, Polley E and Lipkowitz S: New insights on PI3K/AKT pathway alterations and clinical outcomes in breast cancer. Cancer Treat Rev. 45:87–96. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Robbins HL and Hague A: The PI3K/Akt pathway in tumors of endocrine tissues. Front Endocrinol (Lausanne). 6:1882016.PubMed/NCBI

34 

Guo H, German P, Bai S, Barnes S, Guo W, Qi X, Lou H, Liang J, Jonasch E, Mills GB, et al: The PI3K/AKT pathway and renal cell carcinoma. J Genet Genomics. 42:343–353. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Lee JJ, Loh K and Yap YS: PI3K/Akt/mTOR inhibitors in breast cancer. Cancer Biol Med. 12:342–354. 2015.PubMed/NCBI

36 

Hudes GR: Targeting mTOR in renal cell carcinoma. Cancer 115 (Suppl 10). 2313–2320. 2009. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Cao H, Liu Z, Wang R, Zhang X, Yi W, Nie G, Yu Y, Wang G and Zhu M: miR-148a suppresses human renal cell carcinoma malignancy by targeting AKT2 Retraction in /10.3892/or.2022.8454. Oncol Rep 37: 147-154, 2017.
APA
Cao, H., Liu, Z., Wang, R., Zhang, X., Yi, W., Nie, G. ... Zhu, M. (2017). miR-148a suppresses human renal cell carcinoma malignancy by targeting AKT2 Retraction in /10.3892/or.2022.8454. Oncology Reports, 37, 147-154. https://doi.org/10.3892/or.2016.5257
MLA
Cao, H., Liu, Z., Wang, R., Zhang, X., Yi, W., Nie, G., Yu, Y., Wang, G., Zhu, M."miR-148a suppresses human renal cell carcinoma malignancy by targeting AKT2 Retraction in /10.3892/or.2022.8454". Oncology Reports 37.1 (2017): 147-154.
Chicago
Cao, H., Liu, Z., Wang, R., Zhang, X., Yi, W., Nie, G., Yu, Y., Wang, G., Zhu, M."miR-148a suppresses human renal cell carcinoma malignancy by targeting AKT2 Retraction in /10.3892/or.2022.8454". Oncology Reports 37, no. 1 (2017): 147-154. https://doi.org/10.3892/or.2016.5257
Copy and paste a formatted citation
x
Spandidos Publications style
Cao H, Liu Z, Wang R, Zhang X, Yi W, Nie G, Yu Y, Wang G and Zhu M: miR-148a suppresses human renal cell carcinoma malignancy by targeting AKT2 Retraction in /10.3892/or.2022.8454. Oncol Rep 37: 147-154, 2017.
APA
Cao, H., Liu, Z., Wang, R., Zhang, X., Yi, W., Nie, G. ... Zhu, M. (2017). miR-148a suppresses human renal cell carcinoma malignancy by targeting AKT2 Retraction in /10.3892/or.2022.8454. Oncology Reports, 37, 147-154. https://doi.org/10.3892/or.2016.5257
MLA
Cao, H., Liu, Z., Wang, R., Zhang, X., Yi, W., Nie, G., Yu, Y., Wang, G., Zhu, M."miR-148a suppresses human renal cell carcinoma malignancy by targeting AKT2 Retraction in /10.3892/or.2022.8454". Oncology Reports 37.1 (2017): 147-154.
Chicago
Cao, H., Liu, Z., Wang, R., Zhang, X., Yi, W., Nie, G., Yu, Y., Wang, G., Zhu, M."miR-148a suppresses human renal cell carcinoma malignancy by targeting AKT2 Retraction in /10.3892/or.2022.8454". Oncology Reports 37, no. 1 (2017): 147-154. https://doi.org/10.3892/or.2016.5257
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