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Article

miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer

  • Authors:
    • Qiang Fu
    • Jing Cheng
    • Jindai Zhang
    • Yonglei Zhang
    • Xiaobing Chen
    • Suxia Luo
    • Jianguo Xie
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery Center, Henan Cancer Hospital, Zhengzhou, Henan 450008, P.R. China, Department of Medical Oncology, Zhengzhou Central Hospital, Zhengzhou, Henan 450007, P.R. China, Department of Digestion and Medical Oncology, Henan Cancer Hospital, Zhengzhou, Henan 450008, P.R. China
  • Pages: 123-130
    |
    Published online on: November 18, 2016
       https://doi.org/10.3892/or.2016.5259
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Abstract

Chemoresistance is a major obstacle to cancer therapy including that of colon cancer (CC). Although the dysregulation of many miRNAs has been implicated in 5-fluorouracil (5-FU) resistance in CC cells, the specific role of miR-20b in chemoresistance has not been documented. In the present study, we first determined the expression of miR-20b by RT-PCR and the levels of a disintegrin and metalloprotease 9 (ADAM9) and epidermal growth factor receptor (EGFR) by western blotting in CC and adjacent non-cancerous tissues from 5-FU-sensitive or -resistant CC patients. Subsequently, 5-FU-sensitive (HCT116) and -resistant (HCT116-R) cells were obtained, and the levels of miR-20b, ADAM9 and EGFR were detected. Meanwhile, the 5-FU resistance of the cells was examined by assessing cell viability (by MTT assay) and apoptosis (by flow cytometry). After transfection of miR-20b into HCT116-R cells, drug resistance was reexamined. We then confirmed the relationship between miR-20b and ADAM9 by luciferase reporter assay. Finally, 5-FU resistance in HCT116 and HCT116-R cells was compared after transfection with miR-20b. Our results showed that miR-20b was expressed at lower levels in the 5-FU-resistant tissues and cells than in the 5-FU-sensitive tissues and cells. The opposite was the case for expression of ADAM9 and EGFR. In addition, we demonstrated that ADAM9 is a direct target of miR-20b and that miR-20b decreased the 5-FU resistance of HCT116-R cells. Our findings suggest that miR-20b reduces 5-FU resistance to induce apoptosis in vitro by suppressing ADAM9/EGFR in CC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Fu Q, Cheng J, Zhang J, Zhang Y, Chen X, Luo S and Xie J: miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer. Oncol Rep 37: 123-130, 2017.
APA
Fu, Q., Cheng, J., Zhang, J., Zhang, Y., Chen, X., Luo, S., & Xie, J. (2017). miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer. Oncology Reports, 37, 123-130. https://doi.org/10.3892/or.2016.5259
MLA
Fu, Q., Cheng, J., Zhang, J., Zhang, Y., Chen, X., Luo, S., Xie, J."miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer". Oncology Reports 37.1 (2017): 123-130.
Chicago
Fu, Q., Cheng, J., Zhang, J., Zhang, Y., Chen, X., Luo, S., Xie, J."miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer". Oncology Reports 37, no. 1 (2017): 123-130. https://doi.org/10.3892/or.2016.5259
Copy and paste a formatted citation
x
Spandidos Publications style
Fu Q, Cheng J, Zhang J, Zhang Y, Chen X, Luo S and Xie J: miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer. Oncol Rep 37: 123-130, 2017.
APA
Fu, Q., Cheng, J., Zhang, J., Zhang, Y., Chen, X., Luo, S., & Xie, J. (2017). miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer. Oncology Reports, 37, 123-130. https://doi.org/10.3892/or.2016.5259
MLA
Fu, Q., Cheng, J., Zhang, J., Zhang, Y., Chen, X., Luo, S., Xie, J."miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer". Oncology Reports 37.1 (2017): 123-130.
Chicago
Fu, Q., Cheng, J., Zhang, J., Zhang, Y., Chen, X., Luo, S., Xie, J."miR-20b reduces 5-FU resistance by suppressing the ADAM9/EGFR signaling pathway in colon cancer". Oncology Reports 37, no. 1 (2017): 123-130. https://doi.org/10.3892/or.2016.5259
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