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Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway

  • Authors:
    • Xiaoyu Wang
    • Chun Liu
    • Jiaqi Wang
    • Yue Fan
    • Zhenghua Wang
    • Yuanyuan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110001, P.R. China, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China, Department of Human Resources, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China, Department of Oncology (Third Ward), The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 747-753
    |
    Published online on: December 7, 2016
       https://doi.org/10.3892/or.2016.5292
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Abstract

Transforming growth factor-β1 (TGF-β1) signaling has been shown to play a critical role in the development of epithelial-mesenchymal transition (EMT). PAI-1 is one of the most important target genes in the TGF-β/Smad signaling pathway, which can hinder the degradation of ECM composition and may promote cell invasion and migration. Oxymatrine (OM) is an alkaloid extracted from the Chinese herb Sophora flavescens Ait and has been demonstrated to inhibit the growth of various types of cancer cells including colorectal cancer. However, the anticancer effect of OM in colorectal cancer remains unclear. In the present study, we detected the expression of E-cadherin, α-SMA, FN, TGF-β1, PAI-1, Smad4, pP38 and pSmad2 in FHC, RKO and OM-treated RKO cells. We also detected pSmad2 and PAI-1 in RKO cells following the addition of SB203580 (a p38 MAPK inhibitor). The results showed that E-cadherin expression in RKO cells was significantly decreased, while PAI-1, TGF-β1, α-SMA, FN, Smad4, pSmad2 and pP38 expression levels were significantly increased in the RKO cells compared to levels in the FHC cells, which was almost completely reversed by OM. OM alleviated EMT induced in colorectal cancer via inhibition of TGF-β1/Smad signaling pathway activation by reducing P38-dependent increased expression of PAI-1. Hence, OM could be a novel therapeutic agent for colorectal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Liu C, Wang J, Fan Y, Wang Z and Wang Y: Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway. Oncol Rep 37: 747-753, 2017.
APA
Wang, X., Liu, C., Wang, J., Fan, Y., Wang, Z., & Wang, Y. (2017). Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway. Oncology Reports, 37, 747-753. https://doi.org/10.3892/or.2016.5292
MLA
Wang, X., Liu, C., Wang, J., Fan, Y., Wang, Z., Wang, Y."Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway". Oncology Reports 37.2 (2017): 747-753.
Chicago
Wang, X., Liu, C., Wang, J., Fan, Y., Wang, Z., Wang, Y."Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway". Oncology Reports 37, no. 2 (2017): 747-753. https://doi.org/10.3892/or.2016.5292
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Liu C, Wang J, Fan Y, Wang Z and Wang Y: Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway. Oncol Rep 37: 747-753, 2017.
APA
Wang, X., Liu, C., Wang, J., Fan, Y., Wang, Z., & Wang, Y. (2017). Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway. Oncology Reports, 37, 747-753. https://doi.org/10.3892/or.2016.5292
MLA
Wang, X., Liu, C., Wang, J., Fan, Y., Wang, Z., Wang, Y."Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway". Oncology Reports 37.2 (2017): 747-753.
Chicago
Wang, X., Liu, C., Wang, J., Fan, Y., Wang, Z., Wang, Y."Oxymatrine inhibits the migration of human colorectal carcinoma RKO cells via inhibition of PAI-1 and the TGF-β1/Smad signaling pathway". Oncology Reports 37, no. 2 (2017): 747-753. https://doi.org/10.3892/or.2016.5292
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