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Article

Adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system promotes the cell apoptosis of CD133+ ovarian cancer stem cells

  • Authors:
    • Qifang Long
    • Ru Yang
    • Weixian Lu
    • Weipei Zhu
    • Jundong Zhou
    • Cui Zheng
    • Dongmei Zhou
    • Ling Yu
    • Jinchang Wu
  • View Affiliations / Copyright

    Affiliations: Department of Radio-Oncology, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215001, P.R. China, Department of Gynecology and Obstetrics, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215001, P.R. China, Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China
  • Pages: 155-162
    |
    Published online on: November 21, 2016
       https://doi.org/10.3892/or.2016.5263
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Abstract

Cancer stem cells are a small subset of cancer cells that contribute to cancer progression, metastasis, chemoresistance and recurrence. CD133-positive (CD133+) ovarian cancer cells have been identified as ovarian cancer stem cells. Adenovirus-mediated gene therapy is an innovative therapeutic method for cancer treatment. In the present study, we aimed to develop a new gene therapy to specifically eliminate CD133+ ovarian cancer stem cells by targeting CD133. We used the Cre/LoxP system to augment the selective expression of the truncated Bid (tBid) gene as suicide gene therapy in CD133+ ovarian cancer stem cells. The adenovirus (Ad)-CD133-Cre expressing Cre recombinase under the control of the CD133 promoter and Ad-CMV-LoxP-Neo-LoxP-tBid expressing tBid under the control of the CMV promoter were successfully constructed using the Cre/LoxP switching system. The co-infection of Ad-CMV-LoxP-Neo-LoxP-tBid and Ad-CD133-Cre selectively induced tBid overexpression, which inhibited cell growth and triggered the cell apoptosis of CD133+ ovarian cancer stem cells. The Cre/LoxP system-mediated tBid overexpression activated the pro-apoptotic signaling pathway and augmented the cytotoxic effect of cisplatin in CD133+ ovarian cancer stem cells. Furthermore, in xenograft experiments, co-infection with the two recombinant adenoviruses markedly suppressed tumor growth in vivo and promoted cell apoptosis in tumor tissues. Taken together, the present study provides evidence that the adenovirus-mediated tBid overexpression induced by the Cre/LoxP system can effectively eliminate CD133+ ovarian cancer stem cells, representing a novel therapeutic strategy for the treatment of ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Long Q, Yang R, Lu W, Zhu W, Zhou J, Zheng C, Zhou D, Yu L and Wu J: Adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system promotes the cell apoptosis of CD133+ ovarian cancer stem cells. Oncol Rep 37: 155-162, 2017.
APA
Long, Q., Yang, R., Lu, W., Zhu, W., Zhou, J., Zheng, C. ... Wu, J. (2017). Adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system promotes the cell apoptosis of CD133+ ovarian cancer stem cells. Oncology Reports, 37, 155-162. https://doi.org/10.3892/or.2016.5263
MLA
Long, Q., Yang, R., Lu, W., Zhu, W., Zhou, J., Zheng, C., Zhou, D., Yu, L., Wu, J."Adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system promotes the cell apoptosis of CD133+ ovarian cancer stem cells". Oncology Reports 37.1 (2017): 155-162.
Chicago
Long, Q., Yang, R., Lu, W., Zhu, W., Zhou, J., Zheng, C., Zhou, D., Yu, L., Wu, J."Adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system promotes the cell apoptosis of CD133+ ovarian cancer stem cells". Oncology Reports 37, no. 1 (2017): 155-162. https://doi.org/10.3892/or.2016.5263
Copy and paste a formatted citation
x
Spandidos Publications style
Long Q, Yang R, Lu W, Zhu W, Zhou J, Zheng C, Zhou D, Yu L and Wu J: Adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system promotes the cell apoptosis of CD133+ ovarian cancer stem cells. Oncol Rep 37: 155-162, 2017.
APA
Long, Q., Yang, R., Lu, W., Zhu, W., Zhou, J., Zheng, C. ... Wu, J. (2017). Adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system promotes the cell apoptosis of CD133+ ovarian cancer stem cells. Oncology Reports, 37, 155-162. https://doi.org/10.3892/or.2016.5263
MLA
Long, Q., Yang, R., Lu, W., Zhu, W., Zhou, J., Zheng, C., Zhou, D., Yu, L., Wu, J."Adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system promotes the cell apoptosis of CD133+ ovarian cancer stem cells". Oncology Reports 37.1 (2017): 155-162.
Chicago
Long, Q., Yang, R., Lu, W., Zhu, W., Zhou, J., Zheng, C., Zhou, D., Yu, L., Wu, J."Adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system promotes the cell apoptosis of CD133+ ovarian cancer stem cells". Oncology Reports 37, no. 1 (2017): 155-162. https://doi.org/10.3892/or.2016.5263
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