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miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer

  • Authors:
    • Qiang Fu
    • Jing Cheng
    • Jindai Zhang
    • Yonglei Zhang
    • Xiaobing Chen
    • Suxia Luo
    • Jianguo Xie

  • View Affiliations

  • Published online on: November 18, 2016     https://doi.org/10.3892/or.2016.5259
  • Pages: 123-130
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Abstract

Chemoresistance is a major obstacle to cancer therapy including that of colon cancer (CC). Although the dysregulation of many miRNAs has been implicated in 5-fluorouracil (5-FU) resistance in CC cells, the specific role of miR-20b in chemoresistance has not been documented. In the present study, we first determined the expression of miR-20b by RT-PCR and the levels of a disintegrin and metalloprotease 9 (ADAM9) and epidermal growth factor receptor (EGFR) by western blotting in CC and adjacent non-cancerous tissues from 5-FU-sensitive or -resistant CC patients. Subsequently, 5-FU-sensitive (HCT116) and -resistant (HCT116-R) cells were obtained, and the levels of miR-20b, ADAM9 and EGFR were detected. Meanwhile, the 5-FU resistance of the cells was examined by assessing cell viability (by MTT assay) and apoptosis (by flow cytometry). After transfection of miR-20b into HCT116-R cells, drug resistance was reexamined. We then confirmed the relationship between miR-20b and ADAM9 by luciferase reporter assay. Finally, 5-FU resistance in HCT116 and HCT116-R cells was compared after transfection with miR-20b. Our results showed that miR-20b was expressed at lower levels in the 5-FU-resistant tissues and cells than in the 5-FU-sensitive tissues and cells. The opposite was the case for expression of ADAM9 and EGFR. In addition, we demonstrated that ADAM9 is a direct target of miR-20b and that miR-20b decreased the 5-FU resistance of HCT116-R cells. Our findings suggest that miR-20b reduces 5-FU resistance to induce apoptosis in vitro by suppressing ADAM9/EGFR in CC cells.
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January-2017
Volume 37 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Impact Factor: 4.2
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  • CiteScore: 8.4
  • CiteScore Rank: Q1: #68/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.786
  • SCImago Journal Rank (SJR): 0.812
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Spandidos Publications style
Fu Q, Cheng J, Zhang J, Zhang Y, Chen X, Luo S and Xie J: miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer. Oncol Rep 37: 123-130, 2017
APA
Fu, Q., Cheng, J., Zhang, J., Zhang, Y., Chen, X., Luo, S., & Xie, J. (2017). miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer. Oncology Reports, 37, 123-130. https://doi.org/10.3892/or.2016.5259
MLA
Fu, Q., Cheng, J., Zhang, J., Zhang, Y., Chen, X., Luo, S., Xie, J."miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer". Oncology Reports 37.1 (2017): 123-130.
Chicago
Fu, Q., Cheng, J., Zhang, J., Zhang, Y., Chen, X., Luo, S., Xie, J."miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer". Oncology Reports 37, no. 1 (2017): 123-130. https://doi.org/10.3892/or.2016.5259