Identification of TMPRSS2-ERG mechanisms in prostate cancer invasiveness: Involvement of MMP-9 and plexin B1

  • Authors:
    • Bide Liu
    • Xiao Gu
    • Tianbao Huang
    • Yang Luan
    • Xuefei Ding
  • View Affiliations

  • Published online on: November 28, 2016     https://doi.org/10.3892/or.2016.5277
  • Pages: 201-208
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Abstract

The relationship of TMPRSS2-ERG fusion gene with matrix metalloproteinase-9 (MMP-9) and PLXNB1 (plexin B1) in regulation of prostate cancer (PCa) aggressiveness was investigated. Fluorescence in situ hybridization (FISH) assays, qRT-PCR and western blot analysis were employed to detect the expression of TMPRSS2-ERG fusion gene, ERG, MMP-9 and PLXNB1 of 135 human tissues, which included 55 metastatic PCa cases, 50 localized PCa cases and 30 BPH cases. Then using siRNA (anti-ERG, MMP-9 and PLXNB1, respectively) downregulation of the target gene of VCaP and PC-3 cells, MTT and Transwell were performed. The results showed that the positive rate of TMPRSS2-ERG fusion was 38.1% (40/105) in total PCa samples, 47.3% (26/55) of metastatic PCa, 28.0% (14/50) of localized PCa, while 0.0% (0/30) in BPH samples. The mRNA and protein expression of ERG, MMP-9 and PLXNB1 were higher in metastatic PCa (P<0.0001), and the mRNA expression of the three genes were positively correlated with TMPRSS2-ERG fusionin PCa group (P<0.0001). siRNA transfected PCa cells can effectively downregulate the target gene expression, and we identified that MMP-9 and PLXNB1 expression were all regulated by TMPRSS2-ERG fusion gene. While only PLXNB1 contributed to TMPRSS2-ERG mediated enhancements of VCaP cell migration and invasion. The results demonstrated that PLXNB1, but not MMP-9, was the target gene directly related to TMPRSS2-ERG in PCa cell migration and invasion.
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January-2017
Volume 37 Issue 1

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Online ISSN:1791-2431

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Spandidos Publications style
Liu B, Gu X, Huang T, Luan Y and Ding X: Identification of TMPRSS2-ERG mechanisms in prostate cancer invasiveness: Involvement of MMP-9 and plexin B1. Oncol Rep 37: 201-208, 2017
APA
Liu, B., Gu, X., Huang, T., Luan, Y., & Ding, X. (2017). Identification of TMPRSS2-ERG mechanisms in prostate cancer invasiveness: Involvement of MMP-9 and plexin B1. Oncology Reports, 37, 201-208. https://doi.org/10.3892/or.2016.5277
MLA
Liu, B., Gu, X., Huang, T., Luan, Y., Ding, X."Identification of TMPRSS2-ERG mechanisms in prostate cancer invasiveness: Involvement of MMP-9 and plexin B1". Oncology Reports 37.1 (2017): 201-208.
Chicago
Liu, B., Gu, X., Huang, T., Luan, Y., Ding, X."Identification of TMPRSS2-ERG mechanisms in prostate cancer invasiveness: Involvement of MMP-9 and plexin B1". Oncology Reports 37, no. 1 (2017): 201-208. https://doi.org/10.3892/or.2016.5277